Báo cáo khoa học: Ubiquitination of E3 ubiquitin ligase TRIM5a and its potential role pdf

... (lanes 3 and 8) A B C Fig. 5. In vivo assay of self -ubiquitination and cross -ubiquitination between Ro52 and TRIM5a. (A) In vivo ubiquitination by Ro52 E3 ubiquitin ligase. To examine the ubiquitination ... UnpEL(C311A), Fig. 6. Ubiquitination of TRIM5a by TRIM5a and Ro52: a compara- tive study. To compare the ligase activities of TRIM5a and Ro52 for th...

Ngày tải lên: 16/03/2014, 06:20

... chromosome 3p, mutations of the VHL suppressor gene and loss of heterozygos- ity (LOH) of 9p, 14q, 17p and 10q have been reported in cRCC [4]. Trisomy 7 and 17 and loss of the Y-chromosome are ... [4], and, despite the good prog- nosis of chRCC, loss of various chromosomes, muta- tions of p53, LOH at 10q23.3 and telomere shortening have been reported [6]. RO consists...

Ngày tải lên: 06/03/2014, 22:21

... wavelength of 430 nm and an excitation wavelength of 330 nm for HP and at an emission wavelength of 446 nm and an excitation wavelength of 360 nm for HPO. The emission and excita- tion slits were ... Fluorescence traces for NP, MP and HPO were obtained with an excitation wavelength of 360 nm and a filter with a cutoff of 395 nm for emission. Mechanism and kinetics of...

Ngày tải lên: 19/02/2014, 00:20

... cuvette and a protein concentration of 19 lm. Tryptophan emission spectra were obtained using an excitation wavelength of 280 nm, with excitation and emission slits of 3 nm, and a scan rate of 60 ... analysis of ligand binding to C-LytA. (A) Con- formational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands:...

Ngày tải lên: 19/02/2014, 05:20

... 6, 10, 14, 18, 22, 26, and 30), 2 l M (lanes 3, 7, 11, 15, 19, 23, 27, and 31), and 4 lM (lanes 4, 8, 12, 16, 20, 24, 28, and 32). Y. Matsuo et al. Roles of the HsRad51 L1 and L2 loops FEBS Journal ... 375–380. Y. Matsuo et al. Roles of the HsRad51 L1 and L2 loops FEBS Journal 273 (2006) 3148–3159 ª 2006 The Authors Journal compilation ª 2006 FEBS 3159 Roles of the human Rad...

Ngày tải lên: 19/02/2014, 06:20

... cloning and characterization of a gene encoding the soluble PPase of the roundworm Ascaris suum. The predicted A. suum PPase consists of 360 amino acids with a molecular mass of 40.6 kDa and a pI of ... the kinetics and properties of the enzyme. More strikingly, we show a novel role of the PPase enzyme in the development and molting process of A. suum larvae in vitro...

Ngày tải lên: 20/02/2014, 11:20

... combination of untagged and His-tagged subunits, evidence was obtained for a complex formation between PecE and PecF (subunits of PVB-PEC- lyase), and by experiments with single subunits for the ... around 470 nm; and in the presence of Ni 2+ , a broad, unstructured absorption formed in the 610–650 nm region. Both Mn 2+ and Mg 2+ are ubiquitous chelators of nucleotides and...

Ngày tải lên: 21/02/2014, 03:20

... injection of the toxin CagA. We review the progress made in the past decade in our understanding of T4SS architecture. We translate this new knowledge to derive an understanding of the structure of ... pylori; see the accompanying reviews by Fischer [21] and Cendron and Zanotti [22]. The role of most Structure of the Cag-T4SS L. Terradot and G. Waksman 1214 FEBS Journal 27...

Ngày tải lên: 06/03/2014, 00:21

... C, H, N, S and Ca 2+ contents, and density of purified InsP 6 deposits. InsP 6 deposits were purified by alkaline hydrolysis of the Echinococcus granulous cyst wall followed by washing and drying ... cm 2 portion of LL, of typical 1 mm thickness, comprises some 4000 cm 2 of InsP 6 deposit surface area. Even if, because of granule coales- cence and interaction between granule...

Ngày tải lên: 07/03/2014, 12:20

... interconversions and inhibition of biosynthetic processes after exposure of L1210 leukemia cells to antifolates. Experimental and network thermodynamic analyses of the role of dihydro- folate ... Ciudad Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, Spain Methotrexate (MTX) is a 4-amino 10-methyl analog of folic acid that...

Ngày tải lên: 07/03/2014, 16:20