Báo cáo khoa học: Ubiquitination of E3 ubiquitin ligase TRIM5a and its potential role pdf

... (lanes 3 and 8)ABCFig. 5. In vivo assay of self -ubiquitination and cross -ubiquitination between Ro52 and TRIM5a. (A) In vivo ubiquitination by Ro52 E3 ubiquitin ligase. To examine the ubiquitination ... UnpEL(C311A),Fig. 6. Ubiquitination of TRIM5a by TRIM5a and Ro52: a compara-tive study. To compare the ligase activities of TRIM5a and Ro52for the ubiquitination of TRIM5a, anin vivo ubiquitination ... detect both nonubiquitinated and ubi-quitinated MBP TRIM5a (lanes 1 and 2), and antibody to RH to detect ubiquitinated MBP TRIM5a (lanes 3 and 4). Ubiquitination of TRIM5a and its role K. Yamauchi...

Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf

... chromosome 3p, mutations of the VHL suppressor gene and loss of heterozygos-ity (LOH) of 9p, 14q, 17p and 10q have beenreported in cRCC [4]. Trisomy 7 and 17 and loss of the Y-chromosome are ... [4], and, despite the good prog-nosis of chRCC, loss of various chromosomes, muta-tions of p53, LOH at 10q23.3 and telomereshortening have been reported [6]. RO consists of mixtures of cells ... [24,25] and of butyrylcholinesterase itselfin megakaryocytopoiesis suppression and retinal celldifferentiation [16].The expression of acetylcholinesterase and butyr-ylcholinesterase in neural and...

Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx

... wavelength of 430 nm and an excitation wavelength of 330 nm for HP and at an emission wavelength of 446 nm and an excitationwavelength of 360 nm for HPO. The emission and excita-tion slits were ... Fluorescence traces for NP, MP and HPO were obtained with an excitation wavelength of 360 nm and a ﬁlter with a cutoff of 395 nm for emission.Mechanism and kinetics of dihydroneopterin aldolase ... viathe deprotonation and re-protonation of C2¢ and report a comprehensive equilibrium and kinetic study of SaDHNA and EcDHNA, which represent DHNAsfrom Gram-positive and Gram-negative bacteria,respectively....

Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx

... cuvette and aprotein concentration of 19 lm. Tryptophan emissionspectra were obtained using an excitation wavelength of 280 nm, with excitation and emission slits of 3 nm, and ascan rate of 60 ... analysis of ligand binding to C-LytA. (A) Con-formational changes induced by ligands on C-LytA monitored bynear-UV CD with no ligand added (—) and upon addition of ligands:s, d, 2.5 and 20 mM ... near-UV CD spectrum of C-LytA at 20 °C and pH 7.0, showing two maxima at265 nm and 290 nm. Upon addition of 20 mm choline(a saturating concentration of ligand), two minima at284 nm and 294 nm appear,...

Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc

... 6, 10, 14, 18, 22, 26, and 30), 2 lM (lanes 3, 7, 11, 15, 19, 23, 27, and 31), and 4 lM (lanes 4, 8, 12, 16, 20, 24, 28, and 32).Y. Matsuo et al. Roles of the HsRad51 L1 and L2 loopsFEBS Journal ... 375–380.Y. Matsuo et al. Roles of the HsRad51 L1 and L2 loopsFEBS Journal 273 (2006) 3148–3159 ª 2006 The Authors Journal compilation ª 2006 FEBS 3159Roles of the human Rad51 L1 and L2 loops in DNA ... HsRad51-L1 loop and measured the ﬂuorescence changes of the tryptophanresidues upon DNA binding.ResultsStrategy of mutational analysisIn order to study the functions of the L1 and L2loops of HsRad51,...

Tài liệu Báo cáo khoa học: Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites doc

... cloning and characterization of a geneencoding the soluble PPase of the roundworm Ascarissuum. The predicted A. suum PPase consists of 360 aminoacids with a molecular mass of 40.6 kDa and a pI of ... the kinetics and properties of theenzyme. More strikingly, we show a novel role of the PPaseenzyme in the development and molting process of A. suumlarvae in vitro.Materials and methodsParasitesAdult ... (control) and presence of increasing concentrations of inhibitors for10 days, and the number of molting larvae was determined.Molting was manifested by shedding of the L3 cuticle.Numbers of molted...

Tài liệu Báo cáo khoa học: Characterization of phycoviolobilin phycoerythrocyanin-a84-cysteinlyase-(isomerizing) from Mastigocladus laminosus pdf

... combination of untagged and His-tagged subunits, evidence was obtained for a complexformation between PecE and PecF (subunits of PVB-PEC-lyase), and by experiments with single subunits for the ... around 470 nm; and in thepresence of Ni2+, a broad, unstructured absorption formedin the 610–650 nm region.Both Mn2+ and Mg2+are ubiquitous chelators of nucleotides and cofactors of many related ... after the other [31,32].Action of individual subunits, PecE and PecFAs shown before, both lyase subunits, PecE and PecF, arenecessary for the reconstitution of PCB and PecA to yieldFig. 3. His6-PecE/F...

Báo cáo khoa học: Architecture of the Helicobacter pylori Cag-type IV secretion system pdf

... injection of the toxin CagA. Wereview the progress made in the past decade in our understanding of T4SSarchitecture. We translate this new knowledge to derive an understanding of the structure of ... pylori; see the accompanying reviews by Fischer[21] and Cendron and Zanotti [22]. The role of mostStructure of the Cag-T4SS L. Terradot and G. Waksman1214 FEBS Journal 278 (2011) 1213–1222 ... translocation pore of the Cag-T4SSUntil recently, the periplasmic core of the T4SS wasconsidered to be composed of VirB8, VirB7, VirB9 and VirB10. The structures of these proteins or parts of these proteins...

Báo cáo khoa học: Characterization of myo-inositol hexakisphosphate deposits from larval Echinococcus granulosus pdf

... C, H, N, S and Ca2+contents, and density of puriﬁed InsP6deposits. InsP6deposits were puriﬁed by alkaline hydrolysis of theEchinococcus granulous cyst wall followed by washing and drying ... cm2portion of LL, of typical1 mm thickness, comprises some 4000 cm2 of InsP6deposit surface area. Even if, because of granule coales-cence and interaction between granules and LL ﬁbrils,part of ... in supernatant, and the absorbance(A) at 240 nm (indicative of b-elimination of O-glycosylated serine and threonine residues). Carbohydrates were estimated as the sum of masses of galactose, N-acetylgalactosamine...

Báo cáo khoa học: Modulation of IMPDH2, survivin, topoisomerase I and vimentin increases sensitivity to methotrexate in HT29 human colon cancer cells docx

... interconversions and inhibition of biosynthetic processes after exposure of L1210 leukemia cells to antifolates. Experimental and network thermodynamic analyses of the role of dihydro-folate ... CiudadDepartment of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, SpainMethotrexate (MTX) is a 4-amino 10-methyl analog of folic acid that inhibits dihydrofolate reductase(DHFR), ... Pharmacology and mechanism of action of pemetrexed. Clin Lung Cancer 5 (Suppl. 2), S51–S55.27 Longo-Sorbello GS & Bertino JR (2001) Current under-standing of methotrexate pharmacology and efﬁcacy...

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