Báo cáo khoa học: The properties of phosphodiesterase 11A4 GAF domains are regulated by modifications in its N-terminal domain pptx
Báo cáo khoa học: The properties of phosphodiesterase 11A4 GAF domains are regulated by modifications in its N-terminal domain pptx
... to the tandem GAF domain of PDE10, and by cGMP when linked to the tandem GAF domain of PDE2, )5or)11. The bio-chemical properties of signalling by human tandem GAF domains to cyaB1 AC were by ... domains preceded by N-terminals of different lengths. GAF domains are small-molecule-binding domains that have been identified in > 3000proteins throughout all taxonomic kingdoms [5,6]. The acronym ... regulating the cGMP affinity of the PDE11A4 tandem GAF domain and thus allosterically affect PDE11 activity. The role of the N-terminus of the PDE11A4 GAF tandem domain The N-termini that precede the...
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Báo cáo khoa học: The occurrence of riboflavin kinase and FAD synthetase ensures FAD synthesis in tobacco mitochondria and maintenance of cellular redox status docx
... pmolÆmg)1protein of Rf being present in the mitochondrial sus-pension. When the FMN concentration was increasedto 50 lm (Fig. 5D), the amount of FAD increasedalmost linearly in the first 10 min of the ... at arate of 413 pmolÆmin)1Æmg)1protein. The amount of FAD reached a maximum value of 3600 pmolÆmg)1protein within 15 min of incubation. Prolonging the incubation time resulted in a significant ... (see above). The amount of FAD wasalso measured enzymatically by using the FAD-detectingsystem, as described in [30,32]. Briefly, the amount of FADwas determined by revealing the reconstituted...
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Báo cáo khoa học: The heterogeneity of mast cell tryptase from human lung and skin Differences in size, charge and substrate affinity ppt
... suggested by differences in staining intensity in lectin binding studies. The lectins SNA/MAA appeared to have a higher affinity for skin tryptasethan for lung tryptase. In contrast, the lectin WGA ... couldconceivably bind by either the P1 or the P3 argininylresidue to the S1 binding pocket. Although binding viaFig. 10. Three-dimensional structure of tryptase [23] indicating positions of potential ... products in Western blots of the lysates of purified lung or skin mast cells and of preparations of tryptase purified from lung or skin tissues. The SEMs are indicated in parenthesis below the mean...
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Báo cáo khoa học: Krit 1 interactions with microtubules and membranes are regulated by Rap1 and integrin cytoplasmic domain associated protein-1 doc
... the interaction, which suggests that the PTB-like F3 sub- domain of the FERM is the C-terminal counterpart.As such, in the dormant form of moesin, the F3 sub- domain is masked by the N-terminal ... activation of integrinsrequires talin binding to the cytoplasmic tail of b integrin [13]. Talin is an essential integrin-activatingprotein that connects the cytoplasmic tail of b inte-grins to the ... binds to PIP2 The molecular modelling of the last 300 amino acidresidues of Krit1 that we generated corroborates the existence of a FERM domain at the C-terminus of the protein. FERM domains...
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Báo cáo khoa học: The importance of polymerization and galloylation for the antiproliferative properties of procyanidin-rich natural extracts doc
... procyanidins are not sig-nificantly absorbed in the intestinal tract, and reach the colon mainly intact [27]. They are therefore bio-available to the epithelial cells in the intestinal wall,where ... colon.Procyanidins and monomeric catechins (Fig. 1) are the main active polyphenols in grape and pine bark. The difference between grape and pine catechins andprocyanidins is found in the presence of gallate ... com-pounds of interest in the prevention of colon cancer. In the present study, we investigated the relationship of different structural factors of procyanidins, such as the mean degree of polymerization...
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Báo cáo khóa học: Structural properties of the protein SV-IV potx
... identified by the massincrease of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species. From the data ... washedaccording to the manufacturer’s instructions. The averageand combined repetitive amino acid yields determined by the instrument software were not lower than 90% for eachsequenced peptide. The theoretical ... of proteins revealing the helical potential. The a-helix content of the monomericform of SV-IV is approximately doubled by the addition of 5.4 mMSDS (Fig. 1), whereas in the 1–70 region, the...
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Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc
... hGBP1) are con-served in the primary structure of the GTPase domain of hGBP5, so the formation of GMP must be impaired in an as yet unknown manner. Sequence alignments of the large GTP-binding domains ... ordering in the proteinduring nucleotide binding. These structural changes were also observed dur-ing changes in the oligomerization state. We observed only a minor in u-ence of the C-terminal ... January2010)doi:10.1111/j.1742-4658.2010.07586.x The human guanylate binding protein 5 (hGBP5) belongs to the family of interferon-c-inducible large GTPases, which are well known for their highinduction by pro -in ammatory cytokines. The...
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Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc
... reduction of protein disulfides o n insulin as substrate; when the inter-chain disulfide bridges are reduced between chains A and B of the insulin, the turbidity of the solution increases because of ... substantiallyfolded protein s ubstrates. Mutations at the first cysteine of the a ctive site in either the N-terminal or C-terminalthioredoxin domain inhibits the capacity of PDI to catalysethiol–disulfide ... using the programme Microsoft Excel 2000. The curve for the determination of the KdforATPwasobtainedusingtheprogramKALEIDA GRAPH3.0.Fig. 5. ATP-binding capacity of PfPDO. Cross-linking of...
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Báo cáo khoa học: Conformational properties of bacterial DnaK and yeast mitochondrial Hsp70 Role of the divergent C-terminal a-helical subdomain pdf
... structures of isolated protein domains [2–6].Thus, Hsp70s are composed of a highly homologous N-terminal ATPase domain of 45 kDa, connected by a short linker to a more variable peptide-binding domain ... westudied the chimeras KKCC and KCCC constructed by domain swapping [18] (Fig. 1A). While maintain-ing the ATPase domain of DnaK, different regions of the more divergent substrate-binding domain wereexchanged: ... 5A), most of the tryptic sites at the C-terminal domain being sensitive to ATP as a consequence of interdomain allosteric coupling. In the absence of nuc-leotide or in the presence of ADP, tryptic...
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Báo cáo khoa học: Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum pdf
... above, hence the tag did not in uence the kin-etic behaviour of the enzyme to any measurable extent.Equilibrium binding affinity of NADH The binding of NADH to the active site of dehydro-genases ... each direc-tion are in keeping with other LDHs.With regard to the nature of the rate-determiningsteps, conformational rearrangement is the predomin-ant kinetic barrier in the single-turnover ... k4 the rate of the product-off step. Hence, in these circumstances, the steady-state KMis expected to be smaller than the apparent KMmeasured in the single turnovers.Furthermore, it is interesting...
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