Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new affinity label for probing the structure and function of glutathione transferases potx

... S -(2,3-Dichlorotriazinyl )glutathione A new affinity label for probing the structure and function of glutathione transferases Georgia A. Kotzia and Nikolaos E. Labrou Laboratory of Enzyme Technology, Department ... Zeta, Phi, Tau and O mega [3,4,9]. Whereas Zeta, Theta and Omega classes of GSTs are found in plants and animals, the large Phi and Tau...

Ngày tải lên: 16/03/2014, 16:20

... USA) and flowjo software (TreeStar, Ashland, OR, USA). Mice and teratoma formation C57BL ⁄ 6J mice and MCH:ICR mice were purchased from CLEA Japan (Tokyo, Japan). All of the mice were main- tained ... On the other hand, the northern blotting and quantitative real-time PCR analysis also showed that the expression of differentiation marker genes such as fgf5, gata4 and gat...

Ngày tải lên: 07/03/2014, 00:20

... com- parative sequence searches in the Mascot database (Table 1). The MSDB identification number, the theo- retical molecular mass, the theoretical pI, the sequence coverage and the MASCOT score are ... in the development and progression of CRC. Further functional and clinical analysis of the proteins is necessary to elucidate their precise role in the process of...

Ngày tải lên: 23/03/2014, 03:20

... 33, 7 1A. 4 Nakao S, Takenaka T, Maeda M, Kodama C, Tanaka A, Tahara M, Yoshida A, Kuriyama M, Hayashibe H, Sakuraba H et al. (1995) An atypical variant of Fabry’s disease in men with left ventricular ... 288–293. 5 Nakao S, Kodama C, Takenaka T, Tanaka A, Yasum- oto Y, Yoshida A, Kanzaki T, Enriquez AL, Eng CM, Tanaka H et al. (2003) Fabry disease: detection of undi- agnosed hemodia...

Ngày tải lên: 30/03/2014, 03:20

... indicates a mass of 11 kDa. Amino-acid sequence of GHP and molecular mass The complete amino-acid sequence (Fig. 2) of GHP was determined by a combination of automated Edman degradation and tandem ... 32–48, peaks appeared at differences of 16, 32, 48 and 64 mass units from the major peak at 1943.0 Da (Fig. 4A) . The peak at m ⁄ z 1959.0 Da can theoretically be inter...

Ngày tải lên: 08/03/2014, 08:20

... called TIBAQ (Text -and- Inference Based Answering of Questions, cf. (Haji~ov@ and Sgall, 1981)) with no pre-arranged data base and with the capacity of self- -enriching by information drawn ... (present, past and future), mood (indicative and imperative), and voice (active and passive). As concerns notation, usually several kinds of infor- mation are collapsed in...

Ngày tải lên: 09/03/2014, 01:20

... procedure The assay was run as a three-step assay: initial incubation of the sample and probe, addition and incubation of the sample and acceptor beads in the plate wells, and addition of donor beads ... thus confirm that the assay specifically measures HSF1 DNA-binding activity. Analytical range and precision The analytical range of the assay was evaluated usi...

Ngày tải lên: 18/02/2014, 14:20

... recent years, a number of studies have inves- tigated integrating emotions and music in certain media applications. For example, Ishizuka and Onisawa (2006) generated variations of theme mu- sic ... RMSE(V) and 0.64 in RMSE (A) . Note that RMSE0.5 means that there is roughly one quarter (25%) error in the va- lence/arousal values as they range from [-1,1]. Note that the...

Ngày tải lên: 20/02/2014, 05:20

... Xi~ and Xi, are called the primary, secondary and the j-th heads of A, respectively. The compositional semantic features of the mother node A can be represented as an ordered list of the ... Score Function In general, a particular semantic interpretation of a sentence can be characterized by a set of lexical categories (or parts of speech), a synt...

Ngày tải lên: 20/02/2014, 21:20

... TGACGTCCTTTGGATGAAAC-3¢ (reverse); C-terminus 2, 5¢-GGAGGTGACGAAGGAGAAGATAACGC-3¢ (for- ward), 5¢-TTAATCGGCCGGATCGTTCTTTTCACTACC TTTACTGTCTTC-3¢ (reverse); mid-domain, 5¢-GGACAT GACGATGCTGGTGAGG-3¢ ... (apparent) K d values for aegyptin–collagen interaction were calculated by non-linear regression analysis of the binding data with graphpad prism software (GraphPad Software, La Jolla, C...

Ngày tải lên: 22/03/2014, 21:20