Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot

... 2008) doi :10 .11 11/ j .17 42-4658.2008.06372.x Mammalian target of rapamycin complex 1 (mTORC1) phosphorylates proteins such as eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and the S6 kinases. ... of initiation factor 4E-binding protein 1 in vivo. J Biol Chem 278, 40 717 –40722. 20 Wang X & Proud CG (2006) The mTOR pathway in the contr...

Ngày tải lên: 16/03/2014, 06:20

... revealed that these three proteins dif- fused with diffusion constants of 11 .7, 12 .9 and 5.4 lm 2 Æs )1 , respectively, in the part of the cytoplasm outside of the granules. FRAP analysis in the gran- ules ... from fluctuations of probes provides the diffusional proper- ties of proteins [13 ] and binding interactions [14 ]. FRAP is a conventional technique used...

Ngày tải lên: 18/02/2014, 16:20

... YXXXK), RDH2 1) 137 (oligonucleotides 10 14 ; amino acids 1 13 7, truncated after the GLVNNAG region), and RDH2 1) 58 (oligonucleotides 10 15 ; amino acids 1 58, truncated just after the cofactor-binding sequence ... activity [12 ]. The specific activity of each amphioxus enzyme was 0.25 nmol of retinolÆmin )1 Æ(mg of microsomes) )1 for RDH1 incubated with 15 lm all-tra...

Ngày tải lên: 07/03/2014, 09:20

... 0.5% Triton X -10 0, 10 lgÆmL )1 aprotinin). After determination of the protein concentration, cell lysate containing 10 0 lgofproteinwas diluted to 300 lL with lysis buffer, added 15 lLof immobilized ... the cDNA encoding human P-LAP [3]. Analysis of the cDNA indicates that the enzyme is a type II membrane-spanning protein, which belongs to the M1 family of zinc-met...

Ngày tải lên: 08/03/2014, 02:20

... the accessi- bility of the active site was better in the protein bear- ing the N2 domain than in the protein bearing the Nc domain. Thus, the accessibility of the active site in the N2 proteins ... the protein in the presence of the water–lipid interface. Also, both the N-terminal and C-terminal domains of hoPL were involved in the inactivat...

Ngày tải lên: 16/03/2014, 06:20

... 607 213 49 1. 1 36.9 10 87.3 759.9 B 16 2 16 8 2 910 14 854 18 .0 88.4 313 5.5 836.4 C 16 2 16 6 3435 16 166 21. 2 97.4 3228.9 837.7 estimated. In the second approach, on the other hand, as described in the ... con- structed from the result of cross-language retrieval of relevant news articles in section 3 .1. Referring to the definition of the set D of r...

Ngày tải lên: 22/02/2014, 02:20

... ade2 -1 his3D200 leu2-3, -11 2 trp1D1 ura3 -1 can1 -10 0 B. Dujon, (Institut Pasteur, Paris, France) AEMY25 MATa ade2 -1 his3 -11 , -15 leu2-3, -11 2 trp1D1 ura3 -1 lsm1D::TRP1 [1] MPS 11 MATa ade2 -1 his3 -11 , -15 ... leu2-3, -11 2 trp1D1 ura3 -1 can1 -10 0 lsm8D::TRP1 [pRS 313 , P GAL -HA-LSM8] LSM7 :13 myc-HphMX6 [13 ] MPS26 MATa ade2 -1 his3 -11 , -15 leu2-3, -11 2 trp1D1 ura3...

Ngày tải lên: 07/03/2014, 02:20

... combination of a potent quinolone combined with clindamycin. This strategy is commonly followed in our facility for infection of unknown origin and generally corresponds to the guidelines of the ... decompression [12 ]; (ii) increasing the vascularity of the wound, thus improving cutaneous perfusion [13 ,14 ]; (iii) promoting granulation tissue formation [15 ,16 ]; and/or (i...

Ngày tải lên: 25/10/2012, 10:41

... 4490 Tel: + 81 29 853 5600 (5466) E-mail: yokokawa@ims.tsukuba.ac.jp (Received 9 March 2 011 , revised 24 May 2 011 , accepted 16 June 2 011 ) doi :10 .11 11/ j .17 42-4658.2 011 .08222.x Studies of ion pumps, ... ion transport reaction [14 ,16 19 ]. The single line scanning method [8 ,15 ], in which an AFM probe repeatedly scanned on a single line (along the y-axis direction in F...

Ngày tải lên: 14/02/2014, 14:20

... 278 (2 011 ) 11 90 12 02 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 11 99 pathways. Injected CagA associates with the epithelial tight-junction scaffolding protein, zona occludens -1, and the ... November 2 010 , revised 11 January 2 011 , accepted 27 January 2 011 ) doi :10 .11 11/ j .17 42-4658.2 011 .08035.x Helicobacter pylori is a very successful human-specific bacte...

Ngày tải lên: 14/02/2014, 19:20