Báo cáo khóa học: C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins docx
Báo cáo khóa học: C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins docx
... al. (Eur. J. Biochem. 271) Ó FEBS 2004 13 C-, 15 N- and 31 P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins Wolfgang Eisenreich 1 , Kristina ... p.p.m.) of native and native reconstituted thioredoxin reductase (TrxR), and of the native mutant protein E159Y in the oxidized and reduced sta...
Ngày tải lên: 16/03/2014, 16:20
Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
... B-618 mutant produces about 15% of th e normal level of the proteoglycans synthesized in wild-type. The E-606 mutant produces an undersulfated form of HS proteogly- can. Finally, the C-605 mutant ... surface of the Tat-containing recombinant proteins and call other reasons to explain the differences in the mechanism of internaliza- tion between the CPP peptide and the Ta...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: A novel glycogen-targeting subunit of protein phosphatase 1 that is regulated by insulin and shows differential tissue distribution in humans and rodents pdf
... coding exon specifying a protein of 358 amino acids and a molecular mass of 38 kDa (data not shown). Comparison of the glycogen-targeting subunits of PP1 Searching mouse and rat genomic sequences in ... decreased expression of the two PP1 glycogen-targeting subunits, G L and R5⁄ PTG, in the diabetic state [33,34]. Downregulation of both the protein and mRNA levels of...
Ngày tải lên: 30/03/2014, 16:20
Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt
... Chimera 1C2 encoded residues 1–505 and 653–725 of TAP1 and residues 494–639 of TAP2 and chimera 2C1 encoded residues 1–493 and 640–703 of TAP2 and residues 506–652 of TAP1. The exchange was performed ... EcoRI site of pHbApr1neo [22] and sequenced fully in both directions. Chimera 1V2 encoded residues 1–652 of TAP1 and residues 640–703 of TAP2 and chimera 2V1 enc...
Ngày tải lên: 20/02/2014, 02:21
Tài liệu Báo cáo khoa học: Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia pptx
... switch and alters the expression of the two nuclear genome coded isoforms of CytOX V [10,32]. The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of ... Otto 2 and Narayan G. Avadhani 1 1 Department of Animal Biology and 2 Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadel...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: Comparing the substrate specificities of cytochrome c biogenesis Systems I and II docx
... permit a refinement of the conclusion of the former work. We found here low but detectable levels of the holo-forms of AXXCH variants of both cytochromes c 550 and c 552 (1 and 3% relative to ... to indicate the presence of the specific holocytochrome under investigation if the features of the spectrum, in terms of wavelength maxima, and the position and intensity of...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: C-terminal, endoplasmic reticulum-lumenal domain of prosurfactant protein C – structural features and membrane interactions ppt
... part. Urea-induced and temperature-induced unfolding CD spectra of nonreduced and reduced CTC in the presence of increasing amounts of urea are shown in Fig. 4A,B. Nonreduced CTC showed small and continuous ... using a bandwidth of 1 nm and a response time of 2 s, and 10 data points per nanometer were col- lected. Each spectrum is the average of three scans. Far-UV CD...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Microcin J25 induces the opening of the mitochondrial transition pore and cytochrome c release through superoxide generation doc
... regardless of the presence of calcium. Discussion Understanding the mechanisms of action of some types of proteins and peptides on the structural and func- tional state of mitochondria seems to ... uptake of calcium through the uniport of calcium. On the other hand, KCN and antimycin A, both inhibitors of electron transport, also inhibited the swelling induced by M...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: C-terminal truncated cannabinoid receptor 1 coexpressed with G protein trimer in Sf9 cells exists in a precoupled state and shows constitutive activity ppt
... overproduction of CB1 and the investigation of the precoupled form of this receptor with G proteins. Structural studies of the receptor ⁄ G protein complex are needed to understand the mechanism of interaction ... of 39.7 ± 1.3 pmolÆmg )1 and K d value of 2.5 nM. The truncated receptor gave a B max value of 52 ± 3.09 pmolÆmg )1 and K d value of 3.6 nM. Precoupled fo...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: A strategy for the generation of specific human antibodies by directed evolution and phage display An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom docx
... creation of large repertoires in vitro [10]. This process begins with the cloning and expression of cDNAs encoding the variable regions of the H and L chains of antibod- ies (V H and V L ), allowing ... neutralize 2 LD 50 of toxin Cn2 and 2 LD 50 of whole venom. Mutant 6009F was obtained after performing some modifications to the standard procedures of biopan- ning, s...
Ngày tải lên: 23/03/2014, 13:20