Báo cáo khoa học: Kinetic study of the HIV)1 DNA 3¢-end processing Single-turnover property of integrase docx

... binding of IN to the processed DNA product. The stability of IN during the time-course of the reaction and the influence of protein aggregation are discussed. Results General features of the 3¢-end processing ... 3¢-end processing. In the second step, DNA strand transfer, a pair of processed DNA ends of the same viral DNA is inserted into the host...

Ngày tải lên: 16/03/2014, 13:20

... the logarithm of the molecular mass of the proteins in the y-axis and the elu- tion volumes (V e ) in the x-axis. Comparison of the elution volumes of the P. abyssi and E. coli ATCases with the standard ... consequence of the cooperativity toward aspartate and of the ordered binding of the substrates, first CP then aspartate [13]. These results raised the...

Ngày tải lên: 16/03/2014, 19:20

... K d is the equilibrium dissociation constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound to the ribozyme, and [ribozyme] u is the concentration of unbound ribozyme in the reaction. Measurement ... and fitting to the equation: k obs ¼ k 1 ½Eþk À1 : Measurement of the dissociation constant, K d P of the ribozyme–product complex The equilibrium di...

Ngày tải lên: 18/02/2014, 18:20

... Mg-chelatase of tobacco: identification of a Chl D cDNA sequence encoding a third subunit, analysis of the interaction of the three subunits with the yeast two- hybrid system, and reconstitution of the ... timepoint. The data for the decay of inter- mediate in the presence of ChlH were fitted to a three-parameter exponential (k ¼ 31.7 ± 9.5 s )1 ). The evolution o...

Ngày tải lên: 20/02/2014, 02:21

... denaturation of the enzyme. With the exception of the temperature- activity profile, the characteristics of the enzyme were determined from initial velocity measurements in the direction of the NADH-dependent ... in M. thermoautotrophicum [7]. Although several properties were investigated, there has been no kinetic study of the mechanism of this enzyme. Aeropyru...

Ngày tải lên: 22/02/2014, 04:20

... the protease. In the first experiment, we compared the 15 N-HSQC spectra of WNV NS2B–NS3pro(K96A) in the presence of 3 and in the presence of the Bz-nKRR-H inhibitor used in one of the crystal structure ... assignments have been reported for the WNV NS2B–NS3 protease. The poor quality of the NMR spectrum of WNV NS2B–NS3pro in the absence of inhibitors is rem...

Ngày tải lên: 07/03/2014, 02:20

... PCR’, using the first PCR product as template. To make use of the BamHI site in the vector and the XbaI site in the EhMGL1 gene, the product of the nes- ted PCR was replaced with the corresponding ... molecular ratio of 1 : 1000, and further tested for the Met-degrading activity on the basis of the detection of a-keto acid after the addi- tion of 2 mm Met (F...

Ngày tải lên: 07/03/2014, 05:20

... is the number of features, is a weight for feature , and estimates the difference between the two instances’ values at the th feature. The classes of the nearest neighbors then determine the ... consists of the val- ues for all features (the columns) and the function- chunk code for the focus word. The features de- scribe the focus word and its local context. Fo...

Ngày tải lên: 08/03/2014, 07:20

... those of the substrates used in the kinetic experiments. The production of p-nitrophenol was linear with time for the incubation period, and < 5% of the available substrate was hydrolyzed. The ... these residues stabilize the distortion of the NAG3 residue. In these interac- tions, we particularly focused on the Trp664 residue, which is located at the bottom...

Ngày tải lên: 15/03/2014, 11:20

... this study we used IR spectroscopy to study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the ... structure of 282 residues of a total of 344 have been solved [19]. The catalytic domain of PKCh possesses a high degree of homology ( 80%) with the catalyt...

Ngày tải lên: 16/03/2014, 13:20