Báo cáo khoa học: Characterization of SCP-2 from Euphorbia lagascae reveals that a single Leu/Met exchange enhances sterol transfer activity pdf

... (5¢-AAGTCCCAAAATATTATGGA TATGATGGCTCGATTTCTCGAG-3¢); ELE28 (5¢-ATG AAAGTTCAAGAGAAGATCAATCTC-3¢); ELM99 (5¢- ATGAGGGGTGCTATGAAGATCAAGG-3¢); ATL100 (5¢-ATTAGGGGTGCGCTGAAGATCAAGG-3¢); ATL14- L15F18 (5¢-AAATCCGATGCAATCCTGGACCTGCTG AAGGAATTTCTTTCCACCGACGCC-3¢). ... Authors Journal compilation ª 2006 FEBS 5655 Characterization of SCP-2 from Euphorbia lagascae reveals that a singl...

Ngày tải lên: 16/03/2014, 12:20

... Oliveira FW, Chavante SF, Santos EA, Dietrich CP & Nader HB (1994) Appearance and fate of a beta- galactanase, alpha, beta-galactosidases, heparan sulfate and chondroitin sulfate degrading ... appropriate material for the study of intact CS chains. Hexasaccharides Although data from disaccharides, trisaccharides and tetrasaccharides are valuable for the detailed structural charact...

Ngày tải lên: 16/03/2014, 14:20

... cells from human adenocarcinomas Anna Maria Luciani 1 , Sveva Grande 1 , Alessandra Palma 1 , Antonella Rosi 1 , Claudio Giovannini 2 , Orazio Sapora 3 , Vincenza Viti 1 and Laura Guidoni 1 1 Dipartimento ... arachidonic acid chains, and at 0.93–2.04 p.p.m., attributed to linolenic acid chains on the basis of a comparison with lipid extracts and from the data available in the literatu...

Ngày tải lên: 18/02/2014, 13:20

... 5¢-CTTTAACTTGTTGGGCACTGG CATTG-3¢; NP6, 5¢-TTGATCGATTCTGTCTATGCCC CA-3¢ along with the adaptor primers: AP1 (5¢-GTAATAC GACTCACTATAGGGC-3¢) and AP2 (5¢-ACTATAGGG CACGCGTGGT-3¢). Nested PCR was carried ... obtain the full length sequence: OP5, 5¢-GACTGTAACGGTCATGGYACMAYGT-3¢; OP6, 5¢-GATGAAAATCCTAACCTCTCCCCCGCACAG- 3¢; OP7, 5¢-ACTGCACCTACGGCGGGTCGTTGGTACG TG-3¢; NP4, 5¢-GACACCGTAGGTTGAGCCGCCAA...

Ngày tải lên: 19/02/2014, 07:20

... His 6 -PecA, His 6 -PecE, and His 6 -PecF His-tagged PecA, E and F were purified separately by metal ion chelating affinity chromatography on chelating seph- arose (fast flow; Amersham Pharmacia Biotech AB, according ... the activity at 45 °C being 20% less. Apparently, the lyase has a high transient at 45 °C, but also becomes more rapidly inactivated than at 37 °C, probably by precipitation...

Ngày tải lên: 21/02/2014, 03:20

... 5¢-GAGCCCGGATCCACCATGAA GGTCTCAATAATT 3¢;3¢ primer, 5¢-CTGACG GAAT TCTTAAACATTAATGCC 3¢. These primers encoded a Kozak consensus sequence as well as BamHI and EcoRI restriction sites. The PCR-amplified ... observed with M. brassicae CSPMbraA6 [32]. We observed also that BrC15-Ac was able to displace ASA, suggesting that brominated fatty acid and ASA both associated with W81 in the same l...

Ngày tải lên: 21/02/2014, 03:20

... Journal compilation ª 2011 FEBS 1853 Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae Malea M. Kneen 1 , Razvan Stan 1 , Alejandra Yep 2 , ... orders of magnitude. When viewed as a whole, it is apparent that these mutations have had the most beneficial effect on the decarboxylation of 2-ketopentanoic acid. Each varia...

Ngày tải lên: 06/03/2014, 00:21

... shown). An alternative explanation is that membrane PE is attached to membranes through a hydrophobic anchor that has been added to the protein post-translationally. A web-based (expasy) analysis of ... the membranes. Accordingly, we found that a considerable amount of PE activity bound to the membranes was released upon a 0.5 m NaCl wash of total membrane preparation (T...

Ngày tải lên: 07/03/2014, 06:20

... Gui- maraes PE, Ojopi EP, Paquola AC, Piazza JP, Nishiy- ama MY Jr, Kitajima JP, Adamson RE et al. (2003) Transcriptome analysis of the acoelomate human para- site Schistosoma mansoni. Nat Genet ... further indebted to Ana Marı ´ a Ferreira (Ca ´ tedra de Inmunologı ´ a, University of Uruguay) and her collabo- rators for experimental data that helped us optimize metabolic labelling i...

Ngày tải lên: 07/03/2014, 12:20

... intriguing that the order of addition of tRNA and SF to the activity assay affected the tRNA- saturation curve. This suggested that SF might form a complex with unacylated tRNA in solution that has higher ... also be mentioned that it has been suggested that the 50S sub- unit may contain a domain that senses the aminoacyla- tion state of the tRNA in analogy with the T-...

Ngày tải lên: 07/03/2014, 16:20