Báo cáo khoa học: Effects of the G376E and G157D mutations on the stability of yeast enolase – a model for human muscle enolase deficiency pdf

... Journal 275 (2008) 9 7–1 06 ª 2007 The Authors Journal compilation ª 2007 FEBS Effects of the G376E and G157D mutations on the stability of yeast enolase – a model for human muscle enolase deficiency Songping ... believe that the effects of these mutations on the structure and function of yeast enolase will be similar to their...

Ngày tải lên: 16/03/2014, 06:20

... refinement at the CNS refinement stage. A random sample of 10% of the X-ray data was excluded from the refinement and was taken as the test data set, and the agreement between the calculated and observed structure ... protein atoms. The former propionate displays the conserved conformation in the structures of the Phe35fiTyr mutant and of the wild-type prot...

Ngày tải lên: 08/03/2014, 10:20

... Biotech, Auburn, CA, USA). All research mice at CINVESTAV and Trudeau Institute were eutha- nized by CO 2 narcosis in accordance with the recommenda- tions of the Panel on Euthanasia of the AVMA and ... dependent on the presence of stable homodimers As the mutations in the cytoplasmic region and the a1 -helix of CD38 affected dimer stability upon solubiliz...

Ngày tải lên: 23/03/2014, 12:20

... (5¢-GCCTCCTGCACCACCAACTG-3¢) and GAPDH-R (5¢-CCAGTAGAGGCAGGGATGATGT-3¢). The real-time PCR program was: pre-incubation at 50 °C for 2 min; initial denaturation at 95 °C for 7 min; and 45 cycles at 95 °C for ... used for statistical analysis of the luciferase assay data; values of P < 0.05 were considered significant. Preparation of nuclear extracts and total protein...

Ngày tải lên: 29/03/2014, 00:20

... Bio-Rad) and analyzed with Multianalyst 1.0 software (Bio-Rad). Alternatively, the FLA5000 imaging system (FUJI) and the L Process and Image Gauge software packages were used. The relat- ive magnitude ... Y, Miyazaki M, Namba M, Yamada H & Tanaka T (2001) Loss of nuclear loca- lization of the S100C protein in immortalized human fibroblasts. Radiat Res 155, 20 8–2 14....

Ngày tải lên: 19/02/2014, 18:20

... regulation of alternative splicing, and also controls their subnuclear localization and nucleocytoplasmic transport [ 1–6 ]. Moreover, environmental signals or viral infection can control the phosphorylation ... viral DNA replication [16]. The E2 protein consists of the N-terminal transacti- vation domain and the C-terminal DNA-binding domain. These two functional domains...

Ngày tải lên: 23/03/2014, 06:20

... and presented contrasting and dis- tinct features of myopathic damage. Although the overall condition of the patients deteriorated with time, in both cases an apparent negative selection of abnormal ... Chiara Rampazzo 1 , Elisa Franzolin 1 , Mari-Carmen Lara 2 , Maya R. Vila ` 2 , Ramon Martı ´ 2 and Vera Bianchi 1 1 Department of Biology, University of Padova, Italy 2 In...

Ngày tải lên: 30/03/2014, 02:20

... (forward, 5¢-TCAGCATTGC AAATAACAATCTCA-3¢; reverse, 5¢-CAGTCTGCTCT TTGTTGCACATATAA-3¢), CTGF (forward, 5¢-CA AGCTGCCCGGGAAAT-3¢; reverse, 5¢-GGACCAGGCA GTTGGCTCTA-3¢), JUN (forward, 5¢-GGATCAAGGC GGAGAGGAA-3¢;reverse,5¢-TCCAGCCGGGCGATT-3¢), PLAU ... ⁄ siRNA2, LAMP2 ⁄ siRNA1 and LAMP2 ⁄ siRNA2 are 5¢- UGACUUCCCUGGCCUAUUUUU-3¢,5¢-ACAUUGAGC UCCUCUCUUGUU-3¢,5¢-GAUAAGGUUGCUUCAGU UAUU-3¢ and 5¢...

Ngày tải lên: 07/03/2014, 05:20

... production and thermal stability The VsEndA variants Q69N and K71N and the VcEndA variants N69Q and N71K were expressed in a soluble form at levels comparable with the native endonucleases. The mutations ... Tokunaga H, Arakawa T, Fukada H & Tokunaga M (2006) Opposing effects of NaCl on reversibility and thermal stability of halophilic beta-lactamase from...

Ngày tải lên: 16/03/2014, 06:20

... pastoris,to investigate the effects o f cardiomyopathic-related m utations on the human skeletal Tm. Our data indicate that the main effects of mutations A6 3V, K70T and E180G are mainly related to the overall stability ... (5¢-GAC AAATACTCTGA AGTACTCAAAGATGCCCAG-3¢), SK TM-1R (5¢-CTGGGCATCTTTGAGTAC TTCAGAGTA TTGTC-3¢), SKTM-K70T (5¢-AAAGATGC ACAGGAG ACGCTGGAGCTGGCAGA...

Ngày tải lên: 16/03/2014, 18:20