Báo cáo khoa học: The Janus-faced atracotoxins are specific blockers of invertebrate KCa channels ppt

... as these other toxins. This is probably due to the bifurcated d-guanidinium group at the tip of the critical Arg8 residue, which is much bulkier than the single amine moiety at the tip of the ... Journal 275 (2008) 4045–4059 ª 2008 The Authors Journal compilation ª 2008 FEBS 4059 The Janus-faced atracotoxins are specific blockers of invertebrate K Ca channels...

Ngày tải lên: 16/03/2014, 06:20

... However, neither the specificity of bacitracin for PDI nor the detailed mech- anisms of inhibition of PDI have been investigated. Furthermore, since the original reporting of the inhibi- tion of PDI ... mechanism of action, the reaction conditions of the assay were var- ied. The removal of EDTA from the reaction signifi- cantly reduced the noncatalyzed rate (Fi...

Ngày tải lên: 16/02/2014, 14:20

... and PLC are capable of rapidly elevating the level of DAG. In agreement with these reports, we found that the addition of 100 l M ATP doubled DAG mass within 5 min; interest- ingly, the presence of ... volume of 55 lL. For the specific measurement of the atypical PKC isoenzyme-f,Ca 2+ was omitted from the assay, 80 lgÆmL )1 phosphatidylserine was substituted for th...

Ngày tải lên: 20/02/2014, 02:21

... proteins; M pep , the number of unique matched peptides; U pep , the number of unmatched peptides in the MALDI-TOF experiments. The function of new proteins is based on the GH family of the catalytic ... half the activity of F5. All in all, these results indicate that the differences in the protein composition of the cellulosomes are related to different enzy...

Ngày tải lên: 18/02/2014, 08:20

... encoded another type of KLK11, isoform 2, despite the presence of exon 1c. The secretory path- ways of KLK11 isoforms appeared to differ, although both isoforms were secreted from cells. Hence, the ... raised against KLK11 was the same size as that of the translational product by isoform 2 mRNA (Fig. 4B). The product by isoform 1 mRNA was smaller than the isoform 2 produ...

Ngày tải lên: 19/02/2014, 06:20

... Many diseases are associated with changes to sHSPs occurring either as a result of mutation or by post-translational changes, and these are outlined below and described in the text of the review. ... actin filament modifications. Synthesis of exogenous human aA-crystallin in lens epithelial cells of the same species counters UVB-induced apoptosis by favoring action of the...

Ngày tải lên: 19/02/2014, 18:20

... and refer- ence samples. The presence of the internal standard probes at different locations of the array allowed quantification of the local background and evaluation of the array homoge- neity, ... into account in the normalization. Furthermore, in order to consider the purity and quality of the mRNA, a second normalization step was performed on the basis of th...

Ngày tải lên: 07/03/2014, 05:20

... with the substrate specificity observed for the recombinant E1 enzyme in the absence of the other complex components [14]. The relative activities are given in Table 1, along with those for the ... using the program SEDFIT, and the data were then directly fitted using the finite element solution of the Lamm equation in SEDFIT [18] to give values of the sedimentation...

Ngày tải lên: 16/03/2014, 05:20

... superposition of the backbone atoms of ligand binding residues. These are defined as the residues within 4 A ˚ of any atom of either paclitaxel in the 1JFF structure or epo- thilone in 1TVK. They are: ... compar- ison of the complete structures and of the most rele- vant parts of the structures (dimer interface and binding site). The differences in the overall...

Ngày tải lên: 16/03/2014, 13:20

... raises the question of whether this represents an exception or rather a particular example of a general strategy of viral subversion of chemokine receptor functions. It should be noted that proof of ... Research, Division of Medicinal Chemistry, the Netherlands 3 Department of Medical Microbiology, University Hospital of Maastricht, the Netherlands 4 Institute of Bioch...

Ngày tải lên: 16/03/2014, 22:20