Báo cáo khoa học: The b domain is required for Vps4p oligomerization into a functionally active ATPase potx

... AGGAACAA-3¢; forward (F13S), 5¢-AGCCCCGCCAT CACCCAGGAACAA-3¢; forward (F13Y), 5¢- TATCCCG CCATCACCCAGGAACAA-3¢; and reverse (F13), 3¢-CG TAGGTGTGGGTTGTATCGG-5¢. PCR products with blunt ... sine-bell apodization in the < /b> 15 N dimension. RNA transcripts and EMSA The < /b> probe was generated as described before [9]. Labeled RNA probe was incubated with various amounts of recom- binant pr...

Ngày tải lên: 07/03/2014, 05:20

... abnormalities a Unexpected abnormalities a Abnormalities expected by the < /b> ICU physician Abnormalities found by the < /b> radiologist Unexpected abnormalities found by the < /b> radiologist Abnormalities ... predefined major abnormalities in 2,457 daily routine chest radiographs Abnormalities Expected abnormalities a Unexpected abnormalities a Abnormalities expected by the...

Ngày tải lên: 25/10/2012, 10:39

... 5¢-GGTATTGAGGGTCGCCATGGTTATGTTC AATCACCA-3¢; reverse primer, 5¢-AGAGGAGAGTTAG AGCCTTACAAGAAGGGTCCAAAGA-3¢). The < /b> PCR product was purified, treated with T4 exonuclease to create vector-compatible overhangs and annealed ... that LlCBP3 3A has a preference for A B C Fig. 5. Substrate preferences for LlCBP3 3A at pH 6.0. (A, B) Bind- ing of LlCBP3 3A visualized by SDS-PAGE. (A)...

Ngày tải lên: 18/02/2014, 08:20

... Ohtani N, Yanagawa H, Tomita M & Itaya M (2004) Identification of the < /b> first archaeal type 1 RNase H gene from Halobacterium sp. NRC-1: archaeal RNase HI can cleave an RNA-DNA junction. Biochem ... coelicolor A3 (2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain Naoto Ohtani 1 , Natsumi Saito 1 , Masaru Tomita 1 , Mitsuhiro Itaya 1,...

Ngày tải lên: 19/02/2014, 18:20

... 5¢-TTCCTGGACACGCTGGT-3¢; and antisense, 5¢-CAATCGGGGATGTCTGAG-3¢. The < /b> b- actin primer pairs were as follows: sense, 5¢-TCGTGCGT GACATTAAGGAG-3¢; and antisense, 5¢-ATGCCAGGGT ACATGGTGGT-3¢. The < /b> 25 lL reaction ... of the < /b> primers used were as follows: P1 sense, 5¢-AGTTTCGCTCTTGTCT CCCAG-3¢; P1 antisense, 5¢-ATGGCGAAACCCTGTCTC TAC-3¢; P2 sense, 5¢-AGACAGCCGTTTTACACGCAG-3¢; P...

Ngày tải lên: 07/03/2014, 02:20

... been unambiguously established for mammalian cells. By intercalating between membrane lipids the < /b> two structurally unrelated membrane fluidizers that we selected benzyl alcohol (BA) and heptanol ... (i.e. a reduction of the < /b> steady-state DPH anisotropy value by  0.015 units) can be attained by the < /b> administration of 30 mm BA or 4.5 mm HE. The < /b> critical concentrati...

Ngày tải lên: 07/03/2014, 12:20

... data described above revealed that Lad1 acts largely, albeit with different affinities, on the < /b> same substrates as mammalian SDH, therefore suggesting that Lad1 may be a fungal orthologue of this ... contribution of Xdh1 is minor compared to that of Lad1, which is therefore of major importance for the < /b> L -arabinose catabolic pathway of H. jecorina. Because of the < /b> poo...

Ngày tải lên: 07/03/2014, 15:20

... is El; the < /b> affixed string generated by GI* that represents its structural description is El*. (El) abbaba (El*) SaABbABbAaSABbAaS Let us say that an affix covers elements in an affixed ... generates the < /b> artificial language L1 = a (b* a) *b* a. (G1) a. S • S A b. A • B A c. A ~ a d. B * b e. S ) a A noncenter-embedding affix grammar that...

Ngày tải lên: 08/03/2014, 18:20

... domain. Marking synsets with field labels has a clear ad- vantage: in general, given a polysemous word in WordNet and a particular field label, in most of the < /b> cases the < /b> word is disambiguated. ... it contains all the < /b> words of the < /b> language that do not belong to the < /b> FL. In general the < /b> quantity of application specific information is sma...

Ngày tải lên: 08/03/2014, 21:20