Báo cáo khoa học: Internalization of cystatin C in human cell lines docx

... measurement of the cysteine inhibitory capacity of cells. The human cancer cell lines chosen included one epidermoid carci- noma cell line, three mammary tumour cell lines and one human pancreas adenocarcinoma. ... protein )1 (Fig. 4), as expected for a protein secreted as a result of the cellular production of cystatin C. Cellular levels of cystatin C after in...

Ngày tải lên: 16/03/2014, 06:20

... Proceedings of EACL '99 Determination of Syntactic Functions in Estonian Constraint Grammar Kaili Mfifirisep Institute of Computer Science University of Tartu Liivi 2, ... last stage of the analysis indicate its syntactic functions. The underlying principle in determin- ing both the morphological interpretation and the syntactic functions is the same: first all ......

Ngày tải lên: 24/03/2014, 03:21

... sequence variants is unclear, but increas- ing evidence indicates that minor sequence variations in linker histones may change their binding characteristics, in uence chromatin remodeling, and specifically ... utility of HILIC as a means of investigating the occurrence of sequence variations within linker histone subtypes from various human tumor cell lines. By using HILIC we det...

Ngày tải lên: 30/03/2014, 20:20

... the dinuclear copper center of tyrosinase is flexible during catalysis. J Biol Chem 281, 8981–8990. 8 Klabunde T, Eicken C, Sacchettini JC & Krebs B (1998) Crystal structure of a plant catechol ... present in the sequence are the two copper A (amino acids 86–96) and copper B (amino acids 258–294) binding motifs common to most tyrosinase sequences [3] that contain five of the six co...

Ngày tải lên: 06/03/2014, 11:20

... G-3¢; HCV-1b Core+1/S sense, 5¢-ATC CGG GGT CTC CCATG GCA ATG AGG GCC TGG GGT G-3¢; HCV-1a Core+1/S antisense, 5¢-AT CCG GGT CTC GGTACC TTA TCA CGC CGT C TT CCA GAA C- 3¢; and HCV-1b Core+1/S antisense, ... using a template plasmid pRSV/BNT (kindly provided by C. Bre ´ chot) [69] and the following primers: sense, 5¢-CAT G CC ATG GCA CCA ACC GCC GCC CAC A-3¢; and antisense, 5¢-CCC AAG CTT...

Ngày tải lên: 15/03/2014, 09:20

... A. (1991) The effect of elimination of intersubunit disulfide bonds on the activity, assembly, and secretion of recombinant human acetylcholinesterase. Expression of acetylcholinesterase Cys-580fiAla ... associated with the N-terminal Q N fragment of ColQ, containing the proline-rich attachment domain motif) com- plexes were secreted, but undetectable or barely detectable in cell...

Ngày tải lên: 23/03/2014, 12:20

... beck.com Abbreviations: b-arr1, b-arrestin 1; CRF, corticotropin releasing factor; CRFR1, corticotropin releasing factor receptor 1; EGFP, enhanced green fluorescence protein; GPCR, G-protein coupled receptor; ... details of CRFR1 r egulation such a s internalization remain unchar- acterized. In the present study, agonist-induced internal- ization of CRFR1 in HEK293 cells was v isual...

Ngày tải lên: 30/03/2014, 15:20

... phos- phorylation of CYP1A1 ⁄ 1A2 occurred at 24 h after Sham GdCl 3 CLP GdCl 3 + CLP CYP1A1 β-actin β-actin β-actin β-actin CYP2B1 CYP2E1 CYP1A2 Fig. 4. Effects of KCs on hepatic CYP1A1, 1A2, 2B1 ... red pulp mac- rophages transiently disappear. The white pulp Sham GdCl 3 CLP GdCl 3 + CLP CYP1A1 β-actin β-actin β-actin β-actin CYP2B1 CYP2E1 CYP1A2 Fig. 2. Effects of KCs on hepatic C...

Ngày tải lên: 14/02/2014, 18:20

... of a variety of cell types, including hepatocytes, to long-chain saturated fatty acids (SFAs) led to increased expression of proin- flammatory cytokines, inhibition of insulin signaling, induction ... periods of 3, 6, 12, 24 and 36 h, before staining of the accumulated neutral lipids with Nile red and analysis of the fluorescence intensity of individual cells by flow cytometry....

Ngày tải lên: 14/02/2014, 22:20

... nuclear extracts from SP1 cells. The nucleo- tide sequences of putative CERK-PPRE, including the sense 5¢-CTCTCCAGGCCACAGGCCAGAGCGG-3¢ and anti-sense 5¢-GAGAGGTCCGGTGTCCGGTCTCGCC-3¢ sequences, ... 5¢-CTGC CCAAGGGTGCTGTGCTC-3¢ and for reverse 5¢-TTGTT CTCACCAGACCCTTGAC-3¢. All reactions were run with a hot-start preincubation step of 10 min at 95 C, followed by cycles of 15 s at 95 C an...

Ngày tải lên: 18/02/2014, 18:20