Báo cáo khoa học: Kinetic deuterium isotope effects for 7-alkoxycoumarin O-dealkylation reactions catalyzed by human cytochromes P450 and in liver microsomes Rate-limiting C-H bond breaking in cytochrome P450 1A2 substrate oxidation pdf

... Kinetic deuterium isotope effects for 7-alkoxycoumarin O-dealkylation reactions catalyzed by human cytochromes P450 and in liver microsomes Rate-limiting C-H bond breaking in cytochrome P450 ... similar for 7-OMe and 7-OEt coumarin, with P450 1A2 showing high isotope effects (8–16) and P450 2E1 yielding isotope effects of 2–4...

Ngày tải lên: 16/03/2014, 13:20

... proteins [13] and binding interactions [14]. FRAP is a conventional technique used to study the kinetic properties of proteins in a cell by measuring the fluorescence recovery rate in a bleached ... dynamic properties of proteins in the PGC determined by FCS and FRAP. A fusion protein consisting of Olvas and green fluorescent pro- tein (GFP) (Olvas–GFP) expressed in the PGC...

Ngày tải lên: 18/02/2014, 16:20

... (A). Inset: a representative experiment showing the effect of the addi- tion of cATR (in the concentrations indicated in the inset figure, in n M)onDW m , as indicated in the inset of (B). Data in ... activity in rat liver mito- chondria. J Biol Chem 265, 8444–8450. 61 Austin J & Aprille JR (1984) Carboxyatractyloside- insensitive in ux and efflux of adenine nucleotides in...

Ngày tải lên: 16/03/2014, 00:20

... carboxyles- terases and albumin, was controlled by nondenaturing PAGE and activity staining [26,68]. Kinetics of substrate hydrolysis in the absence of ligand Kinetic assays with neutral and positively and negatively charged ... actual binding site of serotonin cannot be inferred from these results. Although serotonin can bind to the active site binding locus (W82), binding to...

Ngày tải lên: 18/02/2014, 17:20

... rate of substrate cleavage. Values for k obs were obtained from single-turnover reactions at 44 °C using 166 n M ribozyme, 1.3 nM 5¢-end labeled substrate in buffer containing 135 m M KCl and 10 ... solution of 300 nm ribozyme in 10 lL H10Mg buffer containing 3.4% glycerol was preincubated for 5 min at 60 °C and P. P. Dotson II et al. Kinetics of the trans excision-splicing...

Ngày tải lên: 18/02/2014, 18:20

... Kinetic basis for linking the first two enzymes of chlorophyll biosynthesis Mark Shepherd, Samantha McLean and C. Neil Hunter Robert Hill Institute for Photosynthesis and Krebs Institute for ... implying a possible role for this protein in substrate delivery. The next step in chlorophyll biosynthesis, catalysed by magnesium protoporphyrin IX methyltransferase (ChlM in S...

Ngày tải lên: 20/02/2014, 02:21

... to A. Mattevi and M. Nardini for carrying out crystallization trials, to G. Tedeschi and A. Negri for performing MALDI-TOF analyses and N-terminal sequencing, and to G. Deho ` for helpful discussions. PVK, ... in the 20 lL aliquot (in nmol). Steady-state kinetic analyses and inhibition studies The apparent k cat and K M values of Mt-GluRS for tRNA Glu , ATP and l-Gl...

Ngày tải lên: 07/03/2014, 03:20

... 7.0) containing 1mm dithiothreitol and 20 lm PLP, and 3–60 lgÆmL )1 of the recombinant enzymes, at 37 °C for 10 min. The ranges of substrate concentrations for the measurement of kinetic parameters ... containing 20 lm PLP and 0.1% Triton X-100. The insoluble materials were eliminated by centrifugation at 15 000 g for 10 min, and subsequently 12.4 and 1.24 mgÆmL )1 of th...

Ngày tải lên: 07/03/2014, 05:20

... of enzyme kinetics, the conditions with constant activity were established. This was accomplished by preincubating hemocyanin with 5 mm SDS for 5 min before adding the substrate. Using this condition, which ... assayed in 0.1 M phosphate buffer (pH 7.0). Before initiation of the reaction by the addition of the substrate dopamine, hemocyanin was preincubated for 5 min with 5 m M S...

Ngày tải lên: 07/03/2014, 05:20

... recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacterium sp. strain HE5 Bernhard Sielaff and Jan R. Andreesen Institut ... Fe 3 S 4 ferredoxin (Fd mor ) and cytochrome P450 (P450 mor ). In this study, we purified Fd mor and P450 mor as recombinant proteins as well as FdR mor , which has been isola...

Ngày tải lên: 07/03/2014, 16:20