Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx

... Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus Ronnie Machielsen and John van der OostLaboratory of Microbiology, ... iron- and zinc-containing alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furio-sus. J Bacteriol 181, 1163–1170.12 Shimizu Y, Sakuraba H, Kawakami R, Goda S, Kaw-arabayasi ... were obtained from Invitrogen(Paisley, UK) and New England Biolabs (Ipswich, MA,USA). Pfu Turbo and T4 DNA ligase were purchased from Invitrogen and Stratagene (Amsterdam, the Netherlands),respectively....

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... TCA GGG CAC GAC ACA CAT CCC C; and reverse primer, GAT CGG TAC CTC ATT ACA GAGGAG GGA TAT GGG GAA C. The PCR reaction wasdone as described above. The ampliﬁed PCR product wasinserted into the ... oxidase, Japanese patent 61115488.37 Yamada Y, Tawara Y & Yoshika H (1983) Production of heat-resistant polyphenol oxidase, Japanese patent60062980.38 Abdel-Raheem A & Shearer CA (2002) ... investigated fungaltyrosinases, from both a structural and a functionalpoint of view, are from Agaricus bisporus [10] and N. crassa [1]. Also, a few bacterial tyrosinases havebeen reported, of...

Báo cáo khoa học: Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding ppt

... Ct-Aspf1 (5¢-GTCGTCTTGGATCCTCTCGAGTCTCAATGAGAACACAGTCTCAAGTC-3¢). These primers contained BstEII and BamHI sites and were used to generate a fragment that wascloned in the same sequencing and ... Aspf1 wasgenerated by RT–PCR ampliﬁcation from a preparation of A. fumigatus mRNA obtained as described [22]. The pri-mers used were: Nt-Aspf1 (5¢-GTCGTCTTGCGGTCACCTGGACATGCATCAACGAACAG-3¢) and ... Production and characterization of a noncytotoxicdeletion variant of the Aspergillus fumigatus allergenAspf1 displaying reduced IgE bindingLucı´ a Garcia´-Ortega1, Javier Lacadena1,...

Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... forward)25¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ PsCIPK (degenerate reverse)35¢-CTTAT(C ⁄ G)AACAAGGAA (A ⁄ C)AATTTC-3¢ PsCBL (degenerate forward)45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ ... (degenerate reverse)55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward)65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ PsCIPK (3¢UTR reverse)75¢-CGAGCTCACTGCCTCTCAAC-3¢ PsCBL (5¢UTR forward)85¢-ACTCGTAGC-ACAGAGACAGAG-3¢ ... 1, of EF1 is replaced by amino acid A (Fig. 1B). The EF1 and EF2 are 22 amino acids apart,whereas EF2 and EF3, and EF3 and EF4 are 25 and 32 amino acids apart, respectively (Fig. 1B). The cal-cineurin...

Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-afﬁnity substrate for H+/peptide cotransporters pdf

... Gly-Gln, Ala-Ala, Ala-Ala-Ala, Lys-Lys, d-aminolevulinic acid, cefadroxil,Gly, Pro-Ala, 8-aminooctanoic acid and Gly-Sar were from Sigma-Aldrich (Deisenhofen, Germany). Tert. butyloxycar-bonyl ... h. The amount of Bip-Pro in the extracellular uptake mediumwas quantiﬁed according to the laboratory standard HPLC(La-ChromÒ; Merck-Hitachi, Darmstadt, Germany) with a diode array detector and ... byunlabeled Bip-Pro itself, but also by well known sub-strates of H+⁄ peptide cotransporters, such as Gly-Sar,Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-Ala, Ala-Ala-Ala,d-aminolevulinic acid, cefadroxil...

Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulﬁde reductase from Pyrococcus horikoshii Implications for the disulﬁde metabolism of anaerobic hyperthermophiles doc

... the enzyme at a disadvantage.Physiological role of CoADR and NAD(P)Hin Pyrococcus The maintenance of low intracellular levels of cysteinein organisms has been attributed to the avoidance of the ... form), a signiﬁcant amount of oxidase activity can beobserved in the presence of additional substrate-levelFAD (Table 2). The kcat appobtained in the presence of 100 lm NADH and FAD and 115 ... correlates well with the catalytic constantobserved for the CoADR reaction.Thermostability and thermoactivity of the CoADRphCoADR is stable for months at both )80 °C and )20 °C, and has half-lives...

Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

... determined according to [56] usingBSA as a protein standard. The DNA and amino acidsequence analyses and prediction of the molecular masseswere performed with the programs mapdraw and proteanin dnastar ... forming the iminelinkage with the coenzyme. Enzymes of the b-familycatalyse mainly b-replacement or b-elimination reac-tions. The d-alanine aminotransferase and the alanineracemase family are the ... lineages of paralogous proteins. The a- family includes enzymesthat catalyse transformations of amino acids in which the covalency changes are limited to the same carbonatom that carries the amino...

Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the speciﬁc and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf

... moleculesFig. 9. The proposed mechanism of catalysis of the b-aryl ether cleavage enzyme.Fig. 8. Mass spectra of guaiacylglycerol and guaiacol, products of the b-aryl ether cleavage reaction. The reaction ... excess of water was added and the resultantprecipitate was collected as DHP-GOU.Isolation of the fungi and enzymeActivity of the b-aryl ether cleavage enzyme was assayed asdescribed in a previous ... Seiichiro Ikeda1, Shinya Kajita1, Masaya Nakamura2 and Yoshihiro Katayama11Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei,...

Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

... using an intensifying s creen, at )80 °C.RNA for Northern analyses was isolated as describedabove and separated in a 1.3% agarose/formaldehyde gel. The RNA was b lotted to a nylon membrane (ZetaProbe,Bio-Rad) ... for details on preparation of the Trxaf®nity c olumn). Among the Chlamydomonas proteins thatwere retained on the column after loading of a proteinextract and extensive washing, was a major protein ... MareÂchal, P.,Miginiac-Maslow, M. & Meyer, Y. (1994) Arabidopsis thalianaNADPH thioredoxin reductase: cDNA characterization and expression of the r e combinant protein in Escherichia...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... in the Caryophyllaceae, Sambucaceae,Cucurbitaceae, Euphorbiaceae, Phytolaccaceae and Poaceae [1]. Although many are potentially useful inagriculture and medicine because of their antiviralproperties ... RIPnamed pulchellin. It exhibits speciﬁcity for galactose and galactose-containing structures, can agglutinatehuman and rabbit erythrocytes, and kills mice and the microcrustacean Artemia salina ... displayed hemagglutination and toxicitytoward mice (data not shown), additional efforts tocleanly isolate the isoform were not successful and further characterization was abandoned. A chromato-focusing...

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