Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot

... demonstrated the transport of conjugated and unconjugated lipophilic anions by MRP2. The absence of MRP2 from the canalicular membrane of human hepatocytes is the molecular basis of the Dubin–Johnson ... identification of apical localization signals in the C-termini of CFTR [51] and of the type IIb Na + /P i co-transporter [53]. In contrast to CFTR [51] and t...

Ngày tải lên: 31/03/2014, 09:20

... consists of three domains: an N -domain and two ATP-binding domains referred to as the D1 and D2 domains. Interestingly, deletion of the N -domain from ClpA not only abol- ishes binding of the adaptor ... interaction between ClpS and the N -domain of RR ⁄ AA, and this result suggests that neither the local nor the overall structure of the RR ⁄ AA mutant was...

Ngày tải lên: 07/03/2014, 05:20

... locate the catalytic center and predict the shape of the dimeric protein. The model reinforced our hypothesis proposed above. To further address this hypothesis, we have characterized the protein ... after the first transaldimination are rate-limiting in the entire catalytic reaction of mammalian HDC [19]. On the other hand, the crystal structure of DDC has shown...

Ngày tải lên: 08/03/2014, 08:20

... this study we used IR spectroscopy to study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the ... 3273–3286 ª 2006 The Authors Journal compilation ª 2006 FEBS Structural study of the catalytic domain of PKCf using infrared spectrosco...

Ngày tải lên: 16/03/2014, 13:20

... [27,35] and other members of the papain family, and is made up of two domains, L and R, with a V-shaped active site cleft at the interface of the domains (Fig. 1). Amino acid sequence of ervatamin-A ... erva- tamin-A, the P3 moiety of both the molecules of the asymmetric unit runs along the extended backbone of residues 65–64 and is partly exposed to the...

Ngày tải lên: 18/02/2014, 16:20

... and part of the connection domains of retroviral RTs, and appears to have a similar structure to these enzymes [10,13]. The RT domain and domain X are required for RNA splicing [12]. The En domain, ... site in subdomain DIVa of the intron, whereas other regions of the RT and domain X interact with con- served catalytic core regions [12]. Domain X is locate...

Ngày tải lên: 15/03/2014, 09:20

... contains three helices (a1–a3) and one b-strand (A) parallel to the first b-strand, B, of the jelly roll core. The linkage of the fourth (E) and fifth (F) b-strand of the jelly roll fold is built ... VioC•L-arginine•tar- trate•Fe(II). The b-strands B, G, D, I and C build the major side of the jelly roll fold, and the minor side is built by the b-strands F, E...

Ngày tải lên: 16/03/2014, 02:20

... works 4453 of the S1-bound chloride anion and ‘pushes’ the Cl ) out of the bottom of the S1 pocket (the occupancy of the Cl ) in this structure is 0.7). The alternate confor- mation of the Asp443 ... with the catalytic Zn1 and co -catalytic Zn2 displaying respective occupancies of 0.80–0.95 and 0.45–0.80. The lower occupancy of the co -catalytic Zn...

Ngày tải lên: 23/03/2014, 05:22

... in the )1 subsite of PAL is energetically favorable and contri- butes significantly to the higher affinity of PAL for the pentasaccharide M592 (Table 3). In the case of con A on the other hand, ... Gly104 of PAL which is Thr226 in con A. The side chain of Thr226 occupies the space taken by the conserved waters 116 and 117 of PAL and makes a hydrogen bond to O3...

Ngày tải lên: 23/03/2014, 11:20

... both mesophilic. Hence, the mean of the distribution is, by definition, null. The mean and the standard deviation (Eqns 1–3) of the distribution of the dif- ferences of the structural properties ... percentages of the overall surface composing the interface and the core inter- face, the percentages of polar and nonpolar atomic contri- butions to the...

Ngày tải lên: 30/03/2014, 03:20