Báo cáo khoa học: Characterization of the glutamyl endopeptidase from Staphylococcus aureus expressed in Escherichia coli pptx

... nonheated samples, the mature form of GluV8 could be renatured even after exposure to heat in the presence of SDS. Role of N-terminal Val69 in processing of the GluV8 proform Finally, the role of N-terminal ... for the correct folding of GluV8, indicating its role as an intramolecular chap- erone. Furthermore, the four amino acid residues at the C-terminus of...

Ngày tải lên: 16/03/2014, 06:20

... analysis of fractions obtained during subsequent steps of c-TMT purification from Capsicum fruits. From each step, 1 lg of protein was loaded on the gel. Proteins were visualized by silver staining. The ... 4A). Comparison of the properties of the partially purified c-TMT from Capsicum fruits with the recombinant c-TMT from Arabidopsis Recombinant Arabidopsis c-TMT c...

Ngày tải lên: 17/03/2014, 09:20

... with the first 88 amino acids of PPI1 [22], indicating that the PM H + -ATPase binding site of PPI1 is localized therein. Indeed, fusion proteins containing the first 88 amino acids of PPI1, linked ... suggested by the parallel increase of the number of FC-binding sites [18–20]. As to auxin, a soluble auxin receptor has been reported to bind and activate the PM H + -ATPase...

Ngày tải lên: 19/02/2014, 07:20

... of the genes. In SNO1, the 199th G from the 5¢ terminus of the open reading frame was deleted to result in a frame-shift and appearance of stop codons in the downstream. As a result, a protein ... or two of the recombinant plasmids. The resulting cells over -expressed the desired protein(s) up to 15% of the total cellular proteins. When Sno1p was expresse...

Ngày tải lên: 19/02/2014, 12:20

... binding factors. The introduction of mutation in one of the Ets-binding sequences greatly decreased its promoter activity, suggesting that the transcription of the a3 integrin gene in these cells ... including Ets, GATA, and MyoD/E-box binding factors. The introduction of mutation into one of the putative Ets- binding sequences suppressed the promoter activity. In...

Ngày tải lên: 21/02/2014, 03:20

... suggested a single cut at the C9–C10 double bond of the ring-bearing side of 3-OH-c-carotene in combination with several cleavage options in the linear half of the molecule, namely at the C9¢–C10¢,C7¢–C8¢ ... temperature of 340 °C, which was maintained for an addi- tional 5 min. The He carrier gas flow was maintained at 1mLÆmin )1 using a split flow of 1 : 20. The sp...

Ngày tải lên: 07/03/2014, 03:20

... arrows). Owing to the horizontal smear, however, the presence of the B14 subunit in the lower band cannot be ascer- tained at this point. A cross-reaction of the antibody against the 39 kDa subunit of ... Duve Institute of Cellular Pathol- ogy) for critical reading of the manuscript. This work was supported by grants from the Grant Agency of the Ministry of...

Ngày tải lên: 07/03/2014, 09:20

... the N ter- minus of ribosomal protein L11 in regulation of the RelA protein of Escherichia coli. J Bacteriol 183, 6532– 6537. 11 Thompson J, Cundliffe E & Stark M (1979) Binding of thiostrepton ... [7]. tRNA-dependence of pppGpp synthesis Binding states of tRNAs It was important to determine the binding states of tRNAs in ribosomal complexes that stimulated pppG...

Ngày tải lên: 07/03/2014, 16:20

... Norway Haemerythrin proteins comprise a family of O 2 -carrry- ing proteins mainly found in a few phyla of marine invertebrates. Members of this family differ from haemoglobin and haemocyanin in that they ... probe made from the PCR fragment amplified with the primers used in the cloning of MCA0715. The arrowhead indicates the 540 bp transcript, corresponding to th...

Ngày tải lên: 07/03/2014, 17:20

... structure. The confor- mations of residues 8–9 are ill defined in the peptides. Thus, the introduction of a prolinoamino acid in the C-terminal tail of SP does not affect the overall structure of the ... space beside the absence of c-turn minima. Discussion The nonsubstituted proline analogue [Pro11]SP is only a weak competitor of SP binding sites. Reintroducing...

Ngày tải lên: 08/03/2014, 02:21