Báo cáo khóa học: Phospholipase C, protein kinase C, Ca 2+ /calmodulin-dependent protein kinase II, and redox state are involved in epigallocatechin gallate-induced phospholipase D activation in human astroglioma cells ppt

... 2004 Phospholipase C, protein kinase C, Ca 2+ /calmodulin-dependent protein kinase II, and redox state are involved in epigallocatechin gallate-induced phospholipase D activation in human astroglioma cells Shi ... 2004). Abbreviations: CaM ki nase II, Ca 2+ /calmodulin-dependent protein kinase II; DCFH, 2 ¢,7¢-dichlorofluoresc...

Ngày tải lên: 16/03/2014, 16:20

... occurring mainly within domain III of ETA-A. Cell-free endosomes preloaded in vivo with ETA intraluminally processed and extraluminally released intact ETA and ETA-A in vitro in a pH-dependent and ... [22]. The antibody against KX 5 KDEL also binds to undefined endosomal proteins distinct from ETA, both in control and in toxin-injected rats, whose levels have been shown to be...

Ngày tải lên: 18/02/2014, 04:20

... unknown. Binding of cGMP during deactivation of Pabc Next, we investigated whether cGMP binds to Pabc during deactivation of Pabc to Pabcc. Binding of cGMP may be involved in Pabc deactivation in two ways: ... change is indeed involved in PDE regulation. Suppression of cGMP binding during activation of Pabcc Two possible stages for cGMP binding to Pabc are expected in PDE regu...

Ngày tải lên: 06/03/2014, 00:21

... by Fernandez-Recio and coworkers [30] as integrated in the modeling package ICM (Molsoft LLC, San Diego, CA, USA). This first protocol included a pseudo-Brownian rigid-body docking, an extended force ... constants obtained by fitting all time courses to the random-order cleavage model as described in Materials and methods, under the constraints that k 306 is minimally in uenced by he...

Ngày tải lên: 19/02/2014, 13:20

... (1559delT) were replaced with serines, the aggregation disappeared and instead this modified mutant protein formed a noncovalently associ- ated dimer, strongly indicating that these cysteine residues ... medium and the rest was polyubiquitinated, followed by degradation in the proteasome. SDS ⁄ PAGE and analysis by sucrose-density-gradient analysis indicated that TNSALP (1559delT) fo...

Ngày tải lên: 19/02/2014, 17:20

... jB-luc) in Jurkat cells by cAMP elevating agents including FSK (A), db-cAMP (B), and Br-cAMP (C). (D) Inhibition of TNFa- induced HIV-LTR-luc activation in Jurkat cells by FSK and db-cAMP. (E and F) ... PKA, cAMP-dependent protein kinase; PKAc, catalytic subunit of PKA; FSK, forskolin; db-cAMP, dibutyryl cAMP; Br-cAMP, 8-Bromo-cAMP; CREB, cAMP response element binding prot...

Ngày tải lên: 08/03/2014, 10:20

... reti- noids and steroids [1–3]. In mammals, two different groups of cellular retinoid-binding proteins with dis- tinctive binding properties have been identified, the cellular retinol-binding proteins ... (CRBPs) and the cel- lular retinoic acid-binding proteins (CRABPs). CRBPs, including CRBPI, CRBPII, CRBPIII and CRBPIV, show greatest binding affinity for retinol and retinal, but do...

Ngày tải lên: 16/03/2014, 22:20

... of dematin containing 84 amino acids of the core domain and complete headpiece domain mediated binding with the DH domain of human Ras-GRF2 (Fig. 1). In contrast, a bait construct containing ... N-ter minal PH domain, followed by the coiled coil (cc) and IQ motifs, a single DH domain that is closely linked to an other PH domain, REM and CDB motifs, and a C-terminal Cdc25 exchanger...

Ngày tải lên: 17/03/2014, 11:20

... physiological and pathological roles beyond thrombosis and hemostasis in humans [10]. However, in rodents, including rats and mice, PCI is detected only in the testes and ovaries, and not in the liver ... serine proteases are involved, and its molecular targets may include APC and thrombomodulin–thrombin [6], which play roles in the anticoagulant protein C pathway...

Ngày tải lên: 22/03/2014, 21:21

... b- D- thiogalactopyranoside; MBP, myelin basic protein; p70S6K, p70 ribosomal S6 kinase; PDK1, phosphoinositide-dependent protein kinase- 1; PKA, protein kinase A; PKB, protein kinase B; PKC, protein kinase C; ... sequence within the T-loop is also highly conserved in the Ca 2+ -and calmodulin-dependent protein kinase family (CaMK) to which TSSK3 is classified [1]...

Ngày tải lên: 23/03/2014, 11:20