Báo cáo khoa học: Factors influencing RNA degradation by Thermus thermophilus polynucleotide phosphorylase ppt
Báo cáo khoa học: Factors influencing RNA degradation by Thermus thermophilus polynucleotide phosphorylase ppt
... et al. 2226 FEBS Journal 275 (2008) 2214–2226 ª 2008 The Authors Journal compilation ª 2008 FEBS Factors influencing RNA degradation by Thermus thermophilus polynucleotide phosphorylase Marina ... 3¢ end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core. Mol Cell 20, 473–481. 46 Singer MF (1958) Phosphorolysis of oligoribonucleo- tides by poly...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Highly site-selective stability increases by glycosylation of dihydrofolate reductase ppt
... to glycosyl- ation, all proteins were > 95% pure as judged by SDS–PAGE. Glycosylation was confirmed by tryptic digestion followed by MALDI-TOF MS (Fig. S1). Ligand binding and kinetics of glycosylated ... being simply caused by blanket coverage of large regions of the protein surface. Alternatively, increases in stability L H. Tey et al. Glycosylation of E. coli DHFR FEBS Journal 27...
Ngày tải lên: 15/03/2014, 11:20
Báo cáo khoa học: High thermal and chemical stability of Thermus thermophilus seven-iron ferredoxin Linear clusters form at high pH on polypeptide unfolding doc
... ferredoxin; linear iron–sulfur cluster; protein unfolding; thermostability; Thermus thermophilus. Many proteins require the binding of cofactors to perform their biological activity. It has been demonstrated ... interactions with their cofactors after polypeptide unfolding [1–6]. Therefore, it is possible that cofactors bind to their corresponding polypeptides before or during folding in...
Ngày tải lên: 16/03/2014, 23:20
Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... comments and suggestions. This work was supported, in part, by an International Grant from the Howard Hughes Medical Institute to J.L.S. and by grants from Programa de Nu´ cleos de Exceleˆ ncia (PRONEX), ... analysis of the sequence-specific binding of RNA by bacteriophage MS2 coat protein. Proc. Natl Acad. Sci. USA 95, 9244–9249. 11. Ling,C.M.,Hung,P.P.&Overby,L.R.(1969)Specificity...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase pot
... was performed using a simplified method, described previously by Xiang & Edmondson [22]. N-terminal sequence analysis was obtained by automated Edman degradation of purified XDH blotted onto poly(vinylidene ... standards. Cloning and sequencing of the C. acidovorans xdh genes by PCR Eleven polypeptides, sequences of which were obtained by Edman degradation of C. acidovorans XDH...
Ngày tải lên: 16/03/2014, 23:20
Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx
... aggregation P. N. Jethva et al. 1698 FEBS Journal 278 (2011) 1688–1698 ª 2011 The Authors Journal compilation ª 2011 FEBS Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and ... of age. The etiological factors that are involved in the development of Parkin- son’s disease include genetic factors, susceptibility to various drugs and environmental factors [3–5]....
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
... lipase C. Krintel et al. 4762 FEBS Journal 276 (2009) 4752–4762 ª 2009 The Authors Journal compilation ª 2009 FEBS Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes ... Journal 276 (2009) 4752–4762 ª 2009 The Authors Journal compilation ª 2009 FEBS levels of cAMP, which causes the catalytic subunits of PKA to dissociate from the regulatory subunits and thereb...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Attenuation of cardiac mitochondrial dysfunction by melatonin in septic mice ppt
... assay of iNOS mRNA expression Quantification of the iNOS mRNA levels was done by SYBR green two-step real-time RT-PCR (Stratagene Mx 3005P; Stratagene, La Jolla, CA, USA). Total cellular RNA was isolated ... Effects of aMT treatment on CLP-induced mRNA levels of iNOS. Ten nanograms of RNA extracted from mouse heart was used, and quantification of iNOS mRNA was performed by real-time RT-...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: Changes in purine specificity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx
... bio- chemically one by one. Abbreviations AC, adenylate cyclase; PDE, phosphodiesterase; mPDE2, mouse PDE2; rPDE2, rat PDE2. 1514 FEBS Journal 274 (2007) 1514–1523 ª 2007 The Authors Journal compilation ... al. Cyclic nucleotide specificity in GAF domains FEBS Journal 274 (2007) 1514–1523 ª 2007 The Authors Journal compilation ª 2007 FEBS 1515 by neither cAMP nor cGMP up to 10 mm (Fig. 2A)...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... reduced by increasing the temperature, by adding the organic solvent trifluoroethanol (TFE) or by seeding [6]. The time course for morphological changes occurring during the amyloid fibril formation by ... et al. Copper binds to cystatin B FEBS Journal 273 (2006) 4250–4263 ª 2006 The Authors Journal compilation ª 2006 FEBS 4263 High affinity copper binding by stefin B (cystatin B) and i...
Ngày tải lên: 19/02/2014, 06:20