... 271) 705encoding the DNA-binding domain of HSF, whereas cloneC2 contained an insert of 2771 nucleotides including introns and an ORF encoding 513 amino acids.By using 5¢ -and3 ¢-RACE, the full-length ... (a3 and b3) and two heterotrimers (a2b1 and a1b2). The existence of the four types of trimer may be reflected in the broadband migrating at 200 kDa in Fig. 7. On the other hand,a band ... cycle of 15 min at 95 °C,amplification cycles of 0.5 min at 94 °C, 0.5 min at 50 °C and 0.5 min at 72 °C, and one termination cycle of 1 min at72 °C, with 35 cycles in total for HSF1a and HSF1b and...