... preferential hydration of the protein domain is the driving force of protein stabiliza-tion or folding [3,4]. Furthermore, the effect of osmolytes on the functional activity of an enzyme hasalso ... published elsewhere. We havetherefore measured the thermodynamic parameters of RNase-A in the presence of these amino acids and amino acid derivatives, and values of DGD°, measuredin triplicate, ... complexes [29]. On the other hand,kcat⁄ Kmis an apparent second-order rate constant thatrefers to the properties and the reaction of the freeenzyme and free substrate [29]. We have therefore esti-mated...