Báo cáo khoa học: Characterization of the flavin association in hexose oxidase from Chondrus crispus pot

... cleavage of the terminal Met was not unexpected, the loss of the alanine residue was initially surprising. However, the literature revealed several examples in which the presence of proline as the ... significant role in the catabolism of the branched-chain amino acids or of methionine. Identification and mutation of residues affecting substrate specificity There...

Ngày tải lên: 06/03/2014, 00:21

... with the first 88 amino acids of PPI1 [22], indicating that the PM H + -ATPase binding site of PPI1 is localized therein. Indeed, fusion proteins containing the first 88 amino acids of PPI1, linked ... suggested by the parallel increase of the number of FC-binding sites [18–20]. As to auxin, a soluble auxin receptor has been reported to bind and activate the PM H + -ATPase...

Ngày tải lên: 19/02/2014, 07:20

... of the genes. In SNO1, the 199th G from the 5¢ terminus of the open reading frame was deleted to result in a frame-shift and appearance of stop codons in the downstream. As a result, a protein ... Glutamine was effective in protecting the enzyme from inactivation and its effect was again dose-dependent, suggesting that inhibition by DON occurs at the active sit...

Ngày tải lên: 19/02/2014, 12:20

... binding factors. The introduction of mutation in one of the Ets-binding sequences greatly decreased its promoter activity, suggesting that the transcription of the a3 integrin gene in these cells ... including Ets, GATA, and MyoD/E-box binding factors. The introduction of mutation into one of the putative Ets- binding sequences suppressed the promoter activity. In...

Ngày tải lên: 21/02/2014, 03:20

... suggested a single cut at the C9–C10 double bond of the ring-bearing side of 3-OH-c-carotene in combination with several cleavage options in the linear half of the molecule, namely at the C9¢–C10¢,C7¢–C8¢ ... temperature of 340 °C, which was maintained for an addi- tional 5 min. The He carrier gas flow was maintained at 1mLÆmin )1 using a split flow of 1 : 20. The sp...

Ngày tải lên: 07/03/2014, 03:20

... shown in Fig. 2. Several proteins are visible in regions of the second dimension that cor- respond to the activity bands in the BN gel. Five pro- tein spots from the upper band, corresponding to the high ... arrows). Owing to the horizontal smear, however, the presence of the B14 subunit in the lower band cannot be ascer- tained at this point. A cross-reaction...

Ngày tải lên: 07/03/2014, 09:20

... [7]. tRNA-dependence of pppGpp synthesis Binding states of tRNAs It was important to determine the binding states of tRNAs in ribosomal complexes that stimulated pppGpp synthesis by SF as tRNAs could either ... retained on the filter in the presence of SF (Table 2). The effect was specific to SF, as incu- bation of unacylated tRNA with recombinant ribo- somal protein L10 o...

Ngày tải lên: 07/03/2014, 16:20

... structure. The confor- mations of residues 8–9 are ill defined in the peptides. Thus, the introduction of a prolinoamino acid in the C-terminal tail of SP does not affect the overall structure of the ... space beside the absence of c-turn minima. Discussion The nonsubstituted proline analogue [Pro11]SP is only a weak competitor of SP binding sites. Reintroducing...

Ngày tải lên: 08/03/2014, 02:21

... nonheated samples, the mature form of GluV8 could be renatured even after exposure to heat in the presence of SDS. Role of N-terminal Val69 in processing of the GluV8 proform Finally, the role of N-terminal ... for the correct folding of GluV8, indicating its role as an intramolecular chap- erone. Furthermore, the four amino acid residues at the C-terminus of...

Ngày tải lên: 16/03/2014, 06:20

... cyanidin; 100 nm bilirubin and 100 nm biliverdin. Under the condi- tions of the assay, bilirubin is freely soluble in the buffer [58]. The presence of these inhibitors in the assay medium may interfere ... antibody A in the presence of increasing concentrations of bilirubin. BSP uptake was assayed to track the progress of the antibody-induced inhibition. Figure...

Ngày tải lên: 20/02/2014, 01:20