Báo cáo khoa học: Conformational stability of 17b-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus pptx

... hallmark of the MT structures is the dependence on the sequential position of the cysteine in the primary sequence, the identity of the coordinated metal and its c oordination number [14,48]. The ... isible in the T OCSY with the exception of the side c hain of Cys5 (assigned from the N OESY), and a ll resonances of Gly1 (not identified i n either experime...

Ngày tải lên: 23/03/2014, 13:20

... 8.5, we studied thermal stability of 17b-HSDcl from CD and UV melting curves, from which the temperature of denaturation (T d ) and the van’t Hoff enthalpy of denaturation (DH° vH )of1 7b-HSDcl were ... guanidine hydrochloride; 17b-HSDcl, 17b-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus; DH° vH , van’t Hoff enthalpy of denaturation; SDR, sh...

Ngày tải lên: 16/03/2014, 13:20

... by proteases. The limited proteolysis pattern also led to the establishment that no other potential cleavage sites of apoMb undergo hydrolysis, as a consequence of the stability of the helical fold of the ... etc. are the helix segments [12]. The folding scheme confirms the absence of a stably folded helix F in native apoMb, in contrast with the N state of the...

Ngày tải lên: 30/03/2014, 01:20

... ⁄ v) PAA, the activity of the immobilisates was only $10% of the activity that had been expected from the amount of enzyme introduced into the matrix. This was in accord- ance with the many reports ... velocity of the reac- tion, v max the maximum reaction rate, [x] the concentration of the two substrates, K Mx the Michaelis–Menten constant of substrate x,...

Ngày tải lên: 16/03/2014, 13:20

... time of 1.4 ms caused a significant reduction in the intensity of resonance bR1 (Fig. 3). Other resonances were unaffec- ted within the limits of the signal-to-noise ratio of the spectrum. The ... 3D structure of the N-state [7] T170 faces the cytoplasmic channel at the level of D96. Therefore, a cysteine sub- stitution would alter the hydrophobic pocket and the...

Ngày tải lên: 23/03/2014, 13:20

... term. 3. There is a definition or explanation of the term. 4. There are several technical terms that are re- lated to the term. We have implemented the checking program of the first two conditions in the ... number of pages that sat- isfy a given query. In case the query is a term, its hit is the number of pages that contain the term on the Web. We use the following...

Ngày tải lên: 20/02/2014, 16:20

... the finding that at pH 10 and 75 °C, the inactivation of b-glucosidase also does not involve any loss of secondary structure [1]. Conclusions The stability of the b-glucosidase from the hyperther- mophile ... b-sheet aggregate [21]. The thermal stability of b-glucosidase was assessed by plotting the temperature dependence of the peak intensity at 1618 cm )1 (Fig...

Ngày tải lên: 07/03/2014, 03:20

... located on the top of the substrate-binding site in the nuclease domain of the other subunit, which implies that it may participate in the binding with the poly(A) substrate [14]. In the primary ... increase occurred in the size of the protein. Meanwhile, the peak area of the eluted proteins in the SEC profile was greatly reduced compared with that of the na...

Ngày tải lên: 23/03/2014, 04:21

... [54]. Functional stability of Ngb The conformational stability of the apoNgb structure in acid is in agreement with the enhanced functional acid stability of the holoprotein. In fact, Ngb retains the ability ... we have shown that the acid stability of the Ngb fold must be ascribed to the intrinsic stability of the apoprotein polypeptide chain and is no...

Ngày tải lên: 07/03/2014, 02:20

... formation, which leads to the movement of the charged groups of the protein in order to keep themselves neutral. As a means of testing the role of the surfactant on the protein conformations, ... content. The radius of the volume element [R i ¼ (3V i / 4pN a ) 1/3 ] gives a measure of the proximity of the quencher molecule to the fluorophore. The calculate...

Ngày tải lên: 07/03/2014, 15:20