Báo cáo khoa học: Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4) potx

... Journal compilation ª 2008 FEBS Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4) Fumitaka Ichioka, Ryota Kobayashi, ... cysteine, A for generally aliphatic amino acid, and X for any amino acid). Mammalian Alix and its yeast ortholog, Bro1, are known to associate with charged m...

Ngày tải lên: 16/03/2014, 06:20

... AGCTGATCTTCGAAGATCTTCGAAGAT Mutated HSE sense Biotin-TCGACTT CAAGCTTGTACAAGCTTGTAG Mutated HSE antisense AGCTGAAGTTCGAACATGTTCGAACATC ‘Scrambled’ oligonucleotide Biotin-AACGACGGTCGCTCCGCCTGGCT 140 40 60 80 100 120 Counts ... TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses Kristiina A. Vuori 1 , Johanna K. Ahlskog 2 , Lea Sistonen 2 a...

Ngày tải lên: 18/02/2014, 14:20

... 5¢-GGGAATTCCATATGAGAGACAATA TTTCCCGTTTATCAAATC-3¢ and 5¢-CCGCTCGAGT CATTACTAGGTTCCCTGTCCAGTGTTACC-3¢,and PDE1(Lys321–Thr620) was amplified with primers 5¢-GGG AATTCCATATGAAGAATGATCAATCTGGCTGCG GCGCAC-3¢ and 5¢-CCGCTCGAGTCATTACTAGG TTCCCTGTCCAGTGTTACC-3¢. ... (access number AAC5 043 7); 2, human PDE 2A (O0 040 8); 3, human PDE 3A (AAA35912); 4, human PDE 4A (AAC35012); 5, human PDE 5A (N...

Ngày tải lên: 19/02/2014, 12:20

... show vast differences in p reference f or insect and mammalian Na + channels. Accordingly, they are divided into classical a- toxins that are highly active in mammalian brain, a- toxins that are ... from poly (A) + mRNA u sing M-MLV reverse tran- scriptase. The cDNA was joined with the a daptor provided by the kit (5 ¢-gcugauggcgaugaaugaacacugcguuugCUGG CUUUGAUGAAA-3¢) using T4 DNA...

Ngày tải lên: 07/03/2014, 16:20

... Hatabu, T., Kawai, S., Matsumoto, Y. & Kano, S. (2000) Molecular cloning and characterization of a peroxiredoxin from the human malaria parasite Plasmodium fal- ciparum. Mol. Biochem. Parasitol. ... falciparum.This result, and the fact that PfPlrx was amplified from a cDNA library, indicate that the gene is transcribed and the protein is present in blood-stage forms of the parasi...

Ngày tải lên: 31/03/2014, 07:20

... 2 P2R AGCAAGAGCTGAATTGTACAT P3F GCTGTTATACCAGTATATGG EMSA, oligonucleotides for site 3 P3R CCATAT ACTGGTATAACAGC P4F TGGTGTACTAATTTGATCTATG EMSA, oligonucleotides for site 4 P4R CATAGATCAAATAGTACACCA H1 ... Reporter assay, EMSA, DNase I footprinting P8 TGTTATACCAGTATATGGTGTACTA EMSA 94RTf CTCGGCCTCAACTACAGTCGT Reverse transcription 94RTr ACAGGTAGCTGAGCAGCAGAC Reverse transcription 1994intR...

Ngày tải lên: 23/03/2014, 04:21

... (definitions). Nodes and arcs of such a graph are assigned weights, which are then propagated through the graph by iteratively applying a PageRank-like al- gorithm. Initially, weights are assigned to nodes and arcs in ... provides a more flexible experimental framework for applications that will use the lexi- con. Esuli and Sebastiani (Esuli and Sebastiani, 2007) apply an algorithm base...

Ngày tải lên: 24/03/2014, 03:20

... K90T (a fi c), C 140 S (t fi a) , Q 14 3A (cag fi gcg) DS- 14 K44R (a fi g), C 140 S (t fi a) , Q 14 3A (cag fi gcg) DS-20 L60 (t fi c), N73S (a fi g), Q 14 3A (cag fi gcg) Table 2. Steady-state constants and rate constant ... N73S– C 140 S–Q 14 3A hMnSOD is essentially the same as that of N73S–Q 14 3A hMnSOD. Cys 140 has its Ca located 12.5 A ˚ from the mangan- ese, with its sid...

Ngày tải lên: 07/03/2014, 11:20

... Polyclonal antibodies against native weak toxin Naja kaouthia discriminate native weak toxins and some other three-fingered toxins against their denaturated forms. Toxicon 46 , 24 30. 40 Evenas J, Malmendal ... cycle with food and water ad libitum. Membrane preparations Adrenal membranes were obtained as described previously [44 ]. The procedure was performed in a cold room, and all lab...

Ngày tải lên: 23/03/2014, 05:22

... GGGGGATCCGGTACAGATGTAACAAATAAAG BamHI rFnBPB 163 46 3 R ATTCCCGGGTAATTTTTCCAAGTTAAATTACTTG SmaI rFnBPB 163–308 F GGGGGATCCGGTACAGATGTAACAAATAAAG BamHI rFnBPB 163–308 R CTCCCCGGGCTATTGAATATTAAATATTTTGCTAA ... GAATTATCTTTAGCTCTAGCTATTGATCC rFnBPB 163 48 0 NF F GGATCAATAGCTAGAGCTAAAGATAATTC FnBPB (– 142 48 0) F GCAGAATTCGTCGGCTTGAAATACGCTG EcoRI FnBPB (– 142 48 0) R AATGGATCCTTACTTTAGTTTATCTTTGC...

Ngày tải lên: 06/03/2014, 00:20