Báo cáo khóa học: Binding analyses between Human PPARc–LBD and ligands Surface plasmon resonance biosensor assay correlating with circular dichroic spectroscopy determination and molecular docking ppt
Báo cáo khóa học: Binding analyses between Human PPARc–LBD and ligands Surface plasmon resonance biosensor assay correlating with circular dichroic spectroscopy determination and molecular docking ppt
... analyses between Human PPARc–LBD and ligands Surface plasmon resonance biosensor assay correlating with circular dichroic spectroscopy determination and molecular docking Changying Yu 1,2 , Lili Chen 1 , ... and target information. Curr. Medical Chem. 10, 2327–2342. Ó FEBS 2003 Binding analyses of human PPARc–LBD to ligands (Eur. J. Biochem....
Ngày tải lên: 16/03/2014, 16:20
Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc
... is thought to bind single-stranded tails produced at DSB sites, and to form a helical nucleoprotein filament. The single-stranded DNA (ssDNA) and double-stranded Keywords DNA binding; DNA repair; Rad51; ... containing ssDNA, dsDNA, and Rad51. In the ternary complex, the homologous sequence between ssDNA and dsDNA is aligned, and the ssDNA forms a heteroduplex with a complementar...
Ngày tải lên: 19/02/2014, 06:20
Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identification of amino acid residues critical for eIF5A activity and hypusine modification doc
... proteins, and ribosome- associated proteins [28,29]. Except for those with DHS and DOHH, molecular interactions between eIF5A and other candidate binding partners have not been well characterized. Binding ... no cross-reactivity between antibod- ies to human and yeast eIF5A (compare lanes 17 and 18 in Fig. 2C, and lanes 8 and 9 in Fig. 2D). The yeast eIF5A and mo...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides pot
... Wu,H.,Lima,W.F.&Crooke,S.T.(1998)Molecularcloningand expression of cDNA for human RNase H. Antisense Nucleic Acid Drug Dev. 8, 53±61. 21. Wu, H., Lima, W.F. & Crooke, S.T. (1999) Properties of cloned and expressed human ... previously and performed in a six-well culture plate, with 1 mL of serum-free medium containing DAC-30 and ODN [29]. ODN transfection by electroporatio...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: The interaction between casein kinase Ia and 14-3-3 is phosphorylation dependent pot
... column and, after extensive washing, the pro- tein was eluted and incubated with BMH1 under kinase assay conditions, with GST used as a control (Fig. 5D). This assay shows that both 6His and GST-BMH1 ... (IVTT) product and binding assays were per- formed using GST-14-3-3 f and GST as a control. Binding was shown to increase on treatment with NaF, indicating a phospho-de...
Ngày tải lên: 23/03/2014, 04:20
Báo cáo khoa học: Binding of hemolin to bacterial lipopolysaccharide and lipoteichoic acid An immunoglobulin superfamily member from insects as a pattern-recognition receptor doc
... interacting with insect hemocytes and with bacteria. It binds to hemocytes and bacteria, a nd its binding to hemocytes inhibits hemocyte aggregation [5,13–15]. Hemolin from H. cecropia interacts with ... serum (diluted 1000-fold with binding buffer), then with alkaline phosphatase-conjugated goat anti-(rabbit IgG) Ig (Bio-Rad) (diluted 3 000-fold with binding buffer), a...
Ngày tải lên: 31/03/2014, 09:20
Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... and 8G8 for humans, SAF34, 8G8 and P4 for sheep, SAF34 and P4 for cattle, and SAF34 for mice; and for the C-ter- minal antibodies 6H4, SAF60 and SAF70 for humans, and mAbs 6H4, SAF60, SAF70 and ... the prion protein sequence (Fig. 1 and Table 1). The two bands of higher molecular masses are the di- and mono-glycosylated isoforms and the band with the lowest molecula...
Ngày tải lên: 19/02/2014, 02:20
Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc
... moiety is packed between helices 12 and 15 in the L-domain [29] and stabilized by hydrogen bonds (with Thr332 and Ser336 in helix 12) and hydrophobic interactions (with Val412 and Leu415 in helix ... ITF equilibrium binding assay [18], the fusion proteins were used in kinetic studies and ITF equilibrium binding analyses with Glc. In the adenine nucleotide (AdN) equil...
Ngày tải lên: 06/03/2014, 00:20
Báo cáo khoa học: Binding of berberine to human telomeric quadruplex – spectroscopic, calorimetric and molecular modeling studies pot
... optimization and relaxation of the solvent and potassium ions in the bulk solvent with the solute and the two inner potassium ions fixed with restraints that include 500, 100, 250, 50, 100, 25, 50 and ... center of the molecular scaffold and (c) positively charged substituents to interact with the grooves, loops and the negatively charged phosphate backbone is most likely t...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras potx
... conclu- sions that the interaction between TnC and TnI is antiparallel [10], and that the inhibitory region and the region between residues 117 and 148 are involved in the binding to N domain of TnC ... well- defined curve with an initial upward portion followed by a descending bell-shaped curve for particles with compact shape and without flexible domains, and a character...
Ngày tải lên: 16/03/2014, 18:20