Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc

... understanding the molecular mechanism of protein association ⁄ dissociation is fun- damental in lead design and synthesis of peptide-based antagonists that aim to bind and sequester proteins that ... this overall value into its component kinetic steps. The findings of this study are therefore of interest to the protein design field in general, but also inform upon how t...

Ngày tải lên: 18/02/2014, 13:20

... that oxidation induced a change in conformation of the intracellular domain of this panel of RPTPs. A functional consequence of the change in confor- mation in the cytoplasmic domain is a change in rota- tional ... required for this effect, suggesting that the epitope tag of one monomer is masked by the ectodomain of the other monomer in the dimer, an...

Ngày tải lên: 18/02/2014, 17:20

... expressed in the eight human cancer cell lines examined, and HO-1 expression is detectable in five of the cell lines, including HeLa cervical cancer and HepG2 hepatoma. Down -regulation of HO-2 expression ... HO-1 and HO-2 in various human cell lines. (A) Total RNA and proteins were prepared from the indicated cell lines and subjected to northern blot analysis and (B) west...

Ngày tải lên: 19/02/2014, 05:20

... enzyme–target binding and the catalysis. K and P denote the free concentrations of kinase and phosphatase, respectively, and L n and Q n are the respective dissociation constants for the kinase–target and ... dephosphorylation by the kinase) due to competi- tion of the two enzymes for the target. Indeed, there is experimental evidence for kinases and phosphatases com...

Ngày tải lên: 07/03/2014, 10:20

... much turmoil regarding the ‘life is sacred at any cost’ maxim [1]. Current technology is capable of indiscriminately maintaining some of the vital functions of the body, but the same technology ... passed the point of diminishing returns. However, there is a continuing trend of surrogates demanding that moribund patients be kept on life support after prevailing medic...

Ngày tải lên: 25/10/2012, 10:45

... little is known about how the initial molecu- lar activity of Sry is translated into cellular structure and organ morphology. Interestingly, Dmrt1, the ancestor of Dmrt 1bY, is one of the downstream ... dmrt 1bY regulation mechanisms could be shown. Tran- scriptional regulation: the feedback autoregulation of dmrt 1bY promoter activity and transregulation by its...

Ngày tải lên: 14/02/2014, 19:20

... carrying human Hsp70 cDNA, and examined the protein levels by western blot analysis with Hsp70 antiserum after 36 h. The effi- ciency of transfection mediated by the Lipofectamine Plus reagent ranged ... lost. Taken together, our results demonstrate an impor- tant relationship among the regulation of nucleo- lin ⁄ C23, the activation of caspase-3 and the induction...

Ngày tải lên: 16/02/2014, 09:20

... miR-23a causes the downregulation of IL6R by binding to its first conserved binding site, which then leads to the development of gastric adeno- carcinoma. Materials and methods Human cancer tissue samples Fresh-frozen ... levels. To confirm the direct regulation of IL6R by miR-23a, we used an EGFP-IL6R 3¢ UTR reporter vector bearing the potential miR-23a binding...

Ngày tải lên: 18/02/2014, 04:20

... suggesting a possible role in the transition between pupae and adult. It is generally believed that gene transcripts induced in vitro only in the presence of 20E and the protein synthesis inhibitor ... processes. In A. aegypti, the discrete expression of both AaHR3 and AaHR4 suggests their function in the regulatory hierarchy in the mosquito fat body dur- ing v...

Ngày tải lên: 18/02/2014, 13:20

... how these toxins modify the cytoskeleton of neuroblastoma cells: (a) the distortion of the actin cytoskeleton begins at early stages, being detectable after 10 min of toxin incubation; and (b) ... starting this type of study with an intestinal model: (a) the great complexity of the actin cytoskeleton in these cells; and (b) the severe gastrointestinal toxicity exerte...

Ngày tải lên: 18/02/2014, 14:20