Báo cáo khoa học: Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4) potx

... Journal compilation ª 2008 FEBS Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4) Fumitaka Ichioka, Ryota Kobayashi, ... cysteine, A for generally aliphatic aminoacid, and X for any amino acid). Mammalian Alix and its yeast ortholog, Bro1, are known to associate with charged multivesicular body protein 4 (CHMP4), a component ... phosphor-ylation of Alix ⁄ AIP1 modulates its interaction with binding partners and antagonizes its activities. J BiolChem 280, 341 4– 342 5.13 Shibata H, Yamada K, Mizuno T, Yorikawa C,Takahashi H, Satoh...

Tài liệu Báo cáo khoa học: TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses pdf

... AGCTGATCTTCGAAGATCTTCGAAGATMutated HSEsenseBiotin-TCGACTTCAAGCTTGTACAAGCTTGTAGMutated HSEantisenseAGCTGAAGTTCGAACATGTTCGAACATC‘Scrambled’oligonucleotideBiotin-AACGACGGTCGCTCCGCCTGGCT 140 40 6080100120Counts ... TransLISA, a novel quantitative, nonradioactive assayfor transcription factor DNA-binding analysesKristiina A. Vuori1, Johanna K. Ahlskog2, Lea Sistonen2and Mikko Nikinmaa11 Centre ... plate,and acceptor beads containing antibody against HSF1 are added to the wells. After incubation, streptavidin-coated donor beads are added,and plates are covered and incubated at room temperature...

Tài liệu Báo cáo khóa học: TbPDE1, a novel class I phosphodiesterase of Trypanosoma brucei pdf

... 5¢-GGGAATTCCATATGAGAGACAATATTTCCCGTTTATCAAATC-3¢ and 5¢-CCGCTCGAGTCATTACTAGGTTCCCTGTCCAGTGTTACC-3¢,andPDE1(Lys321–Thr620) was ampliﬁed with primers 5¢-GGGAATTCCATATGAAGAATGATCAATCTGGCTGCGGCGCAC-3¢ and 5¢-CCGCTCGAGTCATTACTAGGTTCCCTGTCCAGTGTTACC-3¢. ... (accessnumber AAC5 043 7); 2, human PDE 2A (O0 040 8); 3, human PDE 3A (AAA35912); 4, human PDE 4A (AAC35012); 5, human PDE 5A (NM_0010 74) ; 6, human PDE6B (NP_0002 74) ; 7, human PDE 7A (Q13 946 ); 8, human PDE 8A (O60658); ... The African trypanosomeTrypanosoma brucei is the protozoon that causes the fatalhuman sleeping sickness, as well as Nagana, a devastatingdisease of domestic animals in large parts of sub-SaharanAfrica....

Báo cáo khoa học: Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus pdf

... show vast differences in p reference f or insect andmammalian Na+channels. Accordingly, they are dividedinto classical a- toxins that are highly active in mammalianbrain, a- toxins that are ... from poly (A) + mRNA u sing M-MLV reverse tran-scriptase. The cDNA was joined with the a daptor providedby the kit (5 ¢-gcugauggcgaugaaugaacacugcguuugCUGGCUUUGAUGAAA-3¢) using T4 DNA ligase. The ... Biotecnologı´aUNAMAvenida Universidad, 2001 Apartado Postal 510–3 Cuernavaca 62210Mexico. Fax: +52 777 3172388, Tel.: + 52 777 3171209,E-mail: possani@ibt.unam.mxAbbreviations: a. m.u., atomic mass...

Báo cáo khoa học: Plasmoredoxin, a novel redox-active protein unique for malarial parasites doc

... Hatabu, T., Kawai, S., Matsumoto, Y. &Kano, S. (2000) Molecular cloning and characterization of a peroxiredoxin from the human malaria parasite Plasmodium fal-ciparum. Mol. Biochem. Parasitol. ... falciparum.Thisresult, and the fact that PfPlrx was ampliﬁed from a cDNAlibrary, indicate that the gene is transcribed and the protein is present in blood-stage forms of the parasite that causemalaria ... structural and functional characteristics. In the mal-arial parasite, Plasmodium falciparum, a functional thio-redoxin and glutathione system have been demonstratedand are considered to be attractive...

Báo cáo khoa học: CmtR, a cadmium-sensing ArsR–SmtB repressor, cooperatively interacts with multiple operator sites to autorepress its transcription in Mycobacterium tuberculosis pptx

... 2P2R AGCAAGAGCTGAATTGTACATP3F GCTGTTATACCAGTATATGG EMSA,oligonucleotidesfor site 3P3R CCATAT ACTGGTATAACAGCP4F TGGTGTACTAATTTGATCTATG EMSA,oligonucleotidesfor site 4 P4R CATAGATCAAATAGTACACCAH1 ... Reporter assay,EMSA, DNaseI footprintingP8 TGTTATACCAGTATATGGTGTACTA EMSA94RTf CTCGGCCTCAACTACAGTCGT Reverse transcription94RTr ACAGGTAGCTGAGCAGCAGAC Reverse transcription1994intR CAGCTAGCTGGCCGGGATAGC ... ExperimentP1 GTACTATTGGATCCATGCTGACG CmtR protein expressionP2 GTCCTGTAAGCTTAAGTCGTGTC CmtR protein expressionP3 TTCCCGCATCTCACACGTCA Reporter assayP4 CATATCTGCTATGGATGTAC EMSAP6 GTCACACCTTTCGTCGCAGC...

Báo cáo khoa học: "Generating a Non-English Subjectivity Lexicon: Relations That Matter" ppt

... (deﬁnitions).Nodes and arcs of such a graph are assignedweights, which are then propagated through thegraph by iteratively applying a PageRank-like al-gorithm.Initially, weights are assigned to nodes and arcsin ... provides a more ﬂexible experimentalframework for applications that will use the lexi-con.Esuli and Sebastiani (Esuli and Sebastiani,2007) apply an algorithm based on PageRank torank synsets ... to evaluate the performance of ranking clas-siﬁers. The AUC value corresponds to the proba-bility that a randomly drawn positive instance willbe ranked higher than a randomly drawn negativeinstance....

Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx

... K90T (a ﬁ c), C 140 S (t ﬁ a) , Q 14 3A (cag ﬁ gcg) DS- 14 K44R (a ﬁ g), C 140 S (t ﬁ a) , Q 14 3A (cag ﬁ gcg)DS-20 L60 (t ﬁ c), N73S (a ﬁg), Q 14 3A (cag ﬁ gcg)Table 2. Steady-state constants and rate constant ... N73S–C 140 S–Q 14 3A hMnSOD is essentially the same as that of N73S–Q 14 3A hMnSOD.Cys 140 has its Ca located 12.5 A ˚from the mangan-ese, with its side chain pointing away from the man-ganese and ... a) ,Q 14 3A (cag ﬁ gcg), E187Q (g ﬁ c)DS-2 C 140 S (t ﬁ a) , Q 14 3A (cag ﬁ gcg)EP-15 A5 0V (c ﬁ t), C 140 S (t ﬁ a) , Q 14 3A (cag ﬁ gcg) DS-9 L 14 (g ﬁ a) , N73S (a ﬁ g), Q 14 3A (cag ﬁ gcg)EP -40 C 140 S (t ﬁ a) ,...

Báo cáo khoa học: Weak toxin WTX from Naja kaouthia cobra venom interacts with both nicotinic and muscarinic acetylcholine receptors potx

... Polyclonal antibodies against nativeweak toxin Naja kaouthia discriminate native weaktoxins and some other three-ﬁngered toxins againsttheir denaturated forms. Toxicon 46 , 24 30. 40 Evenas J, Malmendal ... cycle with food andwater ad libitum.Membrane preparationsAdrenal membranes were obtained as described previously [44 ]. The procedure was performed in a cold room, and alllabware was washed with ... G-proteins with the M1,M 4 , and M5subtypes.Our data demonstrate an orthosteric WTX interaction with nAChR andan allosteric interaction with mAChRs.AbbreviationsACh, acetylcholine; AChR, acetylcholine...

Báo cáo khoa học: The A domain of ﬁbronectin-binding protein B of Staphylococcus aureus contains a novel ﬁbronectin binding site pdf

... GGGGGATCCGGTACAGATGTAACAAATAAAG BamHIrFnBPB163 46 3R ATTCCCGGGTAATTTTTCCAAGTTAAATTACTTG SmaIrFnBPB163–308F GGGGGATCCGGTACAGATGTAACAAATAAAG BamHIrFnBPB163–308R CTCCCCGGGCTATTGAATATTAAATATTTTGCTAA ... GAATTATCTTTAGCTCTAGCTATTGATCCrFnBPB163 48 0NF F GGATCAATAGCTAGAGCTAAAGATAATTCFnBPB(– 142 48 0)F GCAGAATTCGTCGGCTTGAAATACGCTG EcoRIFnBPB(– 142 48 0)R AATGGATCCTTACTTTAGTTTATCTTTGCCG BamHIFnBPB(388–980)F CCCAAGCTTGATGATGTCAGC Hind ... CTCCCCGGGCTATTGAATATTAAATATTTTGCTAA SmaIrFnBPB309 48 0F CCCGGATCCTATTTAGGTGGAGTTAGAGATAAT BamHIrFnBPB309 48 0R AATCCCGGGTTACTTTAGTTTATCTTTGCCG SmaIrFnBPB163 48 0NF F GAATTATCTTTAGCTCTAGCTATTGATCCrFnBPB163 48 0NF...

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