Báo cáo khoa học: Intermonomer cross-linking of F-actin alters the dynamics of its interaction with H-meromyosin in the weak-binding state ppt

... role in the stabilization of the proteinÆDNA complex. The arm of MC1 can also be compared with the DNA-binding b-hairpin arms of HUs, which showed high mobility relative to the core (Fig. 2). The ... structure of methanogen chromosomal protein 1 (MC1), deter- mined with heteronuclear NMR methods, agrees with its function in terms of the shape and nature of...

Ngày tải lên: 22/03/2014, 17:20

... in insulin-secreting b-cells. In order to gain more insight into the physiological role of mir-9 in insulin secretion, we have specifically addressed the link between GSIS and the regulation of ... altered in insulin-secreting b-islets during GSIS in vivo. Using b-TC-6 cells and conditions that mimic the declining phase of insulin secretion, we clearly demonstrate that...

Ngày tải lên: 06/03/2014, 00:21

... conserved glycine (Fig. 3E), it will be important to examine the impact of further enhan- cing its flexibility on the functional properties. In fact, the flexibility of the hinge region has often been ... Ribbon diagram of the minimized average structure of fowlicidin-3. (D) Superimposition of the average structures of fowlicidin-3 with fowlicidin-1. The struc- t...

Ngày tải lên: 07/03/2014, 11:20

... demonstrated that the inhibition of PAI-1 on insulin signaling is inde- pendent of uPAR-VN binding. These results constitute the first demonstration of the interaction of PAI-1 with the insulin response. Keywords: ... suggesting that the VN enhancement of insulin signaling is dependent on avb3 integrin. To assess the potential role of PAI-1 in the VN- mediated i...

Ngày tải lên: 08/03/2014, 08:20

... in the nucleus. In any case, in the hope of determining domain(s) important for the intracellular localization, we first attempted to investigate the intracel- lular distribution of a number of ... in bold. The basic residues in domain 3B are indicated by underlines. The similarity between SIK1 and SIK2 in domain 3A, the N-terminal half of domain 3, is higher...

Ngày tải lên: 30/03/2014, 15:20

... 1896–1905 ª 2006 The Authors Journal compilation ª 2006 FEBS 1905 Intermonomer cross-linking of F-actin alters the dynamics of its interaction with H-meromyosin in the weak-binding state Gyo ¨ rgy ... produce an increase in rotational motion in the environment of the Cys374 site of actin [8]. In a recent publication the authors argue that in...

Ngày tải lên: 16/03/2014, 13:20

... twice in PBSCM supplied with 100 mm glycine, and incubated for 20 min to quench further cross-linking. The quenching buffer was removed by washing twice in PBSCM, and the cells were then lysed in ... Values are the mean of six replicates. The specific uptake was determined by subtracting the uptake counts in the absence of inhibitor from the uptake counts in the...

Ngày tải lên: 14/02/2014, 21:20

... N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, both characteristic of angiopoietins. ANGPTLs do not bind to either the angiopoietin receptor Tie2 or the related protein Tie1, ... conditions leptin serves to counteract weight gain, in ammation induces a state of ‘leptin resistance’ in obese animals and humans resulting in development of hyperleptinemia....

Ngày tải lên: 14/02/2014, 22:20

... 2A). The zinc ions are required for the structural integrity of the protein, as mutation of any of the cysteine residues involved in zinc coordina- tion results in protein unfolding [22]. The ... WDR5. Consistent with a central role for the MLL1 Win motif in the interaction by WDR5, substitution of arginine 3765 with alanine in MLL1 abolishes the interac...

Ngày tải lên: 16/02/2014, 14:20

... examined using the insulin precipitation assay. In the noncatalyzed assay, the disulfides of insulin are reduced by dithiothreitol, causing the aggregation and precipitation of the B-chain of insulin, ... B-chain significantly, decreasing the lag-phase (Fig. 2A). When bacitracin was included with PDI, the lag-phase of the insulin precipita- tion increased (Fig. 2A),...

Ngày tải lên: 16/02/2014, 14:20