Báo cáo khoa học: The role of glucose 6-phosphate in mediating the effects of glucokinase overexpression on hepatic glucose metabolism pdf

... following exposure to calcium iono- phore, indicating that they were correctly targeted into secretory granules even in the absence of serglycin. In the absence of serglycin, the fates of the serglycin-dependent ... dependent on serglycin. Together, these findings indicate that the reduced amounts of neutral proteases in the absence of serglycin is not caused by m...

Ngày tải lên: 07/03/2014, 11:20

... investigated the relationship between defects in flavin cofactor homeostasis and the function of mitochondrial FAD-binding enzymes. Correlation of these has been demonstrated in human pathologies, including ... strain. Construction of SDH1 promoter–lacZ fusion in the flx1D mutant strain To isolate an flx1D mutant strain in which the regulatory region placed upstream of t...

Ngày tải lên: 23/03/2014, 07:20

... for input data. The urea-induced unfolding curves were obtained by plotting the ellipticity at 222 nm against urea concentration. To estimate the urea concentration of midpoint of the unfolding ... only the N-terminal 29 and C-terminal 43 residues (amino acid positions at 31–59 and 148–190) of EspA correspond- ing to the binding interface of CesA could be clearly solved....

Ngày tải lên: 30/03/2014, 16:20

... 336–346 ª 2005 The Authors Journal compilation ª 2005 FEBS The role of glucose 6-phosphate in mediating the effects of glucokinase overexpression on hepatic glucose metabolism Linda Ha ¨ rndahl 1 , ... effect on either glucokinase binding or on the metabolism of [2- 3 H ]glucose, which approximates the rate of glucose phosphorylation (Fig. 3...

Ngày tải lên: 16/03/2014, 14:20

... post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were fixed on day 4 of differentiation and stained with hematoxylin and eosin. One of two independent ... biologically relevant role of miR-27 in the regulation of adipose tis- sue functions in vivo, we examined the expression of miR-27 in the genetically obese ob ⁄ ob mi...

Ngày tải lên: 18/02/2014, 08:20

... thus blocking protein synthesis and leading cells to death. The current model proposed for the mechanism of antitumour action of BS-RNase is based on the ability of the protein to resist the neutralizing ... any effects of 1–10 m M concentrations of bacitracin (Sigma), a known inhibitor of PDI [21], on the dimerization reaction. Likewise, an anti-PDI serum (Stres...

Ngày tải lên: 20/02/2014, 11:20

... obtained by other groups who also point to the role of the connecting loop of the b-hairpin of defensins. A combination of mutational analysis [35] and structural analysis of the plant defensin Rs-AFP1 ... observations, it is not possible to infer whether the key role of the b-hairpin loop of MGD1 (loop 3) in antimicrobial activity of MGD1 is of general val...

Ngày tải lên: 20/02/2014, 11:20

... pH-activity curve of API The pH-activity profiles depending on NaCl concentration were determined as part of the investigation into the shielding from solvent of the electrostatic interaction between ... position. In the struc- tural deviation, the solvent ASA of the side-chain of His210 increased with the decrease in size of the side-chain at residue 169 (T...

Ngày tải lên: 21/02/2014, 03:20

... found a set of DIDO1 homologues in the context of DNA binding and chromatin regulation. These homologues include members of the CpG binding protein (CGBP) family of proteins that bind DNA at unmethylated ... attributed to these structural ele- ments, very little is known about their role in DIDO activity. The combination of domains (domain shuffling) is a driving force i...

Ngày tải lên: 07/03/2014, 21:20

... strongly on the levels of expression of Adxp but not Adrp and therefore that the concentration of Adxp is the major factor controlling pregnenolone synthesis in recombinant yeast. In conclusion, ... in ergosta-5-eneol content than in the pregnenolone accumulating strain CA14/pCD63. This result is consistent with observation of the absence of inhibition by pregnen...

Ngày tải lên: 08/03/2014, 08:20