Báo cáo khoa học: Separation and characterization of caveolae subclasses in the plasma membrane of primary adipocytes; segregation of specific proteins and functions docx

... in the HD -caveolae and LD -caveolae; (c) the VHD -caveolae contained almost a third of the plasma membrane caveolin, and the majority of cellu- lar caveolin is found in the plasma membrane of adipocytes; ... 2006 FEBS Separation and characterization of caveolae subclasses in the plasma membrane of primary adipocytes; segregation...

Ngày tải lên: 16/03/2014, 13:20

... Taken together with the inability of P III and P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the bind- ing and uptake of these two isoforms does ... in the B-chains, P III has only eight of these and lacks Cys506, indicating that it must lack one of the usual four intra-chain disulfide bridges. The primary s...

Ngày tải lên: 18/02/2014, 16:20

... coincide with the N-terminus of the intracellular enzyme (Fig. 1). The segments in the N-terminal portion around the copper ligand amino acids of the active site are well conserved between the ... determining enzyme activity on 15 mml-dopa in the presence of the inhibitors. The concentration of the inhibitor was 0.1, 1, 10 or 100 mm, depending on the in-...

Ngày tải lên: 19/02/2014, 06:20

... calcium binding domains (EF1–4) and calcineurin A binding domain are shown in the box. The dot in the EF1 box repre- sents the modified amino acids alanine (A) as compared to the oxygen containing-calcium ... [7,8]. CBL proteins contain four Ca 2+ -binding EF hand motifs [9] and functions by interacting and regulating a group of Ser ⁄ Thr pro- tein kinases called CBL-...

Ngày tải lên: 19/02/2014, 07:20

... 271) 705 encoding the DNA-binding domain of HSF, whereas clone C2 contained an insert of 2771 nucleotides including introns and an ORF encoding 513 amino acids. By using 5¢ -and3 ¢-RACE, the full-length ... (a 3 and b 3 ) and two heterotrimers (a 2 b 1 and a 1 b 2 ). The existence of the four types of trimer may be reflected in the broad band migrating at  200 kDa...

Ngày tải lên: 19/02/2014, 12:20

... low-capacity process. These findings suggest that the conformational change of the carrier protein following H + binding and substrate binding represents the rate limiting step in the substrate translocation ... concentration of 3 mm inhibited the uptake of the tracer Bip-[ 3 H]Pro (4 nm) during 30 min of incubation by only 21% (data not shown). We con- clude that the...

Ngày tải lên: 07/03/2014, 05:20

... some of the catalytic activity lost by the impo- sition of the Q143A substitution. These mutations are subsequently kinetically characterized separately and in combination in the context of the ... mutagenesis, and the parent Q143A hMn- SOD and wild-type hMnSOD, are given in Table 2 and Fig. 1. Catalysis of the decay of superoxide by the single mutants...

Ngày tải lên: 07/03/2014, 11:20

... (Met153 of the ML1p and ML2p B-chains and Met156 of the ML3p B-chain corresponding to the ricin B-chain Leu152; and Met234 of the ML1p and ML2p B-chains corresponding to the ricin B-chain Leu233). Estimation ... Asn255 and Gln256 of the ricin B-chain in the second sugar binding site [28]. The exceptions are the replacement of Trp38 fi Ser in the ML2p...

Ngày tải lên: 07/03/2014, 15:20

... vector following the manufacturer’s instruction (Gateway, Invi- trogen). The correct insertion of the Rv1399c ORF was confirmed by DNA sequencing. In our hands, protein expression using the commercially available ... followed by Lac O (the DNA binding site of Lac I). The resulting construct constitutively expresses Lac I, which inhibits the T7 RNA polymerase in b inding to...

Ngày tải lên: 07/03/2014, 16:20

... the reducing equivalents of NADH are held in the nicotinamide ring on the re-side of the flavin isoalloxazine ring with the C4 position of the nicotina- mide ring located 3.49 A ˚ from N5 of the ... from the spectrum the fate of the reduced pyr- idine nucleotide. During the addition of the first equiv- alent of NADPH, there is a blue shift in the 380...

Ngày tải lên: 07/03/2014, 17:20