Báo cáo khoa học: Structural basis for recognition of Co2+ by RNA aptamers pot

... 878–892. Recognition of Co2+ by RNA aptamers J. Wrzesinski and S. K. Jo´z´wiakowski1662 FEBS Journal 275 (2008) 1651–1662 ª 2008 The Authors Journal compilation ª 2008 FEBS Structural basis for ... grateful to Professor Jerzy Ciesiolka for critically reading the manuscript and for helpful Recognition of Co2+ by RNA aptamers J. Wrzesinski and S. K. Jo´z´wiakowski1660 FEBS Journal 275 (2008) ... 2008)doi:10.1111/j.1742-4658.2008.06320.xCo2+binding RNA aptamers were chosen as research models to revealthe structural basis underlying the recognition of Co2+ by RNA, with theapplication of two distinct methods. Using...

Tài liệu Báo cáo khoa học: Structural bases for recognition of Anp32⁄LANP proteins doc

... 112–116.Study of the interactions of the Anp32a LRR module C. de Chiara et al.2560 FEBS Journal 275 (2008) 2548–2560 ª 2008 The Authors Journal compilation ª 2008 FEBS Structural bases for recognition of ... a study of the structural determinants for the interactions of Anp32awith other partners. As a prerequisite for binding stud-ies, we ﬁrst solved the structure in solution of Anp32 by NMR spectroscopy. ... The structural information was usedto model the interaction with PP2A, which is knownto be mainly mediated by the PP2A-C subunit. Wesuggest that, by analogy with the mode of recognition of...

Tài liệu Báo cáo khoa học: Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo⁄maltodextrin-binding protein ppt

... positionsand the conformations of the three loops of EcoMBPare different from those of TvuCMBP. Trp62, locatedat region N-I, is found at the center of the curvedform of maltotetraose (Fig. ... that of TvuCMBP (Fig. 7B). Similar protrusions, which areformed by Asp209 and Arg344, are observed at theentrance of the cleft of EcoMBP, but the distancebetween Od2 of Asp209 and Ng2 of Arg344 ... speciﬁcity of thebacterial sugar-binding proteins.Evaluation of the binding afﬁnities by ﬂuorescence measurementsThe Kdvalues of TvuCMBP for binding of sugars weredetermined by measuring...

Báo cáo khoa học: Structural basis for substrate recognition by Erwinia chrysanthemi GH30 glucuronoxylanase potx

... a hypothesis for the basis of sub-strate recognition in this group of so-called ‘append-age-dependent xylanases’ [11]. Examination of thestructure of XynA [14] for the presence of aromaticamino ... wereperformed with leadfinder [23]. This program was alsoused for the preparation of the protein structure for dock-ing by addition of hydrogen atoms according to optimalionization states of protein ... leadfinder.Note added in proofDuring processing of this article for publication wehave learned about the appearence of the paperdescribing similar substrate recognition mechanism by a GH30 xylanase...

Báo cáo khoa học: Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin ppt

... oligosaccharides that are fragments of NA2F. The structural information is complemented by thermody-namic parameters of binding determined by means of isothermal titration calorimetry for the above-men-tioned ... 5.2force ﬁeld. J Comput Chem 10, 982–1012.Carbohydrate recognition by PAL L. Buts et al.2420 FEBS Journal 273 (2006) 2407–2420 ª 2006 The Authors Journal compilation ª 2006 FEBS Structural basis ... position of mannose is drawn in light green, as is thecorresponding orientation of the side chain of Glu221. The second conformation of theside chain of Glu221, which also adopts twoconformations...

Báo cáo khoa học: Structural basis for the erythro-stereospeciﬁcity of the L-arginine oxygenase VioC in viomycin biosynthesis docx

... diastereomer of Cb-hydroxylated l-arginine. This unexpectedstereospeciﬁcity is caused by conformational control of the bound sub-strate, which enforces a gauche(–) conformer for v1instead of the ... inactive site formation by bordering the substrate boundtherein.Active site of VioCCrystals of the substrate complex were obtained by crystallization of puriﬁed VioC in the presence of potassium ... B-factors was justiﬁed for theproduct complexes, owing to the atomic resolution of theirdatasets and a drop of Rfree of more than 2%.Research Collaboratory for Structural Bioinformatics protein...

Báo cáo khoa học: Structural basis for stereo-speciﬁc catalysis in NAD+-dependent (R )-2-hydroxyglutarate dehydrogenase from Acidaminococcus fermentans pptx

... code2DLD for the ternary complex of LDH with oxamate;[14]; PDB code 1J49 for h oloLDH [15]) andD-2-hydroxy-isocaproate dehydrogenase ([16], PDB code 1DXY for the ternary complex of HicDH ... of holoFDH [11], holoPGDH[13], the ternary complex of HicDH [16] and the holo-and ternary complex of LDH ([15], PDB code 2DLD).This orientation directs the Re-hydrogen (pro-R hydro-gen) of ... colour code as for panel A and 2-oxoisocaproate in grey for carbons and red for oxygen atoms. (D) Ste-reo display of the superposition of the activesite for HGDH (colour code as for Fig 4 and2-oxoglutarate...

Báo cáo khoa học: Structural basis for the interaction between dynein light chain 1 and the glutamate channel homolog GRINL1A docx

... the carboxylategroup of E422 and the amine groups of K43 and K44 of monomer B, and the side chains of R421 and D12 of monomer A. These residues point towards the inte-rior of the peptide binding ... Alternativelyspliced exon B of myosin Va is essential for binding thetail-associated light chain shared by dynein.Biochemistry 45, 12582–12595.3 King SM (2008) Dynein-independent functions of DYNLL1 ... Structure of the PIN ⁄ LC8 dimer with a boundpeptide. Nat Struct Biol 6, 735–740.11 Fan J, Zhang Q, Tochio H, Li M & Zhang M (2001) Structural basis of diverse sequence-dependent targetrecognition...

Báo cáo khoa học: Structural basis for the changed substrate speciﬁcity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx

... were examined for growth, +, in the presence of different concentrations of thymidinein the medium. In the last column, LD100values are given (in lM) for the growth of KY895 transformed with ... 1.The pattern of inhibition was elucidated by varying dTat four ﬁxed concentrations of dTTP and 2.5 mm ATP, andanalyzing the data by the Biosoft (Cambridge, UK) pro-gram enzfitter for Windows.CrystallizationThe ... (1996) Functional expression of a fragment of human dihydroorotate dehydrogenase by means of thebaculovirus expression vector system, and kinetic inves-tigation of the puriﬁed recombinant enzyme....

Báo cáo khoa học: Structural basis for poor uracil excision from hairpin DNA An NMR study pptx

... theloop formation are a of U12, c of T13and a of T14,allofwhich adopt t conformation and thus stretch the backbone.U3-hairpin. The a, b, c and e for each nucleotide in thestem of the U3-hairpin ... both the backbone andthe base conformation mimic the B-form of DNA. Thus the structural features of the U2-hairpin provided an explana-tion for its poor excision by the enzyme. However, this stilldid ... 2002 Structural basis for poor uracil excision (Eur. J. Biochem. 269) 1891average. As mentioned earlier, the c of T13and a of T14,arecharacteristically in ÔtÕ conformation. Because of this,...

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