Báo cáo khoa học: Inactivation of tyrosine phenol-lyase by Pictet–Spengler reaction and alleviation by T15A mutation on intertwined N-terminal arm docx

... compilation ª 2006 FEBS 5573 Inactivation of tyrosine phenol-lyase by Pictet–Spengler reaction and alleviation by T15A mutation on intertwined N-terminal arm Seung-Goo Lee 1 , Seung-Pyo Hong 2 , ... cofactor through a con- densation reaction with l-DOPA. Stabilization of cofactor binding by T15A mutation The extended lifetime of T15A in 10 mml-DOP...

Ngày tải lên: 16/03/2014, 12:20

... participated in the reaction is not certain. On one hand, the decay of the radical to the broad band at 350 nm formed isosbestic points along a large part of the reaction [Fig. 3]. On the other hand, BSAO ... TPQ. The final band intensity matched the residual activity of the solution. The reaction was slow, implying previous reoxidation of active mole- cules by oxygen and...

Ngày tải lên: 19/02/2014, 12:20

... liposome aggregation at all the AIIt concentrations and all the Ca 2+ concentrations tested (Figs 3 and 4). At a higher concen- tration of AIIt (10 lg) less inhibition was observed. Effect of GSNO on AIIt-mediated ... physiological processes and thus modifies their functions. Interaction of NO with the heme or nonheme iron of proteins leads to activation of soluble guanylyl c...

Ngày tải lên: 08/03/2014, 10:20

... proton removal. Both mutations result in a complete loss of tetrahydrofolate-dependent and tetrahydrofolate-independent activi- ties. The mutation of Y51 to F strongly affects the binding of pyridoxal 5¢-phosphate, ... The plane of the pyridine ring is rotated by about 22° along the C2–N1 axis. As a consequence, the C5A atom of PLP moves by 1.66 A ˚ . The change in the orien...

Ngày tải lên: 16/03/2014, 06:20

... to activation of protein kinase B and inactivation of GSK-3. We demonstrate in this study that inactivation of phosphorylase but not inhibition of GSK-3 mimics insulin stimulation of glycogen ... phos- phorylase inactivation cannot be explained by either inhi- bition of glycogen degradation or activation of glycogen synthase alone and suggests an additional role for...

Ngày tải lên: 31/03/2014, 01:20

... administration of native CT or CT-B, and evaluated by western blotting for their content of ARF and ARF-6 using their respective polyclonal and monoclonal anti- bodies. Arrows to the right of each ... acidification rates were unaffected by diphtheria toxin and free CT-B, which confirmed the potential role of cAMP in mediating the effects of CT on endosome acidification [1...

Ngày tải lên: 19/02/2014, 02:20

... degrades on out -of- domain data, and Twitter poses additional challenges due to the conversational nature of the text, the lack of conventional orthography, and 140- character limit of each message ... algorithm (Philips, 1990) 9 to create a coarse phonetic normalization of words to simpler keys. Metaphone consists of 19 rules that rewrite consonants and delete vowels. For...

Ngày tải lên: 20/02/2014, 04:20

... volume of 100 lL. After pre- incubation at 30 °C for 5 min, the reaction was initiated by the addition of 10 lL of enzyme solution (0.83 mgÆmL )1 ). After 3 min, the reaction was stopped by the ... catabolism of Escherichia coli: involvement of alsI and regulation of als regulon expression by allose and ribose. J Bacteriol 181, 7126–7130. 12 Kim C, Song S & Park...

Ngày tải lên: 15/03/2014, 00:20

... works Improvement of a monopartite ecdysone receptor gene switch and demonstration of its utility in regulation of transgene expression in plants Venkata S. Tavva 1,2 , Subba R. Palli 1 , Randy D. Dinkins 3 and ... the expression of AtZFP11 and that the phenotype of AtZFP11 could be induced by the application of ligand. In addition, the affected plants recovered after with...

Ngày tải lên: 16/03/2014, 06:20

... 2.65 kb of cloned genomic DNA sequence containing the 5¢- part of exon 2, intron 1, exon 1 and its 5¢-flanking noncoding sequence, and 2.7 kb of sequence containing the 3 ¢-part of exon 5 and 3¢-flanking ... 3¢-end of exon 5 and its substitution with a reporter (b-galactosidase) and selection marker (neomycin resistance). This vector was comprised of left and right homol...

Ngày tải lên: 16/03/2014, 18:20