... Toyobo (Osaka, Japan), and New EnglandBiolabs (Beverly, MA, USA). All other reagents were of analytical grade from Nacalai Tesque (Kyoto, Japan) andWako Pure Chemical Industries (Osaka, Japan).Culture ... Similar results were obtainedwith various concentrations of NADPH and fixed con-centrations of pyruvate. These indicate a sequentialmechanism for the NMAADH reactions. In fact, the data obtained ... use ammonia as a substrate and was distinctfrom alanine dehydrogenase [18] and N-methylalaninedehydrogenase found by Lin & Wagner [17]. Lysineand ornithine were inert as an amine substrate;...