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Báo cáo khóa học: Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 ppt

Báo cáo khóa học: Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 ppt

Báo cáo khóa học: Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 ppt

... 2003 Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1 ,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 Satoshi ... the assembly of the elementary unit of fibroin in ERIt was demonstrated in this study that the elementary unit of fibroin, having the same molar ratio of H- and L -chains, and fhx/P25 as of the elementary ... important in maintaining the integrity of the elementary unit [1]. However, considering the facts that oligosaccharide chains play important roles in the assembly of vesicular stomatitis viral glycoprotein...
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Tài liệu Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells docx

Tài liệu Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells docx

... humanjoints affected by RA. Staining of sPLA2-IIA in an inactive RA tissuewas rare, and only a few synovial lining regions (dark arrowheads)showed weak immunoreactivity (A) . In an active RA ... Naiman BM,Wachtmann TS, Umland JP, Pandher K, Lapointe JM,Saha S, Roach ML et al. (2003) Impaired in ammatory and pain responses in mice lacking an inducible prosta-glandin E synthase. Proc Natl ... sPLA2-V in three active RA tissues. In all cases, intense staining of the granulation tissue in the sublining interstitium was evident. Staining of the granulation tissue and lymphaggregates...
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Báo cáo khoa học: G protein-coupled receptor-induced Akt activity in cellular proliferation and apoptosis pptx

Báo cáo khoa học: G protein-coupled receptor-induced Akt activity in cellular proliferation and apoptosis pptx

... receptor-associated death domain and of the TNF-relatedapoptosis-inducing ligand, leading to the activation of death-receptor signaling and caspase-mediated apopto-sis [59]. Activated Akt phosphorylates ... complex that includes b-arrestin, proteinphosphatase 2A and Akt. Protein phosphatase 2A inactivates Akt, thereby relieving Akt’s inhibition of glycogen synthase kinase 3b and allowing it to mediatedopamine-induced ... (also knownas tuberin) causes the dissociation of TSC2 and TSC1(also known as hamartin), relieving their inhibition of the mammalian target of rapamycin (mTOR) kinase[48]. Increased mTOR activity...
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Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf

Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf

... were of analytical grade.Yeast strains and growth media The genotypes and sources of the S. cerevisiae strains aredescribed in Table 2. The ISP deletion strain wasprepared in accordance with the ... just after the addition of ISP and Qcr10p).On the other hand, in the transition from the 500 kDaband to the 670 kDa band, a structural rearrangement of the bc1complex may occur due to the binding ... required. The available dataindicate that the accessory factor Bcs1p is involved in the binding of ISP to an immature bc1intermediate[28,29] and that Cbp3p and Cbp4p play an essential,but...
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Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc

Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc

... rearrangements include an inversion of cagQ, a duplication of cagA and cagB associated with cagA degeneration, and a more complex rearrangement comprising all cag genesexcept cagA and a second inversion of ... variations of the general gene arrange-ment. For example, some strains isolated from Ameri-can Indians have a duplication of cagA (in a nonfunctional form) and cagB inserted into the inter-genic ... Cag-T4SS, together withpotential implications for the current understanding of its mode of action. The cagPAI and the Cag type IVsecretion apparatusGene arrangement and variants of the cagPAIThe...
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Tài liệu Báo cáo khoa học: Acetylcholinesterase from the invertebrate Ciona intestinalis is capable of assembling into asymmetric forms when co-expressed with vertebrate collagenic tail peptide doc

Tài liệu Báo cáo khoa học: Acetylcholinesterase from the invertebrate Ciona intestinalis is capable of assembling into asymmetric forms when co-expressed with vertebrate collagenic tail peptide doc

... research on the interaction between WATdomains of AChE catalytic subunits and the PRADdomains of ColQ and PRiMA [38,39,45,46], the factthat G4AChE interacts with a noncatalytic subunit in nematodes ... californica AChE is 986 A ˚3.Molecular modeling of monomeric C. intestinalisAChE catalytic subunits with the PRAD domain of ColQ indicates that the WAT domain of the C. intestinalis AChE is capable ... of the acyl pocket in vertebrate and invertebrate AChEs. The line separates the vertebrate and invertebrate AChEs. The numbers at the top of the figure correspond to the amino acids in T. californica....
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt

Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt

... with the latter being dominant. The fluorescence and near-UV CD spectra point to tight packing of the aromatic residues in the core of the protein. From the near-UV CD signals and activity data as ... present in all members of the PLD superfamily. However, there is low similar-ity in the remaining parts of the molecules. Most mam-malian PLDs contain a PX and a PH domain instead of the C2 domain ... describing the chiral environment of the aromatic amino acid side chains, has a definedstructure that presents two sharp minima at 288 and 295 nm, and two maxima at 274–283 nm (wide) and 290 nm (sharp).Storage...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf

Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf

... Kaneko1, Yuanying Ding1, Kazuhiro Ogawa2,*,Miki Yoshizawa1, Masaki Kawamura1, Kazuhisa Takeda1, Tadashi Yoshida3 and Shigeki Shibahara11 Department of Molecular Biology and Applied ... HepG2 and HeLa cellswere cultured for 48 h under normoxia or hypoxia, and then harves-ted. The microsome fraction was prepared and used for the assay of HO activity. The data are means ± SEM of ... norm-oxia (Fig. 6A, C), and was shortened to 6 h under hyp-oxia. The decreased levels of HO-2 mRNA may be in part due to the enhanced degradation of the HO-2mRNA.Functional analysis of the HO-1 and...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

... N-terminal and b-he licaldomains participate in binding of the polysaccharide,pullulan.Comparison of the active sites of the model of IPU and Dex4 9A The active site structures of t he model of ... GGTGCTgAGCTCAAGTGTGACTTtcGTCTACSacIW402F CCGGTGGTcGAcTTTGGTTtcACGCCCSalIE273Q ACGTACTGCTgTCCGGAAAGtACtCCATGGCCGCScaID353N TCCAATCCGTtAGTCTGgCCaTAGAACGCGCMscIE356Q GGAGAATGGTgCCAGGGTACATTTcCAATCCGTCAKpnID372N ... catalyst of pK a 5.9, and that the adjacent Glu180 is negatively charged, raising the p K a of the general acid catalyst [23]. I t is likely t hatcatalytic residues of IPU adopt a similar catalytic...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx

Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx

... 5¢-CTGGGAGGAGACAACAGCCTGGCAATC-3¢ for His120Asn, 5¢-TGGGTTGATGCCAATGCTGACATCAAC-3¢ for His145Asn and 5¢-TGACATCGACACACCCC-3¢ for Asn149Asp.Fluorescence spectra and thermal inactivationstudiesFluorescence ... agmatinase activity of the Asn149Asp vari-ant, it is clear that the interactions of arginase II withl-arginine and agmatine are greatly altered by replace-ment of this residue with aspartate. ... [9].Certainly, if an interaction between Asn149 and the a- carboxylate group of arginine were also operative for arginase II, both the lack of arginase activity aswell as the resistance of the Asn149Asp...
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