Báo cáo khoa học: UXT interacts with the transcriptional repressor protein EVI1 and suppresses cell transformation ppt
Báo cáo khoa học: CD91 interacts with mannan-binding lectin (MBL) through the MBL-associated serine protease-binding site doc
... [12–15].SP-A and SP-D are lung surfactant proteins. LikeMBL and the ficolins they are pattern-recognitionmolecules but do not share the property of binding the MASPs and therefore do not activate the ... N-acetylated and neutralcarbohydrates [8].It is synthesized in the liver and, like the other ficolins, has the potential of initiating the MBL ⁄ lectin complement cascade through the MASPs [12–15].SP-A ... of the binding sites on C1q for CD91were shared with calreticulin [19].Here, it is shown that CD91 interacts directly with MBL and, to a lesser extent, with L-ficolin and there-fore has the...
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Báo cáo khoa học: NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expression in the neural tube pptx
... (Zeiss).RESULTSNBR1 interacts with the PKC zeta interacting protein FEZ1 and the calcium and integrin binding protein CIBIn order to identify proteins that interact with NBR1, ayeast two-hybrid ... shown the accumulation in the nucleus and the cytoplasm of both t ransfected and endogenous CIB protein [19]. The presence of CIB in different cellular c ompartments, and its ability to interact with ... encoded the full-lengthcDNA of the calcium and integrin binding protein (CIB) inframe with the GA L4 AD.Mapping of regions involved in the interactionshow that CIB and FEZ1 bind in the same...
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Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
... of free and bound unlabeled C160–RepA and wild-type RepA, with the protein concentration con-stant. The ratio of the apparent FW94of bound and freeRepA was then used to estimate the fraction ... on the orientation of the AEDANS absorp-tion and emission transition dipoles with respect to the protein axes. For a case where any of these are parallel to the long axis, the value takes the ... 0.7 and 1.3 times the value ofR(2/3), presented in Table 2.Nevertheless, the R(2/3) value in the complex with 1DR is in excellent agreement with the distance mea-sured between the...
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Báo cáo khoa học: SHIP2 interaction with the cytoskeletal protein Vinexin ppt
... 200 dilution. The cells were then washed three times with NaCl ⁄ Pifor 10 min and mounted with the SlowFade lightantifade kit (Molecular Probes, Leiden, the Netherlands)following the manufacturer’s ... transfected cells and MEF cells. Stimulation of the cells with EGF did notmodulate the association of the two proteins althoughSHIP2 tyrosine phosphorylation was increased. This isin agreement with ... unstimulated and EGF-stimulated cells (data not shown).Endogenous Vinexin a and b associate with SHIP2 in MEF cellsMEF cells express both Vinexin a and b (Fig. 3A,C).They were used to further confirm the...
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Báo cáo khoa học: Nucling interacts with nuclear factor-jB, regulating its cellular distribution potx
... Bcl-3,as well as p105 and p100. In unstimulated cells, NF-jBforms a complex with the IjBs and is thereby lockedinto the cytoplasm. IjBa is the best-characterized IjB, and is also a target of ... importantfor the regulation of the distinct intracellulardistribution of NF-jB in its resting and activatedstates.Materials and methods Cell lines and culture conditionsHEK293, MEF and COS7 cells ... blocking, the cells were incubated with 3%bovine serum albumin in NaCl/Pifor 1 h. Then, they wereincubated with the anti-HA (3F10) or NF-jB-p65 antibody(F-6) overnight at room temperature, and the...
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Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf
... ATnI130)252, and ATnI232)292did not migrate into the gels, while free ATnI-52K and ATnI1)128exhibited a band near the origin and at the middle of the gel, respectively. The bands corresponding to the ... SDS ⁄ PAGE. Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in the presence of bothTnC and Ca2+. Ac, actin; ... interact with the N- and C-domains, respectively, of TnC [18,19]. The inhibitory region is known to interact with both the N- and C-domains, but preferentially with the C-domain [18,20,31]. In the...
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Tài liệu Báo cáo khoa học: Solution structure of the matrix attachment region-binding domain of chicken MeCP2 ppt
... with anantiparallel one-turn helix on the other face. It is thoughtthat the two inner strands of the b-sheet lie within the majorgroove of the DNA and that a hydrophobic pocket formedby the ... calculated for the best 10 structures with respect to the value of the target function. Analysis of the / and w anglesof the nonglycine and nonproline residues was carried out with the computer ... P153 and G162 are buried in the protein core. Also, F156, F158, and V160 are tightly packed into the hydrophobic core of the domain. On the other hand,residues D152, N154, D155, D157, and R163...
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Báo cáo khoa học: NF-jB regulates the transcription of protein tyrosine kinase Tec pdf
... co-transfected without or with pcDNA1-p65 into Namalwa cells by electroporation, and cells weretreated 32 h later without or with MG132 (10 lM) for another 16 h.Luciferase activity was measured and the ... induces the human Tec promoter in these cells (1.7-fold), and that the proteasome inhibitor MG132 significantly reduced the transcriptional activity (Fig. 6). Similar results wereobtained in the cell ... presentation and apoptosis[49,50]. The ubiquitin proteasome pathway controlskey cellular and biological processes and thereforeplays crucial roles in health and disease, and there isincreasing...
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
... comparison, the sequence encoding exon I in the human and mouseMGP genes is split into two exons (IA and IB) in the X. laevis gene, with the site of the intron insertion localizedwithin the 5¢ UTR ... xMGP gene and other known MGP genes (mouse and human) indicates that all other introns (2, 3 and 4) arelocated at conserved sites within the MGP coding sequence(Fig. 2 ). Analysis of the phase ... examined the effects of extracellular Ca2+concentrations (1.8, 3.0 and 6.0 mM) on the transcriptional activation of the 3x()70/)36)TATALUC reporter plasmid in A6 cells. Increasingextracellular...
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Báo cáo khoa học: Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris ppt
... concentration) for three successivedays, once in the morning and once in the evening. Protein profiles in the medium and the cell pellet were compared.Proteins in the culture medium were analyzed after ... (syncytium) cell for-mation was recorded microscopically in the CEM cell cul-tures, and the number of giant cells was estimated as the percentage of the number of giant cells present in the non-treated ... methylotrophic yeast Pichia pasto-ris with the Saccharomyces a-factor sequence to direct the recombinant protein into the secretory pathway and express the protein into the med-ium. Approximately 12...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ