Báo cáo khoa học: Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin pdf

... Journal 274 (2007) 677–686 ª 2006 The Authors Journal compilation ª 2006 FEBS Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin Joa ˜ o B. Vicente 1 , ... electrons at the site of reaction with NO, this prompted us to use E. coli FlRd as a model to study the kinetics of electron transfer (e...

Ngày tải lên: 16/03/2014, 11:20

... deter- mined, as was the area of each peak. The ratio of the density, which is related to the amount of EF-P bound, to the area of the peaks, which is related to the total amount of the ribosome population ... required to accommodate fMet–tRNA in the P-site of the 70S ribosome. A computer-simulated structure of the 70S ribosome of E. coli was us...

Ngày tải lên: 16/03/2014, 06:20

... After the concentrations of the resultant glucose and fructose were measured, the extent of sucrose splitting was determined from the amount of fructose released, and the extent of glucosyl transfer ... addition, the kinetics of the acceptor reactions appeared to be similar between GS and GSGB. Kinetic simulation of GTF glucan synthesis To explain the tim...

Ngày tải lên: 14/02/2014, 22:20

... much slower than the rate of deacylation. The theoretical maxi- mum of the amplitude of the burst phase is equal to the total enzyme concentration. When k h1 ¼ 5 k 2 , only 2% of the maximum of the amplitude ... proton that gives rise to the normal isotope effect may be the proton that is transferred from the seryl oxygen to the seryl amino group during...

Ngày tải lên: 08/03/2014, 08:20

... operating at various steps in the cytotoxic activity of the drug, from a decrease of drug accumulation in the cell to abrogation of apoptosis induced by the chemical substance. Often several different ... sensitivity of tumor cells to chemo- therapy and is linked to activation of the phosphatidylinositol-3-kinase/Akt (PI3K/Akt) pathway. In the present study, we f...

Ngày tải lên: 07/03/2014, 03:20

... were calculated from the peak absorptions of the bands at pH 7.0 to provide a measure of coloration. b Data from [19]. c Data from [41]. AB DC Fig. 1. Comparison of spectral properties of the green -to- red ... imidazolyl group of the chromophore as in other green -to- red photoconverting proteins, due to a noncoplanar arrangement of the imidazole moiety with...

Ngày tải lên: 16/03/2014, 11:20

... the relevant organism [11–15]. Thus, the flavin cofac- tors mediate the successive transfer of two electrons from a two -electron donor, NADPH, to the obligatory one -electron acceptor moiety (the ... in the experimental buffers used in this work might be suffi- cient to account for the discrepancies. Because elec- trons have to pass from the FAD to the FMN co...

Ngày tải lên: 07/03/2014, 06:20

... inhibitors of human neutrophil elastase and proteinase 3 but not of cathepsin G. Both inhibitors belong, together with secretory leukocyte protease inhibitor, to the chelonianin family of canonical ... protease(s). To further characterize the maturation of elafin from trappin-2 and to compare the antiproteolytic activity of both inhibitors, we have expressed them in th...

Ngày tải lên: 07/03/2014, 15:20

... new mathematical model [22] to analyse the kinetics of conversion of violaxanthin to zeaxanthin. The model allowed us to follow independently the kinetics of the two de-epoxidation steps: the conver- sion ... antheraxan- thin, formed in such domains, has an immediate access to the VDE enzyme. Therefore, and in contrast to the de-epoxidation of violaxanthin...

Ngày tải lên: 08/03/2014, 10:20

... aggregates) to the sample. If the added lipid was a substrate for SCP-2, it would result in a decrease in the rate of transfer of BODIPY-PC because of the competition from the presence of a new ... resistant to the SCP-2-medi- ated lipid transfer as possible, and therefore the transfer protein preferentially transfers the labelled BODIPY-PC. To examine t...

Ngày tải lên: 16/03/2014, 12:20