Báo cáo khoa học: Recognition of DNA modified by trans-[PtCl2NH3(4hydroxymethylpyridine)] by tumor suppressor protein p53 and character of DNA adducts of this cytotoxic complex potx

... some aspects of DNA modification by trans-PtHMP and how this modifica- tion affects recognition by tumor suppressor protein p53 of the consensus nucleotide sequence to which this protein specifically ... in DNA by the adducts of BBR3464 and cisplatin pro- duce differential responses to p53 protein activation and recognition. It is therefore of great...

Ngày tải lên: 16/03/2014, 14:20

... solution contain- ing 30 lm of Tb 3+ and 1.5 lm of protein was set as the starting point. The stock solution of 10–100 mm CaCl 2 with the same concentration of Tb 3+ and protein was gradually added ... affini- ties and metal-binding properties of CaM [14] and other EF-hand proteins, such as the nonstructural pro- tease domain of rubella virus [15]. We have shown that an...

Ngày tải lên: 07/03/2014, 00:20

... The protein profile of the serum samples from the 98 patients with CRC and the 80 healthy controls were extracted by magnetic beads and examined by MALDI-TOF-MS. The data were analyzed by Bio- marker ... proteome profiles of CRC were generated by using the combination of MALDI-TOF MS and WCX magnetic beads as well as pattern recognition soft- ware in our study. T...

Ngày tải lên: 25/10/2012, 11:18

... The presence of two forms of spermosin in H. roretzi sperm. (A) SDS/PAGE of the puri®ed spermosin. SDS/PAGE gave a band of 28 kDa under red ucing conditions and two b ands of 33 and 40 kDa under ... showed two bands of 33 and 4 0 kDa under nonreducing c onditions (Fig. 3A). The N-terminal sequence determination of these bands (Fig. 3B) revealed that the 28-kDa protein is...

Ngày tải lên: 17/03/2014, 11:20

... both immature and mature dendritic cells, the amount of the 34 kDa form of CD63 disappeared gradually during the chase period, concomitantly with the appearance of isoforms of CD63 of  50 and  70 ... maturation of dendritic cells, the number of MHC class II-peptide complexes that are generated is increased, both by a transient up-regulation of synthesis as well as by...

Ngày tải lên: 08/03/2014, 02:20

... (Hovy and Maier, 1993) for a survey). However, the definitions are rather vague and they are often recognized to be underspecified (Moore and Pollack, 1992; Fukumoto and Tsujii, 1994). This ... manner of event are also contained in this category, since a manner accompanies an event. The notion of the manner is continuous to the means since the means and manner of an...

Ngày tải lên: 08/03/2014, 05:21

... derived by G only in two possible ways and only by using the first string components of a set of nonterminals N(0 of fan-out two. In this way the derivation of the substring w(X)w(2) w(p) by ... Outline of the proof. Let (U, C) be an arbitrary in- stance ofthe 3SAT problem, where U = {Ul, , up} is a set of variables and C = {Cl, c,} is a set of clauses; each...

Ngày tải lên: 31/03/2014, 06:20

... inhibition of elastase-2 by a 1 -PI and interference by sulfated polysaccharides. Fig. S2. Progress curves for the inhibition of elastase-2 by eglin c and interference by sulfated polysaccharides. This ... inhibition and reactivation by the con- comitant action of two modifiers I and X. Plots of the reaction rate as a function of the concentration (m M) of two m...

Ngày tải lên: 16/02/2014, 14:20

... Absence of these proteins causes a random insertion of the P1 protein and a cytadher- ence-negative phenotype [13]. P1 is encoded by MPN141 and both, P40 and P90 by MPN142. These genes are organized, ... insight into the function of this protein com- plex, the subunits had to be characterized in detail. The N-terminal end of P90 was determined by Edman degradation....

Ngày tải lên: 19/02/2014, 18:20

... eutropha was shown to be very similar to that of standard hydrogenases, with a Fe atom liganded by 1 CO and 2 CN – [16], and the binding of an hydride to Ni and Fe after H 2 reduction has recently ... inactivation by air. The hydrogenase activity of the purified proteins, assayed by H 2 uptake in the presence of benzyl violo- gen, was about fivefold lower in the mutant prot...

Ngày tải lên: 20/02/2014, 01:20