Báo cáo khoa học: Combined use of selective inhibitors and fluorogenic substrates to study the specificity of somatic wild-type angiotensin-converting enzyme docx

... Journal compilation ª 2006 FEBS 1781 Combined use of selective inhibitors and fluorogenic substrates to study the specificity of somatic wild-type angiotensin-converting enzyme Nicolas D. Jullien 1 , ... 1 and 2) validates the use of selective inhibitors as convenient tools to study the spe- cificity of the somatic form of ACE. Thus,...

Ngày tải lên: 16/03/2014, 13:20

... [14]. The neurotoxicity of Ab seems to depend on its ability to aggregate, and the active portion of the Ab molecule appears to be the amino acids 25–35 fragment [15,16]. Most caspase-3 inhibitors ... flow cytometry revealed the presence of a typ- ical sub-G1 peak indicative of an apoptosis ratio of 4.73%. As the duration of treatment increased to 48 and 7...

Ngày tải lên: 07/03/2014, 11:20

... is the probability of meeting the token t i in French; and n is the number of tokens in a text. 2. Deciding what is the best linguistic unit to consider. The equations introduced above use 21 tokens, ... attention to the fact that the MLR model multiplies the number of pa- rameters by J − 1 compared to the PO model. Because of this, they recommend using th...

Ngày tải lên: 08/03/2014, 21:20

... identify individuals with mild disease, thereby providing the missing data. The phenotype of XLA and the potential of BTK and ITK inhibitors The outcome of defective BTK signaling in humans has ... for BTK [32] and ITK [33,34]. ITK inhibitors may potentially be used for the treatment of inflammatory diseases [34] and, as discussed below, may also become part of...

Ngày tải lên: 14/02/2014, 18:20

... measures the total dye amount associated with the cells, including the dye adsorbed to the sur- face of the cells. To avoid complication of the uptake studies by active pumping of the dyes into the ... appear to be due to Pgp activity. Because the over-expression of MRP 1–5 did not significantly reduce the amount of TMRM taken up into the cells, the ob...

Ngày tải lên: 18/02/2014, 13:20

... protein to prevent degradation. A common drawback of such approaches is the cost of the affinity resins used to isolate the fusion protein and the proteases required to liberate the protein of interest ... the peptide is bound in a concentrated manner at the top of the column, or, if bound to the resin prior to packing the column, the salt gradient con...

Ngày tải lên: 18/02/2014, 13:20

... in SOL. These findings further support the presence of distinct pathways regulating fiber specific isoforms and metabolic enzymes, on the one hand, and mitochondrial biogenesis, on the other. Renewed ... of COX I, COX Vb and F1-ATPase b, between SOL and TA on the one hand and between PV– ⁄ – and WT TA on the other, revealed striking similarities. There were almost no di...

Ngày tải lên: 19/02/2014, 07:20

... 4-linked to the GlcNAc of a terminal Le a determinant. As the C 3a ion is at m/z 690, this excludes the possibility of the other Fuc being at the Gal next to the subterminal GlcNAc. The position of the ... downfield from the Fuc IX H5 proton. For TFiLNO, the same temperature would have resulted in total overlap of the H 2 O signal with the Fuc X and XI H5...

Ngày tải lên: 19/02/2014, 12:20

... analysed the thermal unfolding profile of lysozyme in the presence of additives. Figure 3A shows the thermal unfolding curve of lysozyme in the presence of ArgEE as m onitored by DSC. In the absence of ... to Arg is related to the ethylation of the main chain of the carboxyl group. Figure 5B shows the amount of aggregation of lysozyme at pH 6.5 and 10....

Ngày tải lên: 19/02/2014, 16:20

... Attempts to clarify the stoichiometry by adding a small amount of the purified dioxygenase to the reaction mixture with a large excess of the purified enzyme reported here to avoid the formation of 2,5-pyridinedicarboxylic ... time. The peak at 268 nm was assigned to 2,5-pyridinedicarboxylic acid based on the wavelength [10]. Purification and properties of the...

Ngày tải lên: 19/02/2014, 16:20