Báo cáo khoa học: Importance of tyrosine residues of Bacillus stearothermophilus serine hydroxymethyltransferase in cofactor binding and L-allo-Thr cleavage Crystal structure and biochemical studies pot

... of tyrosine residues of Bacillus stearothermophilus serine hydroxymethyltransferase in cofactor binding and L-allo-Thr cleavage Crystal structure and biochemical studies B. S. Bhavani 1, *, V. ... The superposition of Y61A bsSHMT–Gly and bsSHMT– Gly–FTHF shows that the mutation of Y61 to A cre- ates a large cavity near the binding site of the pt...

Ngày tải lên: 16/03/2014, 06:20

... distance between the two termini of the inserted Ca 2+ -binding sites in the model structure of the EF-hand of STIM1 is within 15 A ˚ . Accordingly, a total of six glycine linkers is suffi- cient to ... dimerization and calcium binding in an EF-hand synthetic peptide. J Theor Biol 199, 199– 211. 12 Julenius K, Robblee J, Thulin E, Finn BE, Fairman R & Linse S (2002) Cou...

Ngày tải lên: 07/03/2014, 00:20

... of residues: 26–121 and 325–343 (chain A), 266–295 (chain B) and 385–399 (chain C) in SmPAL out ⁄ in and 33–128 and 333–351 (chain A), 272–303 (chain B) and 293–306 (chain C) in PlPAL out ⁄ in . ... taxol biosynthesis in Taxus chinensis [27] or T. cuspidata [28] and tyrosine 2,3-aminomutase (TAM), which is involved in biosynthesis of a natural product having pot...

Ngày tải lên: 07/03/2014, 12:20

... 2009). For example, even in case of coordination, coordinating con- junction is the head and conjuncts are children of the coordinating conjunction. The coordinating conjunction is attached ... 4.1 Hindi Recently in NLP Tools Contest in ICON-2009 (Husain, 2009 and references herein), rule-based, constraint based, statistical and hybrid approach- es were explored for par...

Ngày tải lên: 07/03/2014, 22:20

... SelectingAntecedent ( INPUT L: ListOfCandidates, OUTPUT Solution: Antecedent ) Apply restrictions to L with a result of L1 Morphologic agreement C-command constraints Semantic consistency Case of: NumberOfElements ... the same number of parsed constituents as the anaphora 6) Candidates that have appeared with the verb of the anaphor more than once 7) Preference for indefinite NPs. Ca...

Ngày tải lên: 08/03/2014, 05:20

... identified N302 and I371 as key determinants of substrate binding and orientation for catalysis. K484 is not involved in initial substrate binding, but seems to play a significant role in catalysis, ... results in close to 50% loss in C4H activity, does not affect I2C hydroxylation. As initial cinnamate binding is not in u- enced by this mutation (Table 2) and binding ki...

Ngày tải lên: 17/03/2014, 10:20

... position of Lys78 is anchored with an interaction between the main chain oxygen of Ala216 and the e-amine of Lys78. In H34A mutant (Fig. 6B), the interaction between the imidazole ring and Asp214 ... reported secondary structures of pepsin-like aspartic proteases which are predominately b sheet [16]. On the basis of the kinetic behavior of the H34A and S37A mutants and t...

Ngày tải lên: 23/03/2014, 07:20

... roles in substrate binding and the specificity and catalysis of enzymes [27–29], as well as in stabiliz- ing tertiary and quaternary structure [30–32]. On the basis of previous crystal- structure ... For the ligand complexes, crystals were immersed for < 1 min in a drop of stabilizing solution containing a grain of solid ligand before being vitrified. All data sets...

Ngày tải lên: 29/03/2014, 09:20

... positioning of inhibitors in alternate configurations [34]. The importance of gorge dimensions in accommodating bulky inhibitors was also seen in the binding of propidium, decamethonium, tacrine and ... structure regarding the role of Phe330 in the binding and selectivity of inhibitors to AChE, is not unique to E2020 and was noted for huperzine A and tacrine a...

Ngày tải lên: 30/03/2014, 20:20

... Asp188, Ala208 and Gly234). Some of the res- idues involved in phosphate and calcium binding are conserved in the PhyH-DII sequence, whereas only one phosphate -binding residue is conserved in the PhyH-DI ... presence of Ca 2+ increased the thermal stability of PhyH and PhyH-DII. After incubation at 60 °C for 30 min, both enzymes retained > 70% of their initial activ...

Ngày tải lên: 14/02/2014, 15:20