Báo cáo khoa học: UXT interacts with the transcriptional repressor protein EVI1 and suppresses cell transformation ppt

... [12–15]. SP-A and SP-D are lung surfactant proteins. Like MBL and the ficolins they are pattern-recognition molecules but do not share the property of binding the MASPs and therefore do not activate the ... N-acetylated and neutral carbohydrates [8] . It is synthesized in the liver and, like the other ficolins, has the potential of initiating the MBL ⁄ lectin complemen...

Ngày tải lên: 06/03/2014, 01:23

... (Zeiss). RESULTS NBR1 interacts with the PKC zeta interacting protein FEZ1 and the calcium and integrin binding protein CIB In order to identify proteins that interact with NBR1, a yeast two-hybrid ... shown the accumulation in the nucleus and the cytoplasm of both t ransfected and endogenous CIB protein [19]. The presence of CIB in different cellular c ompartm...

Ngày tải lên: 08/03/2014, 10:20

... of free and bound unlabeled C160–RepA and wild-type RepA, with the protein concentration con- stant. The ratio of the apparent F W94 of bound and free RepA was then used to estimate the fraction ... on the orientation of the AEDANS absorp- tion and emission transition dipoles with respect to the protein axes. For a case where any of these are parallel to the lo...

Ngày tải lên: 07/03/2014, 04:20

... 200 dilution. The cells were then washed three times with NaCl ⁄ P i for 10 min and mounted with the SlowFade light antifade kit (Molecular Probes, Leiden, the Netherlands) following the manufacturer’s ... transfected cells and MEF cells. Stimulation of the cells with EGF did not modulate the association of the two proteins although SHIP2 tyrosine phosphorylation was i...

Ngày tải lên: 07/03/2014, 12:20

... Bcl-3, as well as p105 and p100. In unstimulated cells, NF-jB forms a complex with the IjBs and is thereby locked into the cytoplasm. IjBa is the best-characterized IjB, and is also a target of ... important for the regulation of the distinct intracellular distribution of NF-jB in its resting and activated states. Materials and methods Cell lines and culture conditio...

Ngày tải lên: 16/03/2014, 04:20

... ATnI 130)252 , and ATnI 232)292 did not migrate into the gels, while free ATnI-52K and ATnI 1)128 exhibited a band near the origin and at the middle of the gel, respectively. The bands corresponding to the ... SDS ⁄ PAGE. Lanes a and d, in the absence of both TnC and Ca 2+ ; lanes b and e, in the presence of TnC and the absence of Ca 2+ ; lanes c and f, in t...

Ngày tải lên: 20/02/2014, 01:20

... with an antiparallel one-turn helix on the other face. It is thought that the two inner strands of the b-sheet lie within the major groove of the DNA and that a hydrophobic pocket formed by the ... calculated for the best 10 structures with respect to the value of the target function. Analysis of the / and w angles of the nonglycine and nonproline residues was...

Ngày tải lên: 21/02/2014, 00:20

... co-transfected without or with pcDNA1-p65 into Namalwa cells by electroporation, and cells were treated 32 h later without or with MG132 (10 l M) for another 16 h. Luciferase activity was measured and the ... induces the human Tec promoter in these cells (1.7-fold), and that the proteasome inhibitor MG132 significantly reduced the transcriptional activity (Fig. 6). Similar...

Ngày tải lên: 07/03/2014, 00:20

... comparison, the sequence encoding exon I in the human and mouse MGP genes is split into two exons (IA and IB) in the X. laevis gene, with the site of the intron insertion localized within the 5¢ UTR ... xMGP gene and other known MGP genes (mouse and human) indicates that all other introns (2, 3 and 4) are located at conserved sites within the MGP coding sequence (Fig...

Ngày tải lên: 08/03/2014, 10:20

... concentration) for three successive days, once in the morning and once in the evening. Protein profiles in the medium and the cell pellet were compared. Proteins in the culture medium were analyzed after ... (syncytium) cell for- mation was recorded microscopically in the CEM cell cul- tures, and the number of giant cells was estimated as the percentage of the n...

Ngày tải lên: 14/03/2014, 23:20