Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx

... presence of increasing concen- trations of methyl green, a majo r groove b inding drug [41] and distamycin A, a minor groove binding drug [42]. Increasing concentrations of distamycin A resulted in ... to incubate overnight. The strip was washed with three changes of 1 · binding buffer over a period of 30 min and autoradiographed. In vitro DNase I footprinting analysis DNase I...

Ngày tải lên: 16/03/2014, 18:20

... Name Length (-mer) 5¢-TGTTTGTTTGTTTGTTTGTTTGTTTGT-3¢ GT 27 5¢-TGGTGTGTGTGGGGTGGTTGGTG-3¢ GT- G1 23 5¢-TGGGGTGTGTGGGGTGGTTGGTG-3¢ GT- G2 23 5¢-TGGGGTGTGTGGGGGGGTTGGTG-3¢ GT- G3 23 5¢-TGGTTGGGGTGGGGGGGGGGGTG-3¢ GT- G4 ... 2006 FEBS 1361 Interaction of G-rich GT oligonucleotides with nuclear- associated eEF1A is correlated with their antiproliferative effect in...

Ngày tải lên: 16/03/2014, 13:20

... the effect of HSP25 on the polymerization of intact actin and aggregation of heated actin. Interaction of HSP25 with intact actin In the first series of experiments we analyzed the effect of HSP25 ... polymerization of intact actin. (A) Effect of HSP25 and its 3D mutant on the kinetics of actin polymerization. Samples containing 1–4 l M of actin (10% of pyrene-la...

Ngày tải lên: 20/02/2014, 23:20

... sensor of nitrogen availability in B. subtilis [1,9]. The feedback-inhibited form of GS binds tightly to TnrA, preventing its binding to DNA, with the most effective feedback inhibitors of GS being glutamine ... above). The C-terminus of TnrA is required for interaction with both GlnK and GS, as well as for intracellular proteolysis Previously, it had been reported that the...

Ngày tải lên: 06/03/2014, 00:21

... protein (sequence element containing amino acids 98–110 of the flavivirus E protein) may serve as an internal fusion peptide, as it is directly involved in interactions with target membranes during ... the interactions of an N-terminal and an internal peptide sequence of the E2 structural protein of HGV ⁄ GBV-C with model mem- branes, in order to understand the possible mode...

Ngày tải lên: 07/03/2014, 17:20

... type of disease. Protein aggregation can result from external insults or aging, however, inherited forms of neurodegenera- tive diseases, such as familial Parkinson’s disease, Huntington’s disease ... control in determining the effect of native proteins on cell viability. Buffers at pH 3.3, 4.8 and 7.3 without the protein had no effect on cell viability (data not shown). Stefin...

Ngày tải lên: 07/03/2014, 17:20

... ND49 with CP is because of deletion of the interacting domain. Discussion MPs are a diverse group of non-structural proteins of plant viruses that are involved in the spread of infection from cell ... domain within the N-terminal 49 amino acids of the SeMV MP. This is in contrast to observations on CPMV MP, in which the C-terminal domain was shown to be involved in...

Ngày tải lên: 15/03/2014, 00:20

... the change in access- ible surface area upon binding being small. These thermodynamics indicate that the binding is dominated by hydrophobic interactions, which is in agreement with inclusion ... these interactions, it could be deduced that the cavity size of c-CD is suitable for GA binding, but those of a-CD and b-CD are too small. In order to determine the binding site of...

Ngày tải lên: 16/03/2014, 14:20

... I ref is the intensity in a reference spectrum recorded without presaturation. NMR spectroscopic studies of protein–protein interaction For 2D NMR spectroscopic studies of protein–protein interaction, ... [28]. This paper describes the interaction of a 15 N-labelled PSBD (residues 119–171) of the B. stearothermophilus E2p polypeptide chain with the dimer of a protein doma...

Ngày tải lên: 16/03/2014, 18:20

... this result proved that the binding of C1r to C1q is inhibited by fucoidan, consistent with the interaction of the polysaccharide with the hinge region of the collagen-like region of C1q. Discussion C1q ... well as of C1 and of the isolated collagen-like region to individual DNA strands, indicating that the collagen-like region is the main binding site of DNA. From bin...

Ngày tải lên: 16/03/2014, 23:20