Báo cáo khóa học: Binding analyses between Human PPARc–LBD and ligands Surface plasmon resonance biosensor assay correlating with circular dichroic spectroscopy determination and molecular docking ppt

... analyses between Human PPARc–LBD and ligands Surface plasmon resonance biosensor assay correlating with circular dichroic spectroscopy determination and molecular docking Changying Yu1,2, Lili Chen1, ... and targetinformation. Curr. Medical Chem. 10, 2327–2342.Ó FEBS 2003 Binding analyses of human PPARc–LBD to ligands (Eur. J. Biochem. 271) 397 Binding analyses between Human PPARc–LBD and ligands Surface ... to human PPARc ligand binding domain have been investigated for the ﬁrst time byusing surface plasmon resonance biosensor technology, CD spectroscopy and molecular docking simulation. The surface plasmon...

Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc

... isthought to bind single-stranded tails produced at DSBsites, and to form a helical nucleoprotein ﬁlament. Thesingle-stranded DNA (ssDNA) and double-strandedKeywordsDNA binding; DNA repair; Rad51; ... containing ssDNA,dsDNA, and Rad51. In the ternary complex, thehomologous sequence between ssDNA and dsDNA isaligned, and the ssDNA forms a heteroduplex with acomplementary strand of dsDNA (homologous ... loops are actually involved inDNA binding. Because aromatic residues are involvedin the ssDNA binding by bacterial single strandedDNA -binding protein (SSB) and human replicationprotein A (RPA)...

Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identiﬁcation of amino acid residues critical for eIF5A activity and hypusine modiﬁcation doc

... proteins, and ribosome-associated proteins [28,29]. Except for those with DHS and DOHH, molecular interactions between eIF5A and other candidate binding partners have not beenwell characterized. Binding ... no cross-reactivity between antibod-ies to human and yeast eIF5A (compare lanes 17 and 18 in Fig. 2C, and lanes 8 and 9 in Fig. 2D). The yeasteIF5A and most of the heIF5A mutants were readilydetectable ... IA). The human and yeast recombinanteIF5A [50], recombinant DHS [57] and DOHH [6] proteinswere puriﬁed as described previously.Yeast strains and methodsAn HHY13a strain (Table 1) with inactivation...

Báo cáo khoa học: The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides pot

... Wu,H.,Lima,W.F.&Crooke,S.T.(1998)Molecularcloningandexpression of cDNA for human RNase H. Antisense Nucleic AcidDrug Dev. 8, 53±61.21. Wu, H., Lima, W.F. & Crooke, S.T. (1999) Properties of cloned and expressed human ... previously and performed ina six-well culture plate, with 1 mL of serum-free mediumcontaining DAC-30 and ODN [29]. ODN transfection byelectroporation was carried with a Bio-Rad Gene Pulser II with ... discrepancies between RNase H activity measurements in di fferent assaysin mammals and yeast [36,43]. In mammalian cells the classI enzyme activity c ould only be measured i n a liquid assay and was...

Báo cáo khoa học: The interaction between casein kinase Ia and 14-3-3 is phosphorylation dependent pot

... column and, after extensive washing, the pro-tein was eluted and incubated with BMH1 underkinase assay conditions, with GST used as a control(Fig. 5D). This assay shows that both 6His and GST-BMH1 ... (IVTT) product and binding assays were per-formed using GST-14-3-3 f and GST as a control. Binding was shown to increase on treatment with NaF, indicating a phospho-dependent binding mecha-nism. ... lysate (seeMaterials and methods) for 90 min, and then incubated with and without NaF for anadditional 30 min at 30 °C before beingtested for interaction with 14-3-3. Lanes 1 and 2, 1% of the lysate...

Báo cáo khoa học: Binding of hemolin to bacterial lipopolysaccharide and lipoteichoic acid An immunoglobulin superfamily member from insects as a pattern-recognition receptor doc

... interacting with insect hemocytes and with bacteria. It binds to hemocytes and bacteria, a nd its binding to hemocytes inhibits hemocyte aggregation [5,13–15].Hemolin from H. cecropia interacts with ... serum (diluted 1000-fold with binding buffer),then with alkaline phosphatase-conjugated goat anti-(rabbitIgG) Ig (Bio-Rad) (diluted 3 000-fold with binding buffer), and bound alkaline phosphatase ... ). Speciﬁc binding (demonstrated by competition with unlabeled hemolin) accounted f or 70% of the h emolin binding to M. lysodeikticus, 46% of the binding to E. coli, and 25% of the binding to...

Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc

... and 8G8 for humans, SAF34, 8G8 and P4 for sheep,SAF34 and P4 for cattle, and SAF34 for mice; and for the C-ter-minal antibodies 6H4, SAF60 and SAF70 for humans, and mAbs6H4, SAF60, SAF70 and ... the prion protein sequence (Fig. 1 and Table 1). The two bands of higher molecular massesare the di- and mono-glycosylated isoforms and theband with the lowest molecular mass is nonglyco-sylated ... obtained with PrPCfrom cattle where the antibodies SAF34 and P4strongly stained the di-glycosylated band, and mAbs6H4, SAF60, SAF70 and SAF84 showed the higheststaining with the overlapping bands...

Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc

... moiety is packed between helices 12 and 15 in the L-domain [29] and stabilized by hydrogen bonds (with Thr332 and Ser336in helix 12) and hydrophobic interactions (with Val412 and Leu415 in helix ... ITF equilibrium binding assay [18], the fusion proteins were used in kineticstudies and ITF equilibrium binding analyses with Glc.In the adenine nucleotide (AdN) equilibrium binding studies, ... a-helices and stabilized by hydrogen bonds (with Thr228,Thr332, and Ser336) and hydrophobic interactions (with Val412 and Leu415). Combined with enzyme kinetic analyses, our data indicate thatthe...

Báo cáo khoa học: Binding of berberine to human telomeric quadruplex – spectroscopic, calorimetric and molecular modeling studies pot

... optimization and relaxation of the solvent and potassium ions in the bulksolvent with the solute and the two inner potassium ionsﬁxed with restraints that include 500, 100, 250, 50, 100, 25,50 and ... center of the molecular scaffold and (c) positively charged substituents to interact with thegrooves, loops and the negatively charged phosphatebackbone is most likely to interact with, and thusstabilize, ... FEBSinteraction of berberine with quadruplex. The occur-rence of sharp isobestic points at 359, 383 and 445 nmclearly indicates the existence of equilibrium in the binding. Ligand binding with DNA through...

Báo cáo khoa học: Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras potx

... conclu-sions that the interaction between TnC and TnI isantiparallel [10], and that the inhibitory region and the region between residues 117 and 148 are involvedin the binding to N domain of TnC ... well-deﬁned curve with an initial upward portion followed by adescending bell-shaped curve for particles with compactshape and without ﬂexible domains, and a characteristicplateau and a monotonic ... maintain their structural and functional characteristics [8] and calcium binding toN domain of TnC causes a conformational changethat exposes a binding site for interaction with TnI[9]. The isolated...

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