Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new afﬁnity label for probing the structure and function of glutathione transferases potx

... S-(2,3-Dichlorotriazinyl )glutathione A new afﬁnity label for probing the structure and function of glutathione transferases Georgia A. Kotzia and Nikolaos E. LabrouLaboratory of Enzyme Technology, Department ... Zeta, Phi, Tau and O mega [3,4,9]. Whereas Zeta, Theta and Omega classes of GSTs are found in plants and animals, the large Phi and Tau classes a re unique to plants [9]. In maize ( Zea m ays L),42 ... at the A and B chains are 26.67 A ˚2 and 49.26 A ˚2,andtheB-factors of S atoms are 38.14 A ˚2 and 79.30 A ˚2, respect-ively. It is therefore reasonable to propose that conform-ational...

Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx

... USA) and flowjo software (TreeStar, Ashland, OR, USA).Mice and teratoma formationC57BL ⁄ 6J mice and MCH:ICR mice were purchased fromCLEA Japan (Tokyo, Japan). All of the mice were main-tained ... On the other hand, the northern blotting and quantitative real-time PCR analysis also showed that the expression of differentiation marker genes such asfgf5, gata4 and gata6 was not increased ... 1283–1297.24 Sakaki-Yumoto M, Kobayashi C, Sato A, Fujimura S,Matsumoto Y, Takasato M, Kodama T, Aburatani H,Asashima M, Yoshida N et al. (2006) The murinehomolog of SALL4, a causative gene in...

Báo cáo khoa học: Comparative proteomic analysis identiﬁes proteins associated with the development and progression of colorectal carcinoma pot

... com-parative sequence searches in the Mascot database(Table 1). The MSDB identiﬁcation number, the theo-retical molecular mass, the theoretical pI, the sequencecoverage and the MASCOT score are ... in the development and progression of CRC. Further functional and clinical analysis of the proteins is necessary to elucidate their precise role in the process of CRC and the formation of metastases.Experimental ... surpassed only by lung and prostate neoplasms in men, and lung and breast can-cers in women [1,2]. As in most malignant diseases,early diagnosis and especially detection of metastasesare of...

Báo cáo khoa học: Active-site-speciﬁc chaperone therapy for Fabry disease Yin and Yang of enzyme inhibitors pptx

... 33,7 1A. 4 Nakao S, Takenaka T, Maeda M, Kodama C, Tanaka A, Tahara M, Yoshida A, Kuriyama M, Hayashibe H,Sakuraba H et al. (1995) An atypical variant of Fabry’sdisease in men with left ventricular ... 288–293.5 Nakao S, Kodama C, Takenaka T, Tanaka A, Yasum-oto Y, Yoshida A, Kanzaki T, Enriquez AL, Eng CM,Tanaka H et al. (2003) Fabry disease: detection of undi-agnosed hemodialysis patients and ... mice.ASSC therapy for Fabry disease inhumans The clinical proof -of- concept for ASSC therapy hasbeen investigated in cardiac Fabry disease by Frustaci and colleagues [48]. Galactose, a less...

Báo cáo khoa học: GHP, a new c-type green heme protein from Halochromatium salexigens and other proteobacteria potx

... indicates a mass of 11 kDa.Amino-acid sequence of GHP and molecularmass The complete amino-acid sequence (Fig. 2) of GHPwas determined by a combination of automatedEdman degradation and tandem ... 32–48, peaks appeared at differences of 16, 32, 48 and 64 mass units from the majorpeak at 1943.0 Da (Fig. 4A) . The peak at m ⁄ z1959.0 Da can theoretically be interpreted as the oxi-dation product ... shows the MALDI MS ⁄ MS data for the precursor ion at 1975.0 Da. There is clearevidence for the presence of the further oxidationTable 1. Mass and sequence analysis of selected peptides obtained...

Báo cáo khoa học: "TOWARDS A NEW TYPE OF ANALYSE " pptx

... called TIBAQ (Text -and- Inference Based Answering of Questions, cf. (Haji~ov@ and Sgall, 1981)) with no pre-arranged data base and with the capacity of self- -enriching by information drawn ... (present, past and future), mood (indicative and imperative), and voice (active and passive). As concerns notation, usually several kinds of infor- mation are collapsed in a single abbrevi- ation, ... is ambiguous between masculine-inanimate and masculine-animate noun (N l, 4 sg § 1 sg). (In the majority of the other cases of the inflection of masculine nouns, the ambiguity in animateness...

Tài liệu Báo cáo khoa học: TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses pdf

... procedure The assay was run as a three-step assay: initial incubation of the sample and probe, addition and incubation of the sample and acceptor beads in the plate wells, and addition of donorbeads ... thusconﬁrm that the assay speciﬁcally measures HSF1DNA-binding activity.Analytical range and precision The analytical range of the assay was evaluated usingknown concentrations of recombinant human ... hD1 hAutoradiographyAdd D beads and incubateD A O2Read AlphaLISA signal at 615 nmHSEFig. 1. Comparison of EMSA and TransLISA for the detection of HSF1–DNA binding activity. (A) Schematic...

Tài liệu Báo cáo khoa học: "MemeTube: A Sentiment-based Audiovisual System for Analyzing and Displaying Microblog Messages" pdf

... recent years, a number of studies have inves-tigated integrating emotions and music in certain media applications. For example, Ishizuka and Onisawa (2006) generated variations of theme mu-sic ... RMSE(V) and 0.64 in RMSE (A) . Note that RMSE0.5 means that there is roughly one quarter (25%) error in the va-lence/arousal values as they range from [-1,1]. Note that the main reason the accuracy ... February 7th 2011. The resulting video ex-presses negative valence and neutral arousal. After checking the posts, we have learned that it is be-cause the Japanese volcano Mount Asama has con-tinued...

Tài liệu Báo cáo khoa học: "GPSM: A GENERALIZED PROBABILISTIC SEMANTIC MODEL FOR AMBIGUITY RESOLUTION" pptx

... Xi~ and Xi, are called the primary, secondary and the j-th heads of A, respectively. The compositional semantic features of the mother node A can be represented as an ordered list of the ... Score Function In general, a particular semantic interpretation of a sentence can be characterized by a set of lexical categories (or parts of speech), a syntactic struc- ture, and the semantic ... composing the compositional semantics of S, the features of VP and NP will be placed in the first and second slots of the feature structure of S, respectively. Because not all children and all features...

Báo cáo khoa học: Aegyptin displays high-afﬁnity for the von Willebrand factor binding site (RGQOGVMGF) in collagen and inhibits carotid thrombus formation in vivo ppt

... TGACGTCCTTTGGATGAAAC-3¢ (reverse); C-terminus2, 5¢-GGAGGTGACGAAGGAGAAGATAACGC-3¢ (for- ward), 5¢-TTAATCGGCCGGATCGTTCTTTTCACTACCTTTACTGTCTTC-3¢ (reverse); mid-domain, 5¢-GGACATGACGATGCTGGTGAGG-3¢ ... (apparent)Kdvalues for aegyptin–collagen interaction were calculatedby non-linear regression analysis of the binding data withgraphpad prism software (GraphPad Software, La Jolla,CA, USA). Assays were performed ... assistance with peptide synthesis, and KarineReiter for assistance with analytical SEC-MALS-HPLC. We are thankful to Drs Joan C. Marini and Wayne Cabral [National Institute of Child Health and Human...

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