Báo cáo khoa học: The noncatalytic C-terminus of AtPOLK Y-family DNA polymerase affects synthesis fidelity, mismatch extension and translesion replication ppt
Báo cáo khoa học: The noncatalytic C-terminus of AtPOLK Y-family DNA polymerase affects synthesis fidelity, mismatch extension and translesion replication ppt
... analysis of AtPOLK and AtPOLK nCWe next analysed the catalytic efficiency and fidelity of AtPOLK and AtPOLKDC during DNA synthesis. Todetermine the fidelity of both full-length and truncated AtPOLK, ... signal, and a candidate PCNA-interaction domain.To better understand the role of the C-terminaldomain of this Y-family DNA polymerase during DNA synthesis, we compared the catalytic properties of full-length ... of full-length AtPOLK and the truncated version(AtPOLKDC1–478). We found that deletion of the C-terminus increased the catalytic efficiency and fidelity of AtPOLK during synthesis on undamaged DNA ABFig....
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Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx
... showedthat the upper band consisted of the a, b and c subunits in amolar ratio of 1.0 : 1.0 : 1.2. The lower band was composed of the a and c subunits in a molar ratio of 1.0 : 0.9. When the purified ... PAGE. Posi-tions of the top and middle bands coincided well with the two bands observed with the purified enzyme. Thus, it wassuggested that the middle and top minor bands of the Ôcomponent BÕ ... addition of the othercomponent. Therefore, components A and B were assayedby adding an excessive amount of one component and making the other rate-limiting.PAGE and activity staining of glycerol...
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Báo cáo khoa học: The phosphate site of trehalose phosphorylase from Schizophyllum communeprobed by site-directed mutagenesis and chemical rescue studies pptx
... feature of the mechanism is the devel-opment of a strong hydrogen bond between the incoming nucleophile and the leavinggroup in the transition state of the reaction. The phosphate site of Schizophyllum ... phospho group of UDP-glucose and the side chains of Arg196 and Lys202 of family GT-4 mannosyltransferase PimA was only 2.44 and 2.77 A˚, respectively [23]. Removal of either one of the two strong ... phos-phate and oligosaccharide show that hydrogen bondsfrom the NE and NH2 atoms of the guanidine sidechain of Arg535 to two oxygen atoms of phosphatedominate the contacts between the enzyme and the phosphate...
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Báo cáo khoa học: "The clinical value of daily routine chest radiographs in a mixed medical–surgical intensive care unit is low"
... contributionsMG, GC and EW participated in the collection and interpreta-tion of the data and were involved in drafting the manuscript.MG participated in analysis and interpretation of the data and in drafting ... and in drafting the manuscript. PS, JS and MV contributed to the conception and design of the study and manuscript revision.JK was involved in the design and statistical analysis of the study. MS ... University of Amsterdam, Amsterdam, The Netherlands2Resident, Departments of Intensive Care Medicine and Internal Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands3Resident,...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
... turns of the DNA double helix yields binding of the two orthree NF-Y complexes on the same side of the DNA. Conservation of spacing is required for optimal pro-moter activity because changing the ... located upstream of the CDE-like site [46].These observations and the fact that activation doesnot rely on CCAAT-boxes, NF-Y or Sp1 proteins,leave open the question of whether the BUB1B pro-moter ... footprinting.Loss of the two pocket proteins leads to deregulation of the B-Myb promoter during the cell cycle. Re-intro-duction of p107, and to some extent also p130, causesrepression of the B-Myb...
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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeficiency virus: intersubunit interactions during virus assembly doc
... helix 8 of the CTDwhich pack against the N-terminal end of helix 4 of the NTD of a neighboring subunit in the hexamer.Additional contacts involve helix 11 of the CTD and the C-terminal end of helix ... mutationsTrp184Ala and Met185Ala). The X-ray model of the NTD hexamer from MLV, the superimposed model of the NTD hexamer fromHIV-1, the high-resolution X-ray model of the modifiedCA hexamer from HIV-1, and the ... [32], and of the mature capsids of differentretroviruses; (c) the different structural organization of CA in the immature and mature capsids, including the arrangement and spacing of the hexameric...
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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeficiency virus: designing inhibitors of capsid assembly ppt
... those of the assembly-competent mutants E187A and N183A: the conformations of the two assembly-incompetentmutants (Y169A and L211A) in the absence of CAIare the same as the structures of any ... disrupting the assembly of the virion capsidinvolves targeting the whole CA protein and regionsclose to the CTD. Thus, these peptides and smallorganic molecules, together with the structures of the complexes ... the X-ray structure of the nonmu-tated-CTD–CAI complex [28] (Fig. 2A, main panel). The aromatic moiety of Phe3, together with Leu6 and Ile1, are the residues of CAI with the largest numberof...
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Tài liệu Báo cáo khoa học: The pro-form of BMP-2 interferes with BMP-2 signalling by competing with BMP-2 for IA receptor binding pptx
... biological function of the pro-form of bone morphogenetic protein-2 (BMP-2), a member of the transforminggrowth factor b (TGFb) ⁄ BLP superfamily. The role of the 263 amino acids of the pro-peptide ... Kinetic constants for interaction of the ECD of BMPR-IAwith the growth factors. Association (ka) and dissociation (kd) ratesfor the ligands with the ECD and the apparent dissociation con-stants ... interested in the role of the 263amino acid pro-peptide of BMP-2, because evidenceaccumulated over recent years has shown that the pro-forms of growth factors can modulate the activities of the mature...
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Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt
... Indeed, the enzymestands at a branch point of the tryptophan to NAD pathway, and deter-mines the final fate of the amino acid, i.e. transformation into PA, com-plete oxidation through the citric ... molecule and a total of 216 solvent molecules. Noelectron density is present for the last four residues at the C-terminus. The stereochemistry of the model hasbeen assessed using the program ... Arg47 and the Phe294-Pro295-Leu296 amino acid stretch (Fig. 3A,B). The ligand binds in an extended conformation, with itsphosphate moiety located in the proximity of the zincion and with the...
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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... example of the role of family 33 CBPs in the deg-radation of recalcitrant polysaccharides. Furthermore, the results provide the first example of a family 33CBP that promotes the degradation of a-chitin, ... after 2 min of incubation with 1 nM of enzyme. The double peaks represent the a- and b-anomers of the oligomers. Using standardcurves, the total concentrations of dimer, trimer and tetramer ... shown in the figure. (C) Time course of the degradation of (GlcNAc)3(r) and (GlcNAc)4( )byLlChi18A, illustratedby the production of (GlcNAc)2during the initial linear phase of the degradation...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ