Báo cáo khoa học: Cell type-specific transgene expression of the prion protein in Xenopus intermediate pituitary cells ppt

... December 2005) doi:10.1111/j.1742-4658.2006.05118.x The cellular form of prion protein (PrP C ) is anchored to the plasma mem- brane of the cell and expressed in most tissues, but predominantly in the brain, including in the pituitary ... suggesting that the expression of the GFP–PrP C fusion protein in the inter- mediate pituitary is coregulated wi...

Ngày tải lên: 16/03/2014, 14:20

... employed the compound suramin to inhibit the de novo synthesis of PrP Sc [45,46]. Indeed, the increase in RML-PrP Sc in J774 cells was thereby prevented, which strengthens the hypothesis of transient ... through epithelial colon cells in the presence of differentiated M -cells may enable prions to gain first access to the lympho- reticular system [19]. Furthermore,...

Ngày tải lên: 07/03/2014, 05:20

... transfection of PC12 cells was performed using LipofectAMINE TM (Life Technologies Inc.) according to the manufacturer’s protocol. 293T cells were maintained in minimum Eagle’s medium (MEM) containing ... cells. Referring to Smad4, there are significant levels of protein in the nucleus of both cell lines already in the absence of ligand. Cycloheximide treatment fo...

Ngày tải lên: 16/03/2014, 16:20

... Na V 1.2. The increase in ionic currents induced by coexpression of the human b 1B could result from an increase in the number of channels present in the membrane, N o ,anincreaseinthe probability of ... are indicated by solid thin lines (b 1 ) andshadedthinlines(b 1B ), and the unidenti- fied 3¢ untranslated region of b 1B is indicated using the thin interrupted brok...

Ngày tải lên: 16/03/2014, 23:20

... focus of the utterance. Table 1. Speech acts and their meanings Speech act Description Greeting The opening greeting of a dialogue Expressive The closing greeting of a dialogue Opening ... May. … Prediction of user’s intention Identification of system’s intention Reducing the search space of an ASR It is changed into 12:40. The date is changed. Is it changed in...

Ngày tải lên: 17/03/2014, 02:20

... binding protein; FABP3, heart-type fatty acid-binding protein; CRBP, cellular retinol binding protein; CRABP, cellular retinoic acid binding protein; ILBP, intracellular lipid binding protein; ... oocyte; tissue-specific expression; cis element; linkage mapping. Intracellular fatty acid-binding proteins (FABP) and the related cellular retinol and retinoic acid binding proteins (CRBP a...

Ngày tải lên: 31/03/2014, 07:20

... [48]. Docking of ribose and Ca 2+ into the binding site of SsCU-NH was performed using, as a starting reference, the position of the sugar and of the metal in the crystallo- graphic template 1YOE. The ... accessibility of the resi- dues was calculated using the program naccess [68], by rolling a probe atom of 1.40 A ˚ on the van der Waals sur- face of the...

Ngày tải lên: 18/02/2014, 17:20

... reinforce the argument that the basic domain should be exposed at the surface of the Tat-containing recombinant proteins and call other reasons to explain the differences in the mechanism of internaliza- tion ... (A) Incubation of the cell with T at±GFP fusion protein without (plain line) or with heparinase treat- ment (dotted line). (B) Incubation of the cell...

Ngày tải lên: 21/02/2014, 03:20

... region of the 1 H, 15 N-HSQC spectrum of the C-terminal domain of human eRF1 illustrating the presence of two conformational states of the protein. Fig. S2. Plot of the number and distribution of the NOEs ... been determined in solution. The overall fold and the structure of the b-strand core of the protein in solution are similar to those found...

Ngày tải lên: 06/03/2014, 11:20

... mutation of these cysteine residues increases the release of the BACE-1 ectodomain into the medium [13]. The stability and turnover of BACE-1, like that of the low-density lipoprotein receptor, ... space of time. The inhibition of b-secretase activity in vivo remains one of the most viable strategies for the treatment of Alzheimer’s disease, although progress...

Ngày tải lên: 07/03/2014, 00:20