Báo cáo khoa học: Dual role of Nbs1 in the ataxia telangiectasia mutateddependent DNA damage response ppt

... role in the DNA damage response. In addition, the extreme COOH-terminal region (amino acids 734–754) of Nbs1 mediates the interaction of Nbs1 with ATM and the recruitment of ATM to sites of DNA ... formation of MRN foci [19–21]. The Mre11-binding domain of human Nbs1 has been localized to amino acids 682–693 in the COOH- terminal region of the...

Ngày tải lên: 16/03/2014, 13:20

... evaluations The quality of fitting of the kinetic model to the various sets of experimental data was determined by the coefficient of determination B being the ratio of the explained variation of the data ... Kinetic modelling For the derivation of the rate equations it was assumed that for the concentrations of the reactants used in the experi- ments,...

Ngày tải lên: 16/03/2014, 19:20

... effect of tyrosine phosphorylations on the binding affinity of ephrinB2(301–333) to the SH2 domain of Grb4 and to the PDZ domain of PDZ-RGS3. In the combination experiments, the two C terminal tyrosine ... then assess whether the binding of the PDZ domain of PDZ- RGS3 affects binding of the Grb4 SH2 domain to phosphorylated peptides, through studies of ternary i...

Ngày tải lên: 19/02/2014, 12:20

... GLUT8 NICD to the tailless lumenal domain of the IL-2 receptor alpha chain (TAC) protein (interleukin-2 receptor a chain) targeted the protein to intracellular membranes, indicating that its N-terminal ... lysosomal marker protein LAMP1, a finding that is in agreement with our in vitro binding data. Targeting of GLUT8 in the absence of AP1 and AP2 In order to investigate...

Ngày tải lên: 16/03/2014, 02:20

... obese mice In order to gain insights into the potential biologically relevant role of miR-27 in the regulation of adipose tis- sue functions in vivo, we examined the expression of miR-27 in the genetically ... together, these results illustrate a critical and specific role of miR-27 in the regulation of adipogenic differentiation. miR-27 prevents the inducti...

Ngày tải lên: 18/02/2014, 08:20

... operates in the presence of thrombin (e.g. the PKA inhibitor H89 reinforces the thrombin-induced Ca 2+ response when P2Y 12 is active). In addition, the present study demonstrates an important role ... significantly extend the earlier work investigating the interaction of P2Y 12 with P2Y 1 signaling [25,40]. We find that P2Y 12 enhances the thrombin-induced Ca 2+ resp...

Ngày tải lên: 18/02/2014, 16:20

... obtained by other groups who also point to the role of the connecting loop of the b-hairpin of defensins. A combination of mutational analysis [35] and structural analysis of the plant defensin Rs-AFP1 ... molecule comprising Ôresidues in the protruding domain consisting of the type VI b-turn and the first part of b-strand 3Õ (i.e. the b-hairpin loop and part...

Ngày tải lên: 20/02/2014, 11:20

... this protease at pH9 [10]. Further interest in the aromatic stacking is in the role of the electrostatic properties in enzymatic catalysis of API, and in distinguishing the functionally catalytic quadruple ... position. In the struc- tural deviation, the solvent ASA of the side-chain of His210 increased with the decrease in size of the side-chain at r...

Ngày tải lên: 21/02/2014, 03:20

... in the target recombinant protein [22]. The distribution of side chains in the sequence, such as occurs in protein folding, plays a pivotal role in determining the conformational properties of ... oxidative stress, the unfolded protein response, the heat shock and the stringent response and the activation of the DNA repair SOS system [2]. Traditionally,...

Ngày tải lên: 06/03/2014, 00:20

... identified prothrombin as one of the plasma cofactors. In incubations of U937 cells with prothrombin and Xa, plactin increased the formation of thrombin, which cleaved single-chain u-PA to afford the inactive ... activate prothrombin, plactin D increased the level of a-throm- bin (Fig. 5B). Thus, the plactin D effects were increas- ing or decreasing the formation of a-t...

Ngày tải lên: 07/03/2014, 00:20