Báo cáo khoa học: Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures potx

... 19 REVIEW ARTICLE Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures Alexander Wlodawer 1 , Wladek Minor 2,3 , Zbigniew ... or processing error in the PDB. Another resolution-related problem is whether indi- vidual B-factors were refined, or whether only an overall B (for...

Ngày tải lên: 16/03/2014, 06:20

... with the formal query which would formulate in terms of the actual data base structure what the desired information is. An important decision in the design of a q.a. system is therefore, how to ... This may seem to force us to give up local translation for certain cases: instead, we would have to design an algorithm which looks out for sub-expressions of the form...

Ngày tải lên: 21/02/2014, 20:20

... than the ones reported here for the new system. 5 The results for different word classes are not directly com- parable because they are affected by the total number of SCFs evaluated for each word ... with the size of the input data to acquisition (the more data, the better perfor- mance). When considering the F-measure results for the individual words shown in...

Ngày tải lên: 17/03/2014, 04:20

... . Initialize Set Set For all , set . Set For , calculate – – – Repeat for = 1 to Choose Set Update one parameter, for – – – for , – for , – for – – – for , – for , – For all features whose values of and /or have ... features of the words before/after the start of the entity (16 features total, only 4 shown) TO0 00= TO1 11= TO2 000= TO2 111= Trigram boundary fe...

Ngày tải lên: 17/03/2014, 08:20

... 50 l M SB-415286 for 60 min prior to incubation with insulin (1 n M or 100 n M ) for 10 min (for phospho-blots) or for 30 min (uptake assays). Cells were lysed and 50 lglysateproteinwas immunoblotted ... pretreated for 5 min with 100 n M wortmannin, 10 l M SB-203580, 10 l M PD-98059 or 10 l M rapamycin prior to cell stimulation with 100 n M insulin for 30 min or 50 m M...

Ngày tải lên: 08/03/2014, 08:20

... affinities for sugars but of whether the protein adopts the open form or the closed form. TvuCMBP–c-CD complex is seen as the closed form (Fig. 2A,B), and this struc- ture shows that the sugar-binding ... cleft formed by the two domains. A common functional conformational change has been reported in this protein family, which involves switching from an open form to a su...

Ngày tải lên: 19/02/2014, 00:20

... scaffold proteins interact with p38 MAPK isoforms, how these interactions are regu- lated, how they interplay with catalysis, how they may be exploited therapeutically or how they differ within the ... the inhibitor displays selectivity towards certain enzyme forms. We believe the uncompetitive pattern towards ATF2D115 (no slope effect) results because MgADP does not bind t...

Ngày tải lên: 16/03/2014, 22:20

... loved one. Once life- supporting care is instituted, the patient has options for ‘survival’ that they did not have before, even though they are dependent on ‘life support’. There are now variables Commentary Medical ... their authority to make choices extends to making bad ones. 2. Moribund patients look comfortable on ‘life support’. An observer’s primal reaction to the vibrant...

Ngày tải lên: 25/10/2012, 10:45

... A ˚ 2 for main-chain atoms, 35.7 A ˚ 2 for side-chain atoms and 33.8 A ˚ 2 overall (3244 atoms). For the pep- tide, B factors are slightly higher than those for importin-a: 36.9 A ˚ 2 for main-chain ... according to the following equation: B z ¼ B i À l j r j where B z is the normalized B factor, B i is the B factor of atom i, l j is the mean B factor (excluding water mo...

Ngày tải lên: 14/02/2014, 19:20

... were converted to their corre- sponding alditols before MS acquisition. The other types of glycan stored in the GMDB are mostly tagged with 2-aminopyridine, which can be used for fluo- rescence ... on the principle of evanescent-field fluorescence detection (Fig. 3A). Furthermore, they succeeded in improving the array platform analysis to achieve the highest sensitivity reported...

Ngày tải lên: 16/02/2014, 08:20