... Bacterial IscU is a well folded and functional single domain proteinSalvatore Adinolfi1, Francesca Rizzo1, Laura Masino1, Margie Nair1, Stephen R. Martin1, Annalisa Pastore1 and ... uniformly15NlabeledsampleofE. coli IscU; (B) as forsample A, after addition of Fe(II) in a Fe(II)/IscU ratio of 2 : 1;(C) same as sample A with a ratio Fe(II)/IscU of 5 : 1 after partialoxidation by atmospheric ... 8.0, 50 mMNaCl and 10 mM2-mercaptoethanol. Lower panel: Experimental absorbance at 280 nmas a function of the radial position and data fitting to the equationreported in Materials and methods....