Báo cáo khoa học: The role of glucose 6-phosphate in mediating the effects of glucokinase overexpression on hepatic glucose metabolism pdf

Báo cáo khoa học: A role for serglycin proteoglycan in granular retention and processing of mast cell secretory granule components ppt

... following exposure to calcium iono-phore, indicating that they were correctly targeted into secretory granuleseven in the absence of serglycin. In the absence of serglycin, the fates of the serglycin-dependent ... dependent on serglycin. Together,these ﬁndings indicate that the reduced amounts of neutral proteases in the absence of serglycin is not caused by missorting into the constitutive path-way of secretion, ... were incubatedfor 120 min in the presence of 2 lm of the calcium iono- Role of serglycin in secretory granule assembly F. Henningsson et al.4910 FEBS Journal 273 (2006) 4901–4912 ª 2006 The...

Báo cáo khoa học: Succinate dehydrogenase ﬂavoprotein subunit expression in Saccharomyces cerevisiae – involvement of the mitochondrial FAD transporter, Flx1p ppt

... investigated the relationship between defects in ﬂavin cofactor homeostasis and the function of mitochondrial FAD-binding enzymes. Correlation of these has been demonstrated in human pathologies,including ... strain.Construction of SDH1 promoter–lacZ fusion in the ﬂx1D mutant strainTo isolate an ﬂx1D mutant strain in which the regulatoryregion placed upstream of the SDH1 coding region wasfused in- frame ... respirationwas not reduced. By increasing the phenylsuccinateconcentration, and therefore reducing the succinatetransporter activity, we obtained a signiﬁcant reduction in succinate respiration. Conversely,...

Báo cáo khoa học: A pH-dependent conformational change in EspA, a component of the Escherichia coli O157:H7 type III secretion system potx

... for input data. The urea-induced unfolding curves were obtained byplotting the ellipticity at 222 nm against urea concentration.To estimate the urea concentration of midpoint of the unfolding ... only the N-terminal 29 and C-terminal 43 residues (amino acidpositions at 31–59 and 148–190) of EspA correspond-ing to the binding interface of CesA could be clearlysolved. The other regions ... proteins into host cells[16].Although there is information available concerning the roles and structural properties of EspA ﬁlaments,to date, the conformation and the thermodynamicproperties of...

Báo cáo khoa học: The role of glucose 6-phosphate in mediating the effects of glucokinase overexpression on hepatic glucose metabolism pdf

... 336–346 ª 2005 The Authors Journal compilation ª 2005 FEBS The role of glucose 6-phosphate in mediating the effects of glucokinase overexpression on hepatic glucose metabolism Linda Ha¨rndahl1, ... effect on either glucokinase binding or on the metabolism of [2-3H ]glucose, which approximates the rate of glucose phosphorylation (Fig. 3E,F). The distribution of glu-cokinase between the nucleus ... 5B). The saturation of phosphorylase inactivation by Glc6P could explain the lack of further inactivation by S4048 at 35 mm glucose (Fig. 4).Comparison of the metabolic effects of glucokinase overexpression...

Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt

... post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were ﬁxed on day 4 of differentiation and stained with hematoxylin and eosin. One of two independent ... biologicallyrelevant role of miR-27 in the regulation of adipose tis-sue functions in vivo, we examined the expression of miR-27 in the genetically obese ob ⁄ ob mice. The expression levels of both miR-27a ... blocking the transcriptional induction of PPARc and C ⁄ EBPa orby preventing preadipocytes from entering the stage of adipogenesis determination or commitment. The tran-scriptional repression of...

Tài liệu Báo cáo khoa học: A role for the intersubunit disulﬁdes of seminal RNase in the mechanism of its antitumor action docx

... thusblocking protein synthesis and leading cells to death. The current model proposed for the mechanism of antitumouraction of BS-RNase is based on the ability of the proteinto resist the neutralizing ... any effects of 1–10 mMconcentrations of bacitracin(Sigma), a known inhibitor of PDI [21], on the dimerizationreaction. Likewise, an anti-PDI serum (Stressgen) had noinhibitory effects on the ... MSSGmonomers conserved the acquired dimeric structure uponcell internalization. This was considered a necessary condi-tion to attribute to the dimerization event a role in the antitumour action...

Tài liệu Báo cáo khoa học: Key role of the loop connecting the two beta strands of mussel defensin in its antimicrobial activity docx

... obtained by other groupswho also point to the role of the connecting loop of the b-hairpin of defensins. A combination of mutationalanalysis [35] and structural analysis of the plant defensinRs-AFP1 ... observations, it is not possible to infer whether the key role of the b-hairpin loop of MGD1 (loop 3) in antimicrobial activity of MGD1 is of general value in the mechanism of action of defensins. ... permeation[20,21], the mode of action of defensins requires an initialbinding step on the outer membrane. The way this contacttakes place and the molecular features of the proteininvolved...

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... pH-activity curve of API The pH-activity proﬁles depending on NaCl concentrationwere determined as part of the investigation into the shielding from solvent of the electrostatic interactionbetween ... position. In the struc-tural deviation, the solvent ASA of the side-chain of His210increased with the decrease in size of the side-chain atresidue 169 (Table 1 and Fig. 3A). However, the ASAs of Asp113 ... size of the side-chain at residue 169 and the pK2showed a clear linear relationship (Fig. 3B). The pK2increased as the size of the residue at position 169 increased. The effect of the size of...

Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx

... found a set of DIDO1 homologues in the context of DNA bindingand chromatin regulation. These homologues includemembers of the CpG binding protein (CGBP) family of proteins that bind DNA at unmethylated ... attributed to these structural ele-ments, very little is known about their role in DIDOactivity. The combination of domains (domain shufﬂing)is a driving force in Eukarya in the acquisition of KeywordsCGBP; ... to maintain transcription. The PHF3 protein was recovered in initial analysesand also contains a TFS2M domain showing the samearchitecture as the DIDO1 long isoform, although lack-ing the dPHD....

Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx

... strongly on the levels of expression of Adxp but not Adrp and thereforethat the concentration of Adxp is the major factorcontrolling pregnenolone synthesis in recombinant yeast. In conclusion, ... in ergosta-5-eneolcontent than in the pregnenolone accumulating strainCA14/pCD63. This result is consistent with observation of the absence of inhibition by pregnenolone in the pregneno-lone ... resulting in anincrease in intracellular sterol trafﬁcking. It is unclear, then,whether the corresponding increase in pregnenolone pro-duction results from an increase in free sterol concentrationor...

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