... 5¢-TTCCTGGACACGCTGGT-3¢;and antisense, 5¢-CAATCGGGGATGTCTGAG-3¢. The< /b> b- actin primer pairs were as follows: sense, 5¢-TCGTGCGTGACATTAAGGAG-3¢; and antisense, 5¢-ATGCCAGGGTACATGGTGGT-3¢. The < /b> 25 lL reaction ... of the < /b> primersused were as follows: P1 sense, 5¢-AGTTTCGCTCTTGTCTCCCAG-3¢; P1 antisense, 5¢-ATGGCGAAACCCTGTCTCTAC-3¢; P2 sense, 5¢-AGACAGCCGTTTTACACGCAG-3¢;P2 antisense, 5¢-CACCGAGAAATCGAAATCACC-3¢;P3sense, ... isothiocyanate-conjugatedgoat anti-(rabbit serum) as secondary antibody, incubatedwith rat anti-3MeK9H3 serum and stained with TRITC-conjugated goat anti-(rat serum) as secondary antibody, andfinally stained...