Báo cáo khoa học: Enzymes that hydrolyze adenine nucleotides of patients with hypercholesterolemia and inflammatory processes potx
Báo cáo khoa học: Enzymes that hydrolyze adenine nucleotides of patients with hypercholesterolemia and inflammatory processes potx
... Enzymes that hydrolyze adenine nucleotides of patients with hypercholesterolemia and inflammatory processes Marta Medeiros Frescura Duarte 1 ,Va ˆ nia ... understand their active role in this process and the importance of such hydrolysis. In line with this, literature data indi- cate that adenine nucleotides and adenosine are important modulators of ......
Ngày tải lên: 16/03/2014, 10:20
Báo cáo khoa học: Reconstruction ofde novopathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose inSaccharomyces cerevisiae pptx
... detected as a 40-kDa band within the TOY2 and TOY3 strains (Fig. 2A). In vitro activities of UGD and UXS We next examined the activity of the hybrid enzymes from cytoplasmic fractions of the recombinant ... we expressed the AtUGD1 and AtUXS3 genes, which encode UGD and UXS of A. thaliana, with the hope of producing UDP-GlcA and UDP-Xyl in yeast. We found that introdu...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras potx
... TnC(1–91) and TnI(98–182) form a regula- tory subunit, and that the first half of the C termi- nus of TnI is important for this binding. Structural information of the interaction of TnC and TnI ... results are similar with the results observed for the binary complexes formed by TnC(1–91) and TnI(1–147), and TnC and TnI [16] and agree with the hypothesis that the e...
Ngày tải lên: 16/03/2014, 18:20
Tài liệu Báo cáo khoa học: Anaerobic sulfatase-maturating enzyme – A mechanistic link with glycyl radical-activating enzymes? docx
... PAGE 12.5%) of WT and the M 1 ,M 2 and M 3 variants of anSMEbt. Fig. S4. UV-visible absorption spectra of oxidized and dithionite-reduced as purified and reconstituted forms of WT and M1 mutant ... (A) and MALDI-TOF MS (B) analysis of the peptide maturation catalyzed by WT anSMEbt and by M 1 ,M 2 and M 3 mutants of anSMEbt. The WT and mutant forms of anSMEbt (ea...
Ngày tải lên: 16/02/2014, 14:20
Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
... of ISP into an immature bc 1 complex and that the association of the Qcr9p subunit with the bc 1 complex precedes the binding of ISP. In fact, the direct absence of ISP (DISP), or the block of its ... new findings open up several avenues of investigation and illustrate that a significant amount of work is still neces- sary for a complete understanding of the assembly p...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc
... oxidative activity with glycerol and NADP. Also, with other C4 and C5 polyols no activity with NADP as a cofactor was observed. We tested erythritol, ribitol, xylitol and dl-arabinitol at a concentration of ... electro- phoresis, and enzyme activities with NADP and gly- cerol as substrates were visualized by Zymogram staining; that is, only enzymes that had activity...
Ngày tải lên: 19/02/2014, 06:20
Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx
... up-regulation of the expression and enzymatic activity of both AGAT and GAMT. The specific activity of AGAT in sarcoma tissue reached almost 50% of that observed in the kidney and it was also observed that ... formation of polyamines and methionine. An investigation of the level of different intermediate metabolites and the expression of different enzymes in the...
Ngày tải lên: 07/03/2014, 04:20
Báo cáo khoa học: Enzymes of mannitol metabolism in the human pathogenic fungusAspergillus fumigatus– kinetic properties of mannitol-1-phosphate 5-dehydrogenase and mannitol 2-dehydrogenase, and their physiological implications pot
... binding of Man-ol1P and NAD + by AfM1PDH is not ordered, and that there is also randomness in the binding of substrate and coen- zyme in each direction of the reaction catalyzed by AfM2DH. By way of ... also reported random binding of NAD + and Man-ol1P [29]. It is reasonable to assume that random binding of reactants by AfM1PDH and AfM2DH occurs in rapid equilibrium...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Enzymes in organic media Forms, functions and applications potx
... enzymatic reactions; synthesis and modification of polymers. Introduction The use of enzymes in organic media (with low water content) has been one of the most exciting facets of enzymology in recent ... precipitated with and rinsed with propanol [29], enzymes obtained after three-phase partitioning [30], cross- linked enzyme crystals [22], crosslinked enzyme aggregates [3...
Ngày tải lên: 16/03/2014, 16:20
Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
... was recovered and mixed with an equal volume of SDS buffer (0.2 m Tris, pH 7.5, 0.3 m NaCl, 25 mm EDTA, 2% SDS) and twice extracted with phenol ⁄ chloroform, once extracted with chloroform, and precipitated ... the fate of transcripts with which it becomes associated. Materials and methods Materials Enzymes were purchased from Invitrogen (Carlsbad, CA, USA) and Roche (...
Ngày tải lên: 16/02/2014, 15:20