Báo cáo khoa học: Novel a-conotoxins from Conus spurius and the a-conotoxin EI share high-affinity potentiation and low-affinity inhibition of nicotinic acetylcholine receptors doc

... heterogeneous group of peptides with respect to their primary and secondary structures, their antimicrobial potentials, their effects on host cells, and the regulation of their expression. Most ... on their capacity to induce the production of proinflammatory cytokines, to pro- mote the recruitment of dendritic cells and monocytes to the site of injury, and to enhance...

Ngày tải lên: 18/02/2014, 06:20

... family proteins, have been described to promote the transfer of SUMO from E2 to target proteins in vivo. The attachment of SUMO may result in diverse effects on the substrate protein, including ... substrates, and modulate the function of several transcription factors [31]. LRH-1 is a target protein of SUMO modification [32,33]. The hinge region lysine, residue 289 in th...

Ngày tải lên: 23/03/2014, 06:20

... determination and cloning of the cDNA encoding two novel peptides, SrIA and SrIB, found in the venom of Conus spurius. The pattern and the spa- cing of their cysteines indicate that they belong to the a4 ... aA families. On the basis of the number of residues between the sec- ond and third cysteines and on the spacing between the third and fourth...

Ngày tải lên: 16/03/2014, 11:20

... Gla(3)–TxVI: confirmation of the presence of BrTrp. Product ion mass spectrum of the doubly charged ion at m ⁄ z ¼ 660.18. The isotopic distribution of the b 2 ion (inset) indicates the presence of bromine. The MS ... studies utilizing the Conus c-carboxylase and synthetic peptide sub- strates localized the c-CRS of Gla(1)–TxVI to the region )14 to )1 of th...

Ngày tải lên: 23/03/2014, 11:20

... clinical applications of cathelicidins is their haemolysation of mammalian cells. However, the good thing is that the dose of cathelicidin resulting in haemolysis is often much higher than the MIC. The haemolytic ... Among them, the lung cDNA library was of the best quality, from which some posi- tive clones containing an insert of 555 bp were identi- fied and isolate...

Ngày tải lên: 22/03/2014, 21:21

... If so, the function of these b-glucan oligomers for the fungus might be very different from that of the known cell-wall glucans. Clarifying the origin of these oligosaccharides and their function ... different defense pathways: acidic and basic PR3 encoding chi- tinase enzymes of the PR protein family, PAL and CCoAOMT from the phenylpropanoid pathway, and STC...

Ngày tải lên: 23/03/2014, 11:20

... this step. The antibacterial activity of gel extracts from the 6 m urea ⁄ PAGE was analyzed using the growth -inhibition plate method, and the molecular mass of the antibacterial protein was confirmed ... molecular mass of the antibacterial protein. Antibacte- rial fractions correspond to the upper diagram and are indicated by ‘+’. The asterisk indicates the spec...

Ngày tải lên: 29/03/2014, 08:20

... matching parts of the sense and antisense strands of the DNA sequence were used. The synthesized fragment was inserted between the BamHI and XhoI sites of pGEX-6P-1. Nucleotide sequences of the recombinant ... [8–13], the structure of the C-terminal nontoxic region has not yet been determined. Therefore, the crystal structure of Cry protoxin is still unknown....

Ngày tải lên: 29/03/2014, 09:20

... sequence, the typ- ical HSE contains at least three nGAAn units [4]. The helical repeat of the DNA (10.5 bp), the length of the unit (5 bp) and the inverted nature of the repeats, posi- tions all of the ... DBD– HSE co-crystals, the protein–protein interface consists of the helix 2 amino-terminus, the turn and the wing [15]. Unlike other winged helix-turn...

Ngày tải lên: 18/02/2014, 04:20

... TNSALP (1559delT) being segregated into COP II vesicles at the exit site from ER and therefore the mutant protein remains longer in the lumen of the ER and finally is diverted to the degradation ... oligosaccharide chains of glycoproteins [31]. The involvement of EDEM and ⁄ or SCF Fbs2 in the degrada- tion of TNSALP mutant proteins is currently being investigated....

Ngày tải lên: 19/02/2014, 17:20