Báo cáo khoa học: Structural basis for recognition of Co2+ by RNA aptamers pot

... 878–892. Recognition of Co 2+ by RNA aptamers J. Wrzesinski and S. K. Jo ´ z ´ wiakowski 1662 FEBS Journal 275 (2008) 1651–1662 ª 2008 The Authors Journal compilation ª 2008 FEBS Structural basis for ... grateful to Professor Jerzy Ciesiolka for critically reading the manuscript and for helpful Recognition of Co 2+ by RNA aptamers J. Wrzesinski and S. K. Jo ´ z ´...

Ngày tải lên: 16/03/2014, 06:20

... 112–116. Study of the interactions of the Anp32a LRR module C. de Chiara et al. 2560 FEBS Journal 275 (2008) 2548–2560 ª 2008 The Authors Journal compilation ª 2008 FEBS Structural bases for recognition of ... a study of the structural determinants for the interactions of Anp32a with other partners. As a prerequisite for binding stud- ies, we first solved the structure in...

Ngày tải lên: 18/02/2014, 17:20

... positions and the conformations of the three loops of EcoMBP are different from those of TvuCMBP. Trp62, located at region N-I, is found at the center of the curved form of maltotetraose (Fig. ... that of TvuCMBP (Fig. 7B). Similar protrusions, which are formed by Asp209 and Arg344, are observed at the entrance of the cleft of EcoMBP, but the distance between Od2 of Asp209...

Ngày tải lên: 19/02/2014, 00:20

... a hypothesis for the basis of sub- strate recognition in this group of so-called ‘append- age-dependent xylanases’ [11]. Examination of the structure of XynA [14] for the presence of aromatic amino ... were performed with leadfinder [23]. This program was also used for the preparation of the protein structure for dock- ing by addition of hydrogen atoms according to...

Ngày tải lên: 22/03/2014, 16:20

... oligosaccharides that are fragments of NA2F. The structural information is complemented by thermody- namic parameters of binding determined by means of isothermal titration calorimetry for the above-men- tioned ... 5.2 force field. J Comput Chem 10, 982–1012. Carbohydrate recognition by PAL L. Buts et al. 2420 FEBS Journal 273 (2006) 2407–2420 ª 2006 The Authors Journal compi...

Ngày tải lên: 23/03/2014, 11:20

... diastereomer of Cb-hydroxylated l-arginine. This unexpected stereospecificity is caused by conformational control of the bound sub- strate, which enforces a gauche(–) conformer for v 1 instead of the ... in active site formation by bordering the substrate bound therein. Active site of VioC Crystals of the substrate complex were obtained by crystallization of purified VioC in...

Ngày tải lên: 16/03/2014, 02:20

... code 2DLD for the ternary complex of LDH with oxamate; [14]; PDB code 1J49 for h oloLDH [15]) and D -2-hydroxy- isocaproate dehydrogenase ([16], PDB code 1DXY for the ternary complex of HicDH ... of holoFDH [11], holoPGDH [13], the ternary complex of HicDH [16] and the holo- and ternary complex of LDH ([15], PDB code 2DLD). This orientation directs the Re-hydrogen (pro-R hydro...

Ngày tải lên: 23/03/2014, 13:20

... the carboxylate group of E422 and the amine groups of K43 and K44 of monomer B, and the side chains of R421 and D12 of monomer A. These residues point towards the inte- rior of the peptide binding ... Alternatively spliced exon B of myosin Va is essential for binding the tail-associated light chain shared by dynein. Biochemistry 45, 12582–12595. 3 King SM (2008) Dynein-indep...

Ngày tải lên: 29/03/2014, 09:20

... were examined for growth, +, in the presence of different concentrations of thymidine in the medium. In the last column, LD 100 values are given (in lM) for the growth of KY895 transformed with ... 1. The pattern of inhibition was elucidated by varying dT at four fixed concentrations of dTTP and 2.5 mm ATP, and analyzing the data by the Biosoft (Cambridge, UK) pro- gram enzfi...

Ngày tải lên: 30/03/2014, 20:20

... the loop formation are a of U12, c of T 13 and a of T 14 ,allof which adopt t conformation and thus stretch the backbone. U3-hairpin. The a, b, c and e for each nucleotide in the stem of the U3-hairpin ... both the backbone and the base conformation mimic the B-form of DNA. Thus the structural features of the U2-hairpin provided an explana- tion for its poor excision by th...

Ngày tải lên: 31/03/2014, 09:20