Báo cáo khoa học: Recombinant hemoglobinbG83C-F41Y An octameric protein pdf

... pH 7.0 in H 2 O and at 29 °C: exchangeable proton resonances in the CO form (A) and hyperfine- shifted and exchangeable proton resonances in the deoxy form (B). Tetramer–octamer transition of Hb ... Tetramer–octamer transition of Hb bG83C-F41Y FEBS Journal 273 (2006) 230–241 ª 2005 The Authors Journal compilation ª 2005 FEBS 241 Recombinant hemoglobin bG83C-F41Y An octameric protein Co...

Ngày tải lên: 16/03/2014, 14:20

... precursor can be used as a template for structural and functional studies of INSL3 because it can be prepared through recombinant expression more conveniently and, as well, many analogues can also ... human INSL3, the refolded precursor was first A BC Fig. 1. (A) Amino acid sequence and nucleotide sequence of the recombinant human INSL3 precursor. The B-chain and A-chain are shown in red a...

Ngày tải lên: 30/03/2014, 01:20

... with mouse anti-human Golgin 97 (A) serum, mouse anti-human LAMP2 serum (D), mouse anti-human CD63 serum (G), mouse anti-human MPR serum (J) or mouse anti-human EEA1 serum (M). Primary antibodies ... approximately 65 kDa (e.g. Fig. 5A, lane 2), three protein bands of approximately 45 kDa, and one protein band of approximately 36 kDa. As all these proteins were visu- alized with antiserum...

Ngày tải lên: 18/02/2014, 08:20

... each substrate: mutan (a-1,3- and a-1,6-glu- can), nigeran (a-1,3- and a-1,4-glucan), soluble starch (a-1,4- and a-1,6-glucan), pustulan (b-1,6-glucan), laminarin (b-1,3-glucan), carboxymethilcelullose ... a-1,3-glu- cans with a-1,6 branches. The observed optimum pH and thermostability are in the range obtained for the T. harzianum CECT2413 mutanase [25] and other enzymes from T. harzianum CC...

Ngày tải lên: 19/02/2014, 16:20

... and rZP4 mutants in Sf9 cells infected with recombinant baculoviruses Native ZPGs are synthesized as transmembrane proteins, processed at a site N-terminal to their trans- membrane regions, and ... precipitated with anti- FLAG gels and detected by antibody to FLAG (Fig. 6A, lane 6 in the right panel) but not by antibody to His (Fig. 6A, lane 6 in the left panel). The bands indicated by ......

Ngày tải lên: 07/03/2014, 05:20

... Software, San Diego, CA). Acknowledgements This work was supported by grants from the Heart and Stroke Foundation of Canada (grant T4134) and the Canadian Institutes of Health Research (grant MT11270). ... cell extracts but 80% inhibition with recombinant protein, and this difference may arise from different characteristics of native HepG2 protein and recombinant protein, such as the...

Ngày tải lên: 07/03/2014, 09:20

... sentences and to provide for top-down computation. Also, we have done an analysis of 80 sentences with multiple parses from our data base to evaluate the per- formance of this metric, and have ... as an auxiliary rather than as a main verb when both parses are possible can be realized by using a K of 0, a reward, when picking up an auxiliary verb. Similarly, a K of 2, a penalty,...

Ngày tải lên: 08/03/2014, 18:20

... and ikebana ('flower arranging'). The word ichibana (El) does not exist in Japanese. His explanation 'number one' was correctly translated (not shown here) as ichiban. ... translation under normal circum- stances. For example, Engfish I see should be translated as naruhodo rather than watashi ga miru, Japanese wakarimashita should be I under- stand rather than .....

Ngày tải lên: 08/03/2014, 18:20

... the anchor node as 3sg which has the effect (due to the re- entrancy in the unanchored tree) of instantiating the agr feature at the subject node as 3sg. Anchored elementary trees are translated ... between the an- chor of the tree and the nodes it imposes con- straints on. If all dependencies relevant to the parser can be captured in this way then, once an elementary tree has been...

Ngày tải lên: 08/03/2014, 21:20

... Purification and analysis of recombinant shewasin A. Wild-type shewasin A was produced in E. coli in soluble form, fused to an N-terminal His-tag. (A) HisTrapHP chromatogram. Recombinant shewasin ... purification of recombinant shewasin A and the active site mutant in E. coli Wild-type shewasin A and shewasin A_(D37A) were trans- formed into E. coli BL21(DE3). The method of recombi- nan...

Ngày tải lên: 14/03/2014, 22:20