Báo cáo khoa học: Cartducin stimulates mesenchymal chondroprogenitor cell proliferation through both extracellular signal-regulated kinase and phosphatidylinositol 3-kinase⁄Akt pathways pot

... Cartducin stimulates mesenchymal chondroprogenitor cell proliferation through both extracellular signal-regulated kinase and phosphatidylinositol 3 -kinase Akt pathways Hironori ... cartducin- stimu-lated proliferation of mesenchymal chondroprogenitor cells.Our results indicated that cartducin initiatesMAPK and PI3K signaling cascades in mesenchymal chondroprogenitor cells, ... factor, and mitogenic response to cartducin by mesenchymal chondroprogenitor cellsrequires the activation of both the ERK1 ⁄ 2 and PI3K ⁄ Akt pathways. H. Akiyama et al. Cartducin activates MAPK and...

Báo cáo khóa học: Characterization of Mesorhizobium huakuii lipid A containing both D-galacturonic acid and phosphate residues ppt

... protons and protons from a, b and c positions of 3-hydroxy fatty acids are marked with;-CH ¼ CH-, -CH3 ,and- CH2-, a, b and c, respectively. CHCl3,DMSOandH2O represent signals from solvents and ... Darvill, A.G., McNeil, M., Stevenson, T.T. &Albersheim, P. (1985) Isolation and characterization of plant cell walls and cell wall components. Methods Enzymol. 118, 3–40.30. Lowry, O.H., Roberts, ... A. Choma and P. Sowinski (Eur. J. Biochem. 271) Ó FEBS 2004Characterization ofMesorhizobium huakuiilipid A containing both D-galacturonic acid and phosphate residuesAdam Choma1 and Pawel...

Báo cáo khoa học: Glutamine stimulates the gene expression and processing of sterol regulatory element binding proteins, thereby increasing the expression of their target genes docx

... protein kinase signaling pathways, which in turn activatesSREBP processing. It has been reported that glutamine stimulates Akt phosphorylation in HepG2 cells [21] and both phosphatidylinositol 3 -kinase ... cells and Caco-2 cells were plated in 12-well plates ata density of 1.0 · 105cellsÆwell)1, cultured with DMEM for20 h, and then transfected with 200 ng of one of the reporterplasmids and ... PI3K-Akt [15,23,24] and mTORC-p70S6K1 pathways [25].Therefore, we next treated cells with the PI3K inhibi-tor LY294002 and the mTORC1 inhibitor rapamycinto assess whether these kinase pathways are...

Báo cáo khóa học: Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump Robert Saunders and Georgios Scheiner-Bobis pptx

... medium (ECGM; Promocell, Heidelberg, Ger-many) and transferred to a gelatin-coated, 94-mm cell culture dish. After 4 h, the medium and any nonadherentcells were aspirated and the medium replaced. ... smooth muscle cells of theartery. As this substance is a potent vasoconstrictor and growth promoter of vascular smooth muscle cells via theETAreceptor, and because vesicular fusion and release ... induce MAP kinase activation independently of ouabain.Ouabain was added to the cells at various concentra-tions in ECGM-SF, and the cells were further incubatedat 37 °C for 30 min. The cells were...

Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"

... medicaleducation and professionalismChristopher P Landrigan, Steven W Lockley and Charles A CzeislerDivision of Sleep Medicine, Boston, Massachusetts, USACorresponding author: Christopher P Landrigan, clandrigan@rics.bwh.harvard.eduPublished ... to be tested, and shouldbe a major focus of future work.Authors’ responseEric B Milbrandt, Babak Sarani and Louis H AlarconWe would like to thank Dr Landrigan, Dr Lockley, and DrCzeisler ... wide (i.e. thosethat involved both interns and other personnel), where awithin-subjects design was not appropriate. Data from 20interns were analyzed in Lockley and colleagues’ study, asthe...

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... cells and decreased in a-syn-expressing cells compared to control-transfectedcells. (E) Quantiﬁcation of the surface DAT bands in (B) normalizedagainst b-actin as analyzed by densitometry and ... preferentially expressed in oligodendrocytes, and ispresent in both the cell body and myelin sheets [12,31], and its putative functions related to microtubules and vesicle transport warrant further ... central core and were similar to those required for vesicle binding. Co-expression of p25a and the dopamine transporter in HEK-293-MSR cells sensitized them tothe toxicity of extracellular dopamine....

Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf

... c,e, and g). To determine their subunit compositions,bands i–vi were excised and subjected to SDS⁄ PAGEon 6% and 14% gels (Fig. 5B,C), followed by densito-metric analyses. Both band i and band ... of ADP and Mg2+ and followed by nondenaturing PAGE, the bands of theenzyme and the reactivase were markedly reducedin density, and two bands of the enzymeÆreactivasecomplexes (upper and lower) ... of DD and its subun-its (aD, bD and cD) and the reactivase (DD-R) and its subunits (aR and bR) are indicated on the right of the gels. In (C), to improve visi-bility of the bands of...

Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer ppt

... functional domains: an extracellular ligand-binding domain; a transmembranedomain; an intracellular tyrosine kinase domain; and aC-terminal regulatory domain [2]. The extracellular domain is subdivided ... mul-tiple signalling pathways, including mitogen-activatedprotein kinase, phosphatidylinositol 3 -kinase ⁄ AKT and the signal transducer and activator of transcription(STAT)3 and STAT5 pathways (Fig. ... HER3 and ERBB4 ⁄ HER4. ERBB2, ERBB3 and ERBB4show extracellular homologies, relative to the EGFR,of 44, 36 and 48%, respectively, while those for thetyrosine kinase domain are 82, 59 and 79%,...

Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

... morphogenesis, in particular proliferation, differentiation, cell cycle regulation, and apoptosis [11,45,46]. Through suppression of genesinducing terminal differentiation, apoptosis, and AP-2 proteins ... chondrocyte hypertrophy[57]. Mice lacking both Runx2 and Runx3 do not havehypertrophic chondrocytes or type X collagen-express-ing cells, showing that both Runx2 and Runx3 areimportant regulators ... facial ectoderm, and for signals from this epithelium that induce skeletogen-esis in NCCs. Zebraﬁsh embryos lacking both tfap2a and tfap2b have defects in epidermal cell survival and deﬁcient NCC-derived...

Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc

... conjunctivaepithelial Chang cells [38] and J774A.1 macrophages[39] through the type III secretion system. Also,ETA-induced human mast cell apoptosis by activationof the caspase-8 and caspase-3 pathways and downre-gulation ... reconstitution of the cytotoxic pathways within vitro cytosol and cell- free mitochondria, our datasuggest a direct interaction between ETA and cyto-solic EF-2 on the one hand, and the mitochondrialmembrane ... (the rat liver in vivomodel versus cellular in vitro systems) and ⁄ or may berelated to differences between hepatocytes and other cell types.In vivo [36] and in vitro [37] studies have shown...

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