Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf

... degradation of the protein. The latter half of the kinase inhibitory region and the entire extended SH2 subdomain form a single a-helix. The mapping of the true SH2 domain, and the location of its ... within SOCS3. The PEST sequence is an insertion in the SH2 domain Analysis of the secondary structure of SOCS3, and sequence alignments...

Ngày tải lên: 16/03/2014, 14:20

... mea- sured in solution. Furthermore, it resembles the mean of an ensemble of 20 structures and thus indicates the dynamics of the protein, leading to changes in second- ary structure according to the ... [19]. Therefore, the error of the secondary structure change is much lower than the error of the absolute secondary structure determination. The...

Ngày tải lên: 07/03/2014, 04:20

... and and in the 20±25 loop, as well as in the P2¢ and P 3¢ positions of the binding loop. The residues with the greatest Dd NH values are Thr16 and, more interestingly, Gln35. The majority of the ... equential, and long-range (in cluding medium-range) NOE di stance restraints per residue for SGCI (A) and SGTI (B); angular order parameters [26] of / and w tor...

Ngày tải lên: 08/03/2014, 10:20

... production of VP2 was prepared by insertion of amplified sequences into the HindIII and BglII sites, and the plasmid for production of VP3 was pre- pared by insertion of amplified sequences into BglII and KpnI ... test interac- tions of the minor structural proteins in cells, contribu- tions of individual hydrophobic domains to their membrane affinity and to r...

Ngày tải lên: 16/02/2014, 09:20

... (1997) Anticoagulant synergism of heparin and activated protein C in vitro. Role of a novel anticoagulant mechanism of heparin, enhancement of inactivation of factor V by activated protein C. J. Clin. ... constants of FVa inactivation as UFH, but pentasaccharide did not inhibit FVa inactivation. Analysis of these data in the context of the 3D structures of APC a...

Ngày tải lên: 19/02/2014, 13:20

... significance of the recombinant S. lividans cells expressing the R73V ⁄ R84A variant from the viewpoint of industrial and medical applications. Results Expression of the R73V ⁄ R84A variant of CYP105A1 ... Wat560, 587 and 593 in the binding site were included in the calculation. Cluster analysis was performed on the docked results of 100 runs using a root-mean-s...

Ngày tải lên: 15/03/2014, 23:20

... different plant and animal sources, with more than 400 isoforms being obtained from amphibian species. This article discusses the antibacterial activity and mode of action of the N-terminal region of ... different initial surface pressures (p 0 ), the peptide (0.1–2 lm) was injected into the subphase, and the increment in surface pressure of the LPS film upon interc...

Ngày tải lên: 16/03/2014, 00:20

... isomers of cyclosarin [30] and those of p-nitrophenol analogs of sarin and soman [25]. Therefore, it was of interest to examine the stereoselectivity of cyclosarin and soman hydro- lysis exerted by ... constants (k cat ⁄ K m )of1 0 5 )10 6 m )1 Æmin )1 [16]. The catalytic effi- ciency of PON1 in the hydrolysis of sarin and soman and the possibility to re-in...

Ngày tải lên: 16/03/2014, 13:20

... hydrolysis, and improving the Fig. 1. (A) The crystal structure of TaLcc1 as a surface model. Domain A is presented in blue, domain B in green, and domain C in yellow. The N-glycans are shown ... Asn555. The glycan on Asn202 was in the groove between the b-bar- rels of domains A and B (Fig. 2B), and had six hydro- gen bonds, three to the main chain ca...

Ngày tải lên: 14/02/2014, 18:20

... cargo binding sites, referred to as the major and minor binding sites [1]. These sites are located in the concave face of the protein near regions of invariant Trp and Asn arrays. The major binding site ... to the flexible importin-b binding domain. The importin-b binding domain is known to have an autoinhibitory function, whereby an internal NLS-like sequence comp...

Ngày tải lên: 14/02/2014, 19:20