Báo cáo khoa học: Intracellular degradation of somatostatin-14 following somatostatin-receptor 3-mediated endocytosis in rat insulinoma cells doc

... compilation ª 2008 FEBS 4739 Intracellular degradation of somatostatin-14 following somatostatin-receptor 3-mediated endocytosis in rat insulinoma cells Dirk Roosterman 1 , Nicole E. I. Brune 2 , ... Agonist-mediated endocytosis of rat somato- statin receptor subtype 3 involves beta-arrestin and clath- rin coated vesicles. J Neuroendocrinol 13, 279–287. Intracel...

Ngày tải lên: 16/03/2014, 06:20

... cystatin C is the most discriminating residue for binding to cathepsins B, H, L, S [24]. No L9K mutants of cystatin C have been prepared yet to study the effect of incorporating the Cystatin F in ... activation of cystatin F we have studied the in vitro kinetics of the interaction between cystatin F and several cathepsins, as well as their intracellular localization in promono...

Ngày tải lên: 19/02/2014, 17:20

... Regardless of dopamine treatment, overexpres- sion of a-synuclein significantly affected the levels of four proteins [stathmin 1, glutathione S-transferase (GST)p, Ran 1 binding protein and C1q binding ... suggested to be linked to early stages of PD pathogenesis [26]. Interestingly, in our model, dopamine induces an increase of the actin bundles regulator fascin 1 [27] and discord...

Ngày tải lên: 15/02/2014, 01:20

... also observed, includ- ing that Mxi1-SRa appears to be the predominant transcript in the adult intestine and in the developing embryo, whereas Mxi1-SRb transcripts predominate in the adult liver ... Schematic representation of the different Mxi1 isoforms tested for suppres- sive potential in the REF transformation assay. All of the isoforms carried Myc tags on their COOH termini....

Ngày tải lên: 18/02/2014, 16:20

... (HRP-labelled) (1 : 500)]. Haematoxylin was employed for counterstaining. Haematoxylin–eosin staining Haematoxylin–eosin staining was carried out following the routine histological method described ... absorptive site of folic acid, i.e. the small intestine. Expression of the RFC protein in BBM of the intestine The effect of chronic alcoholism on the level of expres- sion of t...

Ngày tải lên: 18/02/2014, 16:20

... determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing the three distinctive domains of tandem repeat ... P i , and specific binding of the antibody was detected by incubating the sections in a solution of 10 mm diaminobenzidine in 10 mm NaCl ⁄ P i . H. Tohse et al. A novel protein in...

Ngày tải lên: 18/02/2014, 17:20

... determination of the BcZBP protein at a resolution of 1.8 A ˚ , provides important clues towards understanding the enzyme function, including the identification of a zinc-binding site within a ... proteins. B. cereus thus provides a convenient model organism for studying proteins potentially associated with the pathogenicity of the highly infectious B. anthracis. The zinc-binding prote...

Ngày tải lên: 19/02/2014, 00:20

... effects detected at 96 h in cultures transfected with vectors containing or lacking an insert indicate that after 48 h, other interacting factors could modify the molecular phenotypic effect of using this vector. ... because of the import- ance of this enzyme in the parasite’s intraerythrocyte stage [16]. The present paper describes the effects of G6PD-6PGL silencing in P. falci...

Ngày tải lên: 19/02/2014, 07:20

... fluorescence in HepG2 cells grown without (thin line, – Fe) or with (thick line, + Fe) iron in one representative of five separate experi- ments. Dotted line represents background staining in HepG2 cells ... (thick lines, + saponin) cells cul- tured without and with iron as indicated. (D) Perls’ staining of cyto- spins of HepG2 cells grown in media supplemented with 100 l...

Ngày tải lên: 19/02/2014, 16:20

... on plasmin(ogen) binding to fibrin and CHO-K1 cells, fibrin plates and cells were incubated with a fixed concentration of plasmin(ogen) supplemented with varying concentrations of mAbs. Bound plasmin(ogen) ... maximal rate of plasmin formation in the absence of 6-Ahx. Data were fitted as indicated in Fig. 1 (IC 50 ¼ 5mM). (B) Kinetics of plasmin formation performed on cells (ma...

Ngày tải lên: 20/02/2014, 01:20