Báo cáo khoa học: Cell type-speciﬁc transgene expression of the prion protein in Xenopus intermediate pituitary cells ppt

... December2005)doi:10.1111/j.1742-4658.2006.05118.x The cellular form of prion protein (PrPC) is anchored to the plasma mem-brane of the cell and expressed in most tissues, but predominantly in the brain, including in the pituitary ... suggesting that the expression of the GFP–PrPCfusion protein in the inter-mediate pituitary is coregulated with these proteins. The POMC and p24d1 ⁄ 2 protein levels were similar in the ALs of ... animal.Biosynthesis of newly synthesized GFP–PrPCfusion protein in Xenopus intermediate pituitary cells To investigate the biosynthesis of GFP–PrPC, we mon-itored the fate of the newly synthesized...

Báo cáo khoa học: CpG and LPS can interfere negatively with prion clearance in macrophage and microglial cells pdf

... employed the compound suraminto inhibit the de novo synthesis of PrPSc[45,46].Indeed, the increase in RML-PrPSc in J774 cells wasthereby prevented, which strengthens the hypothesis of transient ... through epithelialcolon cells in the presence of differentiated M -cells may enable prions to gain ﬁrst access to the lympho-reticular system [19]. Furthermore, migrating intestinaldendritic cells, ... 2007)doi:10.1111/j.1742-4658.2007.06105.x Cells of the innate immune system play important roles in the progression of prion disease after peripheral infection. It has been found in vivo and in vitro that the expression of the cellular...

Báo cáo khóa học: Nerve growth factor mediates activation of the Smad pathway in PC12 cells doc

... transfection of PC12 cells wasperformed using LipofectAMINETM(Life TechnologiesInc.) according to the manufacturer’s protocol. 293T cells were maintained in minimum Eagle’s medium (MEM)containing ... cells. Referring to Smad4, there are signiﬁcant levels of protein in the nucleus of both cell lines already in the absence of ligand. Cycloheximide treatment for 1 h prior to the addition of NGF indicated ... component of TGF-b1 signaling in PC12 cells. Smad2 phosphorylationwasanalysedinparentalPC12cellsandinstablePC1 2cells expressing TbRII-wt under the control of a doxycycline-inducible promoter. Figure...

Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

... NaV1.2. The increase in ionic currents induced by coexpression of the human b1Bcould result from an increase in the number of channels present in the membrane, No,anincreaseintheprobability of ... are indicated by solid thin lines (b1)andshadedthinlines(b1B), and the unidenti-ﬁed 3¢ untranslated region of b1Bis indicatedusing the thin interrupted broken line. The stop codon is indicated ... expression in human tissues. (A) The presence of b1B in Purkinje cells (large arrowheads) and in their processes (small arrowheads) of the cerebellum. (B) Speciﬁc b1Blabeling was abolished in the...

Báo cáo khoa học: "Speakers’ Intention Prediction Using Statistics of Multi-level Features in a Schedule Management Domain" ppt

... focus of the utterance. Table 1. Speech acts and their meanings Speech act Description Greeting The opening greeting of a dialogue Expressive The closing greeting of a dialogue Opening ... May.…Prediction of user’s intentionIdentification of system’s intentionReducing the search space of an ASRIt is changed into 12:40. The date is changed.Is it changed into 4 May?…It is changed into ... consists of a speech act and a concept sequence (Levin, 2003). In the semantic form, the speech act represents the general intention expressed in an utterance, and the concept sequence captures the...

Báo cáo khoa học: Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3 ) from zebraﬁsh (Danio rerio) pot

... binding protein; FABP3, heart-typefatty acid-binding protein; CRBP, cellular retinol binding protein; CRABP, cellular retinoic acid binding protein; ILBP, intracellularlipid binding protein; ... oocyte; tissue-speciﬁc expression; ciselement; linkage mapping.Intracellular fatty acid-binding proteins (FABP) and the related cellular retinol and retinoic acid binding proteins(CRBP and CRABP, ... the brain, distaltubule cells of the kidney, stomach parietal cells, lactatingmammary gland, ovary, testis and placenta [reviewed in 7,29], but is absent in the liver, white fat and intestine[29,37–43]....

Tài liệu Báo cáo khoa học: Pyrimidine-speciﬁc ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc

... [48].Docking of ribose and Ca2+into the binding site of SsCU-NH was performed using, as a starting reference, the position of the sugar and of the metal in the crystallo-graphic template 1YOE. The ... accessibility of the resi-dues was calculated using the program naccess [68], byrolling a probe atom of 1.40 A˚on the van der Waals sur-face of the protein model. The analysis of cavities in the proteins ... modelled in each binding site of SsCU-NH, and then the structure of the resulting complex was optimized to allow a betteraccommodation of the ligands in their binding sites and todecrease steric hindrance....

Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx

... reinforce the argument that the basic domain should be exposed at the surface of the Tat-containing recombinant proteins and call other reasonsto explain the differences in the mechanism of internaliza-tion ... (A) Incubation of the cell with T at±GFP fusion protein without (plain line) or with heparinase treat-ment (dotted line). (B) Incubation of the cell with ¯uorescein Tat CPP without (plain line)or ... amounts of the rhodamine-labeled Tat peptide, as indicated in the ®gure.Fig. 2. FACS analysis of Tat peptide andTat±GFP fusion protein uptake in HSexpressing or de®cient cell lines. Plain linesin...

Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf

... region of the 1H,15N-HSQC spectrum of the C-terminal domain of human eRF1 illustrating the presence of two conformational states of the protein. Fig. S2. Plot of the number and distribution of the NOEs ... beendetermined in solution. The overall fold and the structure of the b-strandcore of the protein in solution are similar to those found in the crystalstructure. The structure of the minidomain (residues ... conformers of the C-terminaldomain of human eRF1.Table S1. Differences in the experimental restraints usedfor the structural determination of the two conformers of the C-terminal domain of human...

Báo cáo khoa học: Cell biology, regulation and inhibition of b-secretase (BACE-1) potx

... mutation of these cysteine residuesincreases the release of the BACE-1 ectodomain into the medium [13]. The stability and turnover of BACE-1, like that of the low-density lipoproteinreceptor, ... space of time. The inhibition of b-secretaseactivity in vivo remains one of the most viable strategies for the treatment of Alzheimer’s disease, although progress in getting inhibitors to the clinichas ... BACE-1 expression through the stress-activated protein kinase pathway [84]. The activation of cyclin-dependent kinase 5 also leads to increasedlevels of BACE-1 mRNA and protein in vivo and in vitro,...

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