Báo cáo khoa học: The noncatalytic C-terminus of AtPOLK Y-family DNA polymerase affects synthesis fidelity, mismatch extension and translesion replication ppt

... analysis of AtPOLK and AtPOLK n C We next analysed the catalytic efficiency and fidelity of AtPOLK and AtPOLKDC during DNA synthesis. To determine the fidelity of both full-length and truncated AtPOLK, ... signal, and a candidate PCNA-interaction domain. To better understand the role of the C-terminal domain of this Y-family DNA polymerase during DNA...

Ngày tải lên: 16/03/2014, 10:20

... showed that the upper band consisted of the a, b and c subunits in a molar ratio of 1.0 : 1.0 : 1.2. The lower band was composed of the a and c subunits in a molar ratio of 1.0 : 0.9. When the purified ... PAGE. Posi- tions of the top and middle bands coincided well with the two bands observed with the purified enzyme. Thus, it was suggested that the middle an...

Ngày tải lên: 21/02/2014, 03:20

... feature of the mechanism is the devel- opment of a strong hydrogen bond between the incoming nucleophile and the leaving group in the transition state of the reaction. The phosphate site of Schizophyllum ... phospho group of UDP- glucose and the side chains of Arg196 and Lys202 of family GT-4 mannosyltransferase PimA was only 2.44 and 2.77 A ˚ , respectiv...

Ngày tải lên: 30/03/2014, 04:20

... contributions MG, GC and EW participated in the collection and interpreta- tion of the data and were involved in drafting the manuscript. MG participated in analysis and interpretation of the data and in drafting ... and in drafting the manuscript. PS, JS and MV contributed to the conception and design of the study and manuscript revision. JK was involved...

Ngày tải lên: 25/10/2012, 10:39

... turns of the DNA double helix yields binding of the two or three NF-Y complexes on the same side of the DNA. Conservation of spacing is required for optimal pro- moter activity because changing the ... located upstream of the CDE-like site [46]. These observations and the fact that activation does not rely on CCAAT-boxes, NF-Y or Sp1 proteins, leave open the qu...

Ngày tải lên: 16/02/2014, 09:20

... helix 8 of the CTD which pack against the N-terminal end of helix 4 of the NTD of a neighboring subunit in the hexamer. Additional contacts involve helix 11 of the CTD and the C-terminal end of helix ... mutations Trp184Ala and Met185Ala). The X-ray model of the NTD hexamer from MLV, the superimposed model of the NTD hexamer from HIV-1, the high-resol...

Ngày tải lên: 18/02/2014, 06:20

... those of the assembly-competent mutants E187A and N183A: the conformations of the two assembly-incompetent mutants (Y169A and L211A) in the absence of CAI are the same as the structures of any ... disrupting the assembly of the virion capsid involves targeting the whole CA protein and regions close to the CTD. Thus, these peptides and small organic molecu...

Ngày tải lên: 18/02/2014, 06:20

... biological function of the pro-form of bone morphogenetic protein-2 (BMP-2), a member of the transforming growth factor b (TGFb) ⁄ BLP superfamily. The role of the 263 amino acids of the pro-peptide ... Kinetic constants for interaction of the ECD of BMPR-IA with the growth factors. Association (k a ) and dissociation (k d ) rates for the ligands with the ECD...

Ngày tải lên: 18/02/2014, 06:20

... Indeed, the enzyme stands at a branch point of the tryptophan to NAD pathway, and deter- mines the final fate of the amino acid, i.e. transformation into PA, com- plete oxidation through the citric ... molecule and a total of 216 solvent molecules. No electron density is present for the last four residues at the C-terminus. The stereochemistry of the model has be...

Ngày tải lên: 18/02/2014, 06:20

... example of the role of family 33 CBPs in the deg- radation of recalcitrant polysaccharides. Furthermore, the results provide the first example of a family 33 CBP that promotes the degradation of a-chitin, ... after 2 min of incubation with 1 n M of enzyme. The double peaks represent the a- and b-anomers of the oligomers. Using standard curves, the total conc...

Ngày tải lên: 18/02/2014, 08:20