Báo cáo khóa học: Characterization of presenilin complexes f

Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx

... presenilin complex (Eur. J. Biochem. 271) 385 Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and ... or from cases with presenilin 1 missense mutations, indicated no change in presenilin 1 complex mobility. Higher molecular mass...

Ngày tải lên : 16/03/2014, 16:20

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Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-ﬁxing enzyme in the reductive tricarboxylic acid cycle ppt

... f isocitrate, and indeed, the site-speciﬁc mutagenesis of K253 to Met led to an inactive protein [23]. In addition, the alkylation of the adjacent M258 inactivated the IDH from A. vinelandii [26]. ... he residues in Ec -IDH. Expression and puriﬁcation of IDH from recombinant E. coli A high level of NADP-dependent IDH activity c ould be detected in the cell-free extra...

Ngày tải lên : 08/03/2014, 10:20

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Báo cáo khoa học: Characterization of SCP-2 from Euphorbia lagascae reveals that a single Leu/Met exchange enhances sterol transfer activity pdf

... ergosterol in the structural model of A. thaliana SCP-2. The binding mode in (A) has higher ﬁtness than the binding mode in (B). The polar amino acids at the opening between helices D and E and b-strand ... amino acids in the Triton-bound con- formation of SCP-2. The amino acids lining the cavit- ies in the E. lagascae and A. thaliana Tr-SCP-2 models and in the structu...

Ngày tải lên : 16/03/2014, 12:20

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Báo cáo khoa học: Characterization of thermostable aminoacylase from hyperthermophilic archaeon Pyrococcus horikoshii pot

... that, in PhoACY, the mutation of one zinc ligand allows the local loosening of Zn 2+ and the intervention of another Zn 2+ in the metal- binding pocket, resulting in the incorrect coordination of ... Speculated zinc-binding domain of PhoACY. Zinc ion is shown by a pink sphere. The residues related to zinc binding are shown by the balls and sticks. Characterization of t...

Ngày tải lên : 23/03/2014, 07:20

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Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate speciﬁcity by metal cations, and allosteric behavior of the peptidase pdf

... greater understanding of the characteristics of this peptidase, which would be of industrial use in the debittering of fermented foods. Results and Discussion Cloning and expression of Lc. lactis ... protein engineering of this enzyme. In the present study, the prolidase-coding gene, pepQ, was isolated from Lactococcus lactis NRRL B-1821 and cloned. Characterization...

Ngày tải lên : 30/03/2014, 04:20

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Báo cáo khoa học: Characterization of a b-N-acetylhexosaminidase and a b-N-acetylglucosaminidase/b-glucosidase from Cellulomonas ﬁmi potx

... nm using a UV trans- illuminator. Nucleotide sequences of inserts were deter- mined by primer walking and conﬁrmed by sequencing the complementary strand. The nucleotide sequences of hex20A and ... regions within the Streptomyces plicatus enzyme are found in the N-terminal module of unknown function and within the following regions of the catalytic module: a-helix 4 and th...

Ngày tải lên : 30/03/2014, 10:20

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Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... dimension. A sine function was applied in both dimensions of the time domain before Fourier transformation. Integration of 1D and 2D signals was performed using 1D winnmr and 2D winnmr software (Bruker, ... m 2 and m 3 obtained from ﬁtting with Eqn (4) ML data, as in Fig. 5, for irradiated (I) and non-irradiated (C) HeLa and MCF-7 cells. The minimum values (t m ) of...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... mainly expressed during larval metamorphosis, in the mantle edge and the digestive gland. During the reproductive cycle, expression was high in gonads during the postspawning period but not in ... cooperate with insulin to stimulate pro- liferation, polarization and mobility of imaginal disc cells in vitro. Imaginal disc growth factors may also constitute morphogenetic f...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... indicate intron 6 of 75 bp in fragments B and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and 3¢ portion of 25 bp in intron 6 in fragments F and I. The number at the right of each RT-PCR ... genera- tion of protein isoforms differing in amino acid sequence. Human DNASE1 pre-mRNA is transcribed from different transcription start sites, exons 1 an...

Ngày tải lên : 19/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... C-terminus of isoform 1 of the PM H + -ATPase of A. thaliana (AHA1) interacts with the ﬁrst 88 amino acids of PPI1 [22], indicating that the PM H + -ATPase binding site of PPI1 is localized therein. ... blue- light activation of PM H + -ATPase in guard cells of broad beans involves protein kinase-mediated phosphoryla- tion of Ser and Thr residues in the C-terminus of...

Ngày tải lên : 19/02/2014, 07:20

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