Báo cáo khoa học: Template-assisted rational design of peptide inhibitors of furin using the lysine fragment of the mung bean trypsin inhibitor pptx

... Template-assisted rational design of peptide inhibitors of furin using the lysine fragment of the mung bean trypsin inhibitor Hu Tao 1,2, *, Zhen Zhang 2, *, ... sequences of the previously synthesized Lys fragment [24] and its mutants studied in this work. (B,C) The topologic structures of the M 4 variant of the mung bean trypsin inh...

Ngày tải lên: 16/03/2014, 13:20

... provided by the annotator, and encodes much of the information needed by the next stage (case-frame linking). There are four tag-values used - "consequence" (the move is a result of the ... current standards. The program could play a game of "noughts-and-crosses" with a user, then produce a fluent sunmmry of what had happened during the game(whet...

Ngày tải lên: 17/03/2014, 19:21

... for Cav-tRNA Arg even in the absence of either enzyme. In contrast, the half life of Arg-tRNA Arg is 46 min. Addition of arginyl-tRNA synthetase from jack bean does not further decrease the stability of the canavany- lated ... from the sequences of the tRNA Arg ACG genes from jack bean and soybean were arginylated to only 6–10% of the theoretical acceptance by t...

Ngày tải lên: 18/02/2014, 13:20

... in any of the EREs, irrespective of the absence and presence of E2. Binding of ERa and ERb was increased in both ERE1 and ERE2 of the HOXC13 promoter (Fig. 5A, lanes 1–4). The levels of E2-induced ... possibili- ties. One of the possibilities is that, even if there is an ERE, it may not be responsive (not participating in the activation) all of the time, probably...

Ngày tải lên: 18/02/2014, 14:20

... were designed on the basis of the conserved regions, and were used for RT-PCR of first-strand cDNA prepared from the Octopus heart. blast searches of the PCR product sequence showed a high level of ... and the importance of C-terminal Arg-NH 2 in the specific recognition of TKRPs by TKRPR is conserved through evolutionary lineages of Octopus. Southern blotting of RT-...

Ngày tải lên: 19/02/2014, 00:20

... 1.10.3.1), but the differentiation between these two types of enzyme is not clear [4]. Looking at the sequences of the two enzymes, both show absolute conservation of the histi- dine residues of the CuA ... be part of the defense sys- tem against wounding and infection by the pathogen [16]. Thus, the timing and location of melanization seems to be essential for th...

Ngày tải lên: 19/02/2014, 07:20

... meth- ylamine are bound to the enzyme in this order, and N-methyl-l-alanine and then NADP + are released from the enzyme. Therefore, the kinetic mechanism of the enzyme is the same as those of conventional ... IV pili [7]. The synthesis of these N-methylated peptides is catalyzed by specific N-methyltransferases such as enniatin synthetase [8] and PilD [9] using S-adenosyl-...

Ngày tải lên: 19/02/2014, 16:20

... work of Hu and Liu (2004a) by mapping the extracted features into a hierarchy of features which de- scribes the entity of interest. M. Gamon et al. (2005) clusters sentences in reviews, then ... are usually the same. For example, “dimension” and “size” are different terms, but they share the same unit “mm” or “inch”. Naturally, a product can be viewed as a tree of features. T...

Ngày tải lên: 20/02/2014, 09:20

... improving the understanding of the recognition results. On the other hand, there were four level-down cases. Three of these cases were caused by the misdetection of errors in the SSC procedure. The ... Preparation of Error-Patterns: As the threshold value for the frequency of the occurrence, we employed a value of not less than 2, therefore we obtained 629...

Ngày tải lên: 20/02/2014, 18:20

... correspond- ing to the largest eigenvalue) is the direction in which the sum of the square of the projections of the row vectors of the matrix is maximum. In fact, the principal eigenvector of a graph ... three taken together. The individual contribution of the other eigenvalues to the spectrum is significantly lower than that of the top three. Third, the...

Ngày tải lên: 22/02/2014, 02:20