Báo cáo khoa học: The properties of phosphodiesterase 11A4 GAF domains are regulated by modifications in its N-terminal domain pptx

... to the tandem GAF domain of PDE10, and by cGMP when linked to the tandem GAF domain of PDE2, )5or)11. The bio- chemical properties of signalling by human tandem GAF domains to cyaB1 AC were by ... domains preceded by N-terminals of different lengths. GAF domains are small-molecule- binding domains that have been identified in > 3000 proteins t...

Ngày tải lên: 16/03/2014, 06:20

... pmolÆmg )1 protein of Rf being present in the mitochondrial sus- pension. When the FMN concentration was increased to 50 lm (Fig. 5D), the amount of FAD increased almost linearly in the first 10 min of the ... at a rate of 413 pmolÆmin )1 Æmg )1 protein. The amount of FAD reached a maximum value of 3600 pmolÆmg )1 protein within 15 min of incubation. Prolonging t...

Ngày tải lên: 16/03/2014, 04:20

... suggested by differences in staining intensity in lectin binding studies. The lectins SNA/ MAA appeared to have a higher affinity for skin tryptase than for lung tryptase. In contrast, the lectin WGA ... could conceivably bind by either the P1 or the P3 argininyl residue to the S1 binding pocket. Although binding via Fig. 10. Three-dimensional structure of tryptase [23] i...

Ngày tải lên: 31/03/2014, 07:20

... the interaction, which suggests that the PTB-like F3 sub- domain of the FERM is the C-terminal counterpart. As such, in the dormant form of moesin, the F3 sub- domain is masked by the N-terminal ... activation of integrins requires talin binding to the cytoplasmic tail of b integrin [13]. Talin is an essential integrin-activating protein that connects the c...

Ngày tải lên: 16/03/2014, 05:20

... procyanidins are not sig- nificantly absorbed in the intestinal tract, and reach the colon mainly intact [27]. They are therefore bio- available to the epithelial cells in the intestinal wall, where ... colon. Procyanidins and monomeric catechins (Fig. 1) are the main active polyphenols in grape and pine bark. The difference between grape and pine catechins and procyani...

Ngày tải lên: 07/03/2014, 05:20

... identified by the mass increase of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species. From the data ... washed according to the manufacturer’s instructions. The average and combined repetitive amino acid yields determined by the instrument software were not lower t...

Ngày tải lên: 07/03/2014, 14:20

... hGBP1) are con- served in the primary structure of the GTPase domain of hGBP5, so the formation of GMP must be impaired in an as yet unknown manner. Sequence alignments of the large GTP-binding domains ... ordering in the protein during nucleotide binding. These structural changes were also observed dur- ing changes in the oligomerization state. We observed o...

Ngày tải lên: 15/03/2014, 10:20

... reduction of protein disulfides o n insulin as substrate; when the inter- chain disulfide bridges are reduced between chains A and B of the insulin, the turbidity of the solution increases because of ... substantially folded protein s ubstrates. Mutations at the first cysteine of the a ctive site in either the N-terminal or C-terminal thioredoxin domain inhibits...

Ngày tải lên: 16/03/2014, 16:20

... structures of isolated protein domains [2–6]. Thus, Hsp70s are composed of a highly homologous N-terminal ATPase domain of  45 kDa, connected by a short linker to a more variable peptide-binding domain ... we studied the chimeras KKCC and KCCC constructed by domain swapping [18] (Fig. 1A). While maintain- ing the ATPase domain of DnaK, different regions of th...

Ngày tải lên: 16/03/2014, 22:20

... above, hence the tag did not in uence the kin- etic behaviour of the enzyme to any measurable extent. Equilibrium binding affinity of NADH The binding of NADH to the active site of dehydro- genases ... each direc- tion are in keeping with other LDHs. With regard to the nature of the rate-determining steps, conformational rearrangement is the predomin- ant kinetic...

Ngày tải lên: 23/03/2014, 09:20