Báo cáo khoa học: The potent inhibitory activity of histone H1 2 C-terminal fragments on furin doc

... of the porcine histone H1. 2 As there is no intron in the genes of histones, the genomic DNA from porcine liver was used as a template to clone the gene of histone H1. 2. The human and murine histone ... Journal 27 3 (20 06) 4459–4469 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 4469 The potent inhibitory activity of histone H1. 2 C-term...

Ngày tải lên: 16/03/2014, 13:20

... absence of ATP; (2) with 1 .25 lM of OsDmc1 in presence of 2. 0 m M of AMP-PNP; (3) with 1 .25 lM of OsDmc1 in presence of 2. 0 mM ATP-c-S; (4) with 1 .25 lM of OsDmc1 in presence of 2. 0 m M of ATP. C. ... at 522 nm as a function of reaction time (Fig. 4A). This was in stark contrast to the renaturation reaction, where the emission intensity at the same waveleng...

Ngày tải lên: 19/02/2014, 07:20

... neutral pH, an MG conforma- tion resembling that of the MG conformation adopted by intact a-LA at pH 2. 0 [31, 32] . The conformational properties of the OA complexes of these fragments were analyzed ...  20 -fold the CAC value of OA. Estimated values of the CAC of OA were 0.94 ± 0 .24 lm in the presence of a-LA, and 0.86 ± 0 .29 , 1 .22 ± 0 .24 and 0.93 ± 0.56...

Ngày tải lên: 06/03/2014, 09:22

... Brilliant Blue R250. 0 20 40 60 80 100 120 34567891011 12 pH of incubation Relative activity (%) PhyH PhyH-DII 0 20 40 60 80 100 120 0 123 45 Concentration of Ca 2+ (m M ) Relative activity (%) PhyH ... 0.55 500 24 . 82 27. 72 0.0540 PhyH at 20 °C 2. 81 ± 0 .28 1143 15.93 17.76 0.0155 PhyH-DII at 35 °C 1. 82 ± 0 .23 14 32 5.56 4.17 0.0 029 PhyH-DI-168PhyA at 55 °C 1...

Ngày tải lên: 14/02/2014, 15:20

... 22 10 22 19. Glutathionylation of chloroplastic GAPDH M. Zaffagnini et al. 22 6 FEBS Journal 27 4 (20 07) 21 2 22 6 ª 20 06 The Authors Journal compilation ª 20 06 FEBS The thioredoxin-independent isoform ... Zaffagnini et al. Glutathionylation of chloroplastic GAPDH FEBS Journal 27 4 (20 07) 21 2 22 6 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 21 5 activity u...

Ngày tải lên: 19/02/2014, 05:20

... 20 04, accepted 6 February 20 04) Eur. J. Biochem. 27 1, 125 8– 126 5 (20 04) Ó FEBS 20 04 doi:10.1111/j.14 32- 1033 .20 04.04 026 .x the model further confirmed by determination of the X-ray structure of Staphylococcus ... b-content. On the basis of these observations, we propose that the C-terminal domain (84–1 02) protects the interface between monomers and thus coul...

Ngày tải lên: 19/02/2014, 12:20

... & Nishioka T (20 02) Anal Chem 74, 22 33 22 39. N. Ohtani et al. A fusion protein consisting of RNase H and APase FEBS Journal 27 2 (20 05) 28 28 28 37 ª 20 05 FEBS 28 37 The SCO 229 9 gene from Streptomyces ... complementation of the E. coli RNase H-deficient mutant MIC2067(DE3). N. Ohtani et al. A fusion protein consisting of RNase H and APase FEBS Journal 27 2 (20 05) 2...

Ngày tải lên: 19/02/2014, 18:20

... displayed in the center of the screen till a vocal response triggered the microphone or for a maximum duration of 20 00 msec. Pronunciation errors, hesitations and triggering of the microphone by ... abandon the idea of all-or- none rules which was a central theoretical assumption of these models compared to connectionist ones. As the DRC model is largely based...

Ngày tải lên: 20/02/2014, 19:20

... regard, the best combination of the two subunits that match the smallest molecular mass is a 2 b 2 . Overnight incubation of the protein in this detergent led to a decrease of the area of the first ... in the EPR of the native enzyme (see Introduction) disappeared following the incubation in this detergent. This phenomenon was attributed to the denaturation ac...

Ngày tải lên: 21/02/2014, 00:20

... actions that violate the constraints or the task. Host of these errors occur on the side away frm their current "point st view", even theuah their point of view changes From one ... subjects often switch point of view so that the violation condition is now on the same side a• the subjects' point of view. We see the point of view indicated...

Ngày tải lên: 21/02/2014, 20:20